Team:Lethbridge Canada/notebook march
From 2013hs.igem.org
Name: Marie C, Fiona S, Chris I, Katie T, Wesley M
Date: March 12, 2013
Species: E. coli
Strain: DH5α
Protocol: Ute Kothe
Antibiotic: Kan and Amp
Part Number and Plasmid: R0010 PSB1A2, B0032 PSB1A2, J04650 PSB1AK3, B0031 PSB1A2, B0030 PSB1A2, J04500 PSB1AK3
Results: TBA
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Names: Thomas K, Carissa K, Dylan S, Mark H, Brianna C, Wesley M, Austin K, Kieran Mc, Brooke H
Date: March 13, 2013
Species: E. coli
Strain: DH5α
Protocol: Ute Kothe
Antibiotic: Amp
Part Number and Plasmid: R0010 PSB1A2, B0032 PSB1A2, J04650 PSB1AK3, B0031 PSB1A2, B0030 PSB1A2, J04500 PSB1AK3
Results: TBA
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Names: Mark H, Chris I, Elaine B, Dax L
Date: March 13, 2013
Species: E. coli
Strain: DH5α
Protocol: Ute Kothe
Mini prepped: 3-B0030, 3-B0031, 3-B0032,
2-J04500 and 3-R0010
Glycerol Stocked B0030 in G1, B0031 in G2, B0032 in G3, J04500 in G4 and R0010 in G5
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Names: Patrick O, Steven T, Amrinder G, Yoyo Y, Wesley M, Orion S
Date: March 15, 2013
Species: E. coli
Strain: DH5α
Protocol: Ute Kothe use buffer 4 instead of buffer 10
Restricted: 6-B0030, 6-B0031, 6-B0032,
4-J04500 and 6-R0010
Incubate 1 hour at 37˚C
Stored freezer overnight
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Name: Patrick O
Date: March 16, 2013
Species: E. coli
Strain: DH5α
Protocol: Ute Kothe
Ran 15µL each of 3-B0030, 3-B0031, 3-B0032,
2-J04500 and 3-R0010 through 1% agarose gel
Quantities: 12.5µL DNA and 2.5µL of 6x loading dye (15µL total), 12.5µL 1kb DNA ladder and 2.5µL 6x loading dye (15µL total)
note: ladder difficult to see on gel picture, next time less DNA ladder
Lane #
Contents
Volume(µL)
1
Empty
n/a
2
1kb DNA Ladder
15
3
BBa_B0030
15
4
BBa_B0030
15
5
BBa_B0030
15
6
BBa_B0031
15
7
BBa_B0031
15
8
BBa_B0031
15
9
BBa_B0032
15
10
BBa_B0032
15
11
BBa_B0032
15
12
BBa_R0010
15
13
BBa_R0010
15
14
BBa_R0010
15
15
BBa_J04500
15
16
BBa_J04500
15
17+
empty
n/a
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Names: Fiona S, Katie T, Chris I, Elaine B.
Date: March 18, 2013
Species: E. coli
Strain: DH5α
Protocol: Ute Kothe
Ran 15µL each of 3-B0030, 3-B0031, 3-B0032,
2-J04500 and 3-R0010 through 1% agarose gel
Quantities: 12.5µL DNA and 2.5µL of 6x loading dye (15µL total), 12.5µL 1kb DNA ladder and 2.5µL 6x loading dye (15µL total)
Lane #
Contents
Volume(µL)
1
Empty
n/a
2
1 kb DNA Ladder
6
2
1 kb DNA Ladder
6
3
BBa_B0030
15
4
BBa_B0030
15
5
BBa_B0030
15
6
BBa_B0031
15
7
BBa_B0031
15
8
BBa_B0031
15
9
BBa_B0032
15
10
BBa_B0032
15
11
BBa_B0032
15
12
BBa_R0010
15
13
BBa_R0010
15
14
BBa_R0010
15
15
BBa_J04500
15
16
BBa_J04500
15
17+
Empty
n/a
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Names: Fiona S, Chris I.
Date: March 19, 2013
Species: E. coli
Strain: DH5α
Protocol: Ute Kothe
Transformed 2µL of J04650 pSB1AK3 from Spring 2012 Kit Plate 4 C12 into competent cells.
Quantities: 2µL J04650 pSB1AK3, 200µL LB media
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Names: Yoyo Y, Wesley M
Date: March 20, 2013
Species: E. coli
Strain: DH5α
Protocol: Ute Kothe
Picked cells of J04650 from previous day’s transformation
Colonies didn't form properly within either plate, cells were picked with a dab (single colonies)
Quantities: 3x5ml of media
Antibiotic: Ampicillin
Glycerol Stock: R0010 pSB1A2
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Name: Mark H
Date: March 21, 2013
Species: E. coli
Strain: DH5α
Protocol: Ute Kothe
Miniprepped 1-R0010 used 50uL of Elution Buffer
Glycerol Stocked 1-R0010 in G6
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Name: Yoyo Y
Date: March 27, 2013
Species: E. coli
Strain: DH5α
Protocol: Ute Kothe
Transformed 2µL of J04650, and B0015 into 25µL of DH5α cell
Quantities: 2µL of J04650 and 2µL of B0015 added into 25µL of DH5α cells
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Names: Marie C, Fiona S, Chris I
Date: March 28, 2013
Species: E. coli
Strain: DH5α
Protocol: Ute Kothe
Transformed 2.5µL of J06702
Plasmid: pSB1A2
Quantities: 2.5µL of DNA into 100µL of pSB1A2
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Names: Chris I, Fiona S, Marie C
Date: March 28, 2013
Species: E. coli
Strain: DH5α
Protocol: Ute Kothe
Picked 3 colonies from March 19, J04650
Picked 1 colony from March 26, J04650
Picked 1 colony from March 27, B0015
5mL cultures with 5µL ampicillin.
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Names: Chris I, Fiona S, Marie C
Date: March 29, 2013
Species: E. coli
Strain: DH5α
Protocol: Ute Kothe
Picked 2 colonies from March 28, J06702
5mL cultures with 5µL ampicillin
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Names: Chris I, Fiona S, Marie C
Date: March 29, 2013
Species: E. coli
Strain: DH5A
Protocol: Ute Kothe
Picked, J61002
Picked, J23113
Picked, J23108
Picked, J23114
Picked, J23100
5mL cultures with 5µL ampicillin
Shaken overnight at 37°C
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