Team:Lambert GA

From 2013hs.igem.org

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<li class="first"><a href="https://2013hs.igem.org/Team:Lambert_GA" accesskey="1" title="">Home</a></li>
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                <li><a href="https://2013hs.igem.org/Team:Lambert_GA/team" accesskey="3" title="">Team</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lambert_GA/project" accesskey="2" title="">Project</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lambert_GA/labnotebook" accesskey="4" title="">Lab Notebook</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lambert_GA/resultsconclusions" accesskey="5" title="">Conclusions</a></li>
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                <li><a href="https://2013hs.igem.org/Team:Lambert_GA/futureaspirations" accesskey="8" title="">Future</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lambert_GA/safety" accesskey="5" title="">Safety</a></li>
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                <li><a href="https://2013hs.igem.org/Team:Lambert_GA/attributions" accesskey="6" title="">Attributions</a></li>
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                <li><a href="https://2013hs.igem.org/Team:Lambert_GA/humanpractices" accesskey="7" title="">Human Practices</a></li>           
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                <li><a href="https://2013hs.igem.org/Team:Lambert_GA/activities" accesskey="6" title="">Activities</a></li>             
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<font size=4><center><b><u><h1>Home</h1></u></b></center></font>
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<center><img src="http://www.forsyth.k12.ga.us/cms/lib3/GA01000373/Centricity/Domain/2329/Lambert%20Crest.jpg" width="30%" ></center>
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<font size=4><br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; We are from Lambert High School in Georgia. We are Georgia's first high school iGEM team and we are very excited to be part of this group. Our instructor is Mrs. Janet Standeven, who teaches biology and environmental science at Lambert. We are sponsored by Dr. Mark Styczynski from Georgia Tech's Chemical Engineering department. The members of our iGEM team are very enthusiastic about biotechnology and molecular engineering. We are grateful for opportunity to gain experience in synthetic biology and to have been able to work in research labs at the Georgia Institute of Technology.</font>
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|We are the Lambert High School iGEM team from Forsyth County, Georgia. We are honored to be the first high school iGEM team from Georgia, and this is our first year in the competition.
 
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[[File:iGEM Group Picture.JPG]]
 
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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|align="center"|[[Team:Lambert_GA | Team Lambert_GA]]
 
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{| style="color:#1b2c8a;background-color:#000000;" cellpadding="3" cellspacing="1" border="1" bordercolor="#FFFFFF" width="62%" align="center"
 
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!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=Lambert_GA Official Team Profile]
 
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===Team===
 
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|[[Image:Lambert_GA_logo.png|200px|right|frame]]
 
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We are Georgia's first high school iGEM team. We are from Lamber High School in Forsyth County, Georgia, just outside Atlanta. This is the first year that our school has had an iGEM program, and we are very excited to be part of this group.
 
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Our instructor is Mrs. Janet Standeven, who teaches biology and environmental science at Lambert. We are also being sponsored by Dr. Mark Styczynski from Georgia Tech's Chemical Engineering department.
 
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The members of our iGEM team are very enthusiastic about biotechnology and molecular engineering and are glad to be presented with the opportunity to gain valuable experience in this ever-expanding bio technical field and to have been able to work in doctorate labs at the prestigious Georgia Institute of Technology being just high school students.
 
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In 2010, Georgia Institute of Technology’s iGem team submitted the part K410000, a periplasmic heat generator made with HybB and OmpA and AOX.  The following year several teams used HybB in their projects with mixed results.  Our project is the continuation these projects and the characterization of HybB.  To do this we will put RFP under HybB promotion.  In addition to we will put OmpA AOX under three constituitive promoters. 
 
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The purpose is to determine whether the strength of constituitive promoters will change the amount of heat released by generator OmpA AOX.  With HybB we are hoping to get reliable results from cold shock treatment.  Future applications of this project would allow us to control cell protein expression through temperature conditions.
 
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===Future Aspirations===
 
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By engineering a reliable coldshock promoter we can provide a switch to turn on gene expression using temperature.
 
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===Notebook===
 
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Show us how you spent your days.
 
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      <TH>Date</TH>
 
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      <TH>Accomplishments</TH>
 
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      <TD>January 1-15</TD>
 
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      <TD>We met for the first time during class time as an Instructional Focus. We discussed project ideas and iGEM in general, and gave out protocols to study.</TD>
 
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      <TD>January 16-31</TD>
 
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      <TD> We practiced transformations with p-GLO from Biorad. We learned about Bacterial Antibiotic resistance using Biorad Antibiotic Resistance Lab.</TD>
 
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      <TD>February 1-14</TD>
 
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      <TD>Discussed project ideas.  Planned for visit to Georgia Institute of Technology, Styczynski Lab.  Sold Pretzels for fund raiser.</TD>
 
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      <TD>February 15-28</TD>
 
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      <TD>Studied and took test over iGEM protocols and 3A Assembly.  Finalized list of Biobrick parts of interest.  Outlined initial ideas.  Use K410000 to characterize cold shock heat generator.</TD>
 
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      <TD>March 1-14</TD>
 
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      <TD>March 9 Field trip to GATech. 
 
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Rehydrated J23119, J13002, J04450, K410000
 
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Miniprepped parts
 
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Ran diagnostic gels
 
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Sent parts for sequencing
 
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Calculated concentration of DNA
 
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Met with team members from GATech iGEM.  Discussed the K410000 project. 
 
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New idea for project; isolate HybB and make it a Biobrick.  GATech team will send their frozen stocks.</TD>
 
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      <TD>March 1-14</TD>
 
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===Results/Conclusions===
 
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What did you achieve over the course of your semester?
 
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===Safety===
 
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What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
 
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Whenever the team was performing procedures, everybody wore safety goggles and gloves. We followed procedures very carefully, and we were circumspect with all procedures and cleanup.
 
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We took a safety quiz at the beginning of the competition and we safely worked in a Georgia Tech laboratory.
 
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We were supervised by Mrs. Standeven, Dr. Styczynski, and Mrs. Cochran the entire time we were in the Georgia Tech lab.
 
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===Attributions===
 
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Who worked on what?
 
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Everyone in the Lambert iGem team is motivated and dedicated to enhance the team and our projects. Ms.Standeven, has dedicated many hours of her day to help prepare for upcoming labs.
 
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===Human Practices===
 
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What impact does/will your project have on the public? 
 
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===Fun!===
 
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What was your favorite team snack?? Have a picture of your team mascot?
 
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Our favorite team snack is Pretzels.
 
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Latest revision as of 22:00, 21 June 2013