Team:NC School of Sci Math

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{{NCSSM_iGEM_13|
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__NOTOC__
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===North Carolina School of Science and Mathematics===
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The North Carolina School of Science and Mathematics is a public, residential, coeducational high school for juniors and seniors with high intellectual ability and determination to prepare for professional careers and commitment to scholarship. It was established by the North Carolina General Assembly in 1978 to provide challenging educational opportunities for students with special interests and potential in the sciences and mathematics. NCSSM places great emphasis on opportunities for student research, working with local universities and fostering an environment that encourages students to ask questions about the world around them.
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{|
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===Executive Summary===
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<html><img style="float:left; width:240px; margin-right:10px;" src="http://www.med.unc.edu/cfpulmcenter/images/ribeiro-lab/JAllen.jpg"></html>Biosensors provide a wide variety of applications, particularly analyte detection in the environment. Positive results in biosensors lead to the output of a reporter, commonly in the form of fluorescent proteins. Here we construct a biosensor that is capable of indicating the presence of various pollutants in water through expression of several different fluorescent proteins. We selected lead, copper, phosphate, and nitrate/nitrite promoters and paired them with specific reporter coding sequences. Presence of these ions drives the transcription of specific fluorescent proteins. Detection of these proteins is enabled through a light detection apparatus and the information can be sent to mobile devices in a user-friendly interface via a modified Google ADK. This novel multibiosensor can be implemented to detect pollutants in sewer systems, septic tanks, and other sources of water, and provide and early-detection warning system, preventing the pollutants from causing serious harm to equipment, animals, or people.
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<font face="verdana" style="color:#00008B"><center>NCSSM iGEM 2013
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<br><center>[[Team:Caltech| <font face="verdana" style="color:#00008B"> '''Home''' </font>]] <br><br>
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[[Team:Caltech/Members | <font face="verdana" style="color:#00008B"> '''People''' </font>]] <br><br>
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[[Team:Caltech/Project | <font face="verdana" style="color:#00008B"> '''Project Details''' </font>]] <br><br>
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[[Team:Caltech/Protocols | <font face="verdana" style="color:#00008B"> '''Protocols''' </font>]] <br><br>
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[[Team:Caltech/Parts | <font face="verdana" style="color:#00008B"> '''Completed Systems''' </font>]] <br><br>
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[[Team:Caltech/Biosafety | <font face="verdana" style="color:#00008B"> '''Biosafety''' </font>]] <br><br>
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[[Team:Caltech/Acknowledgments | <font face="verdana" style="color:#00008B"> '''Support''' </font>]] <br><br>
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|align="left"|[[Team:NC_School_of_Sci_Math | Team NC_School_of_Sci_Math]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=NC_School_of_Sci_Math Official Team Profile]
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===Project===
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Biosensors provide a wide variety of applications, particularly analyte detection in the environment. Positive results in biosensors lead to the output of a reporter, commonly in the form of fluorescent proteins. Here we construct a biosensor that is capable of indicating the presence of various pollutants in water through expression of several different fluorescent proteins. We selected lead, copper, phosphate, and nitrate/nitrite promoters and paired them with specific reporter coding sequences. Presence of these ions drives the transcription of specific fluorescent proteins. Detection of these proteins is enabled through a light detection apparatus and the information can be sent to mobile devices in a user-friendly interface via a modified Google ADK. This novel multibiosensor can be implemented to detect pollutants in sewer systems, septic tanks, and other sources of water, and provide and early-detection warning system, preventing the pollutants from causing serious harm to equipment, animals, or people.
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<div style="float: right; clear: right; margin-top:-5px;">[[Image:unicorn.jpg]]</div>
The goal of this project, therefore, was to develop a multi-input logic gate in Escherichia Coli, which can detect the presence of a number of environmentally degrading compounds, and for each, produce a unique colorimetric output. We applied some principles of electrical engineering by using a Google ADK to sense this colorimetric output and send an alert. We envision that for an end-user, our elegant synthetic biology solution will allow a homeowner to easily and effectively be notified of the need for inspection of their septic system. The product has applications in water quality tests, sewage treatments plants, and in industry where the presence of pollutants could be harmful to equipment, workers, or consumers.
The goal of this project, therefore, was to develop a multi-input logic gate in Escherichia Coli, which can detect the presence of a number of environmentally degrading compounds, and for each, produce a unique colorimetric output. We applied some principles of electrical engineering by using a Google ADK to sense this colorimetric output and send an alert. We envision that for an end-user, our elegant synthetic biology solution will allow a homeowner to easily and effectively be notified of the need for inspection of their septic system. The product has applications in water quality tests, sewage treatments plants, and in industry where the presence of pollutants could be harmful to equipment, workers, or consumers.
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{{clear}}
 
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===Notebook===
 
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To begin our experiment, we needed to create a device that could detect color. We chose to use the Google ADK, an open ended platform that allows users to take control of many sensors that come attached to an Arduino board, including a colorimeter. However, a standard ADK is set up to match any color presented to it, using LED lights. In some of our preliminary tests, the reflection of the light from these LEDs skewed data. Therefore, we modified the code of the ADK, using Google's software developer package, so that it does not turn on its LED lights but instead outputs colorimeter data to a computer. An example of this output is shown below.
 
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As a foray into our project, we first took some preliminary data using our modified Google ADK and pGLO E. coli expressing Green Fluorescent Protein. We found that we were able to detect a distinct change in color. Some baseline color was present from the UV Light source used for the experiment. The graphs below clearly show that our system is able to detect E. coli expressing GFP.
 
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[[File:pGLOGraphs.png|960px]]
 
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Small LED lights were used to test the ADK's ability to detect red, blue, and yellow lights. The results showed the same trends as the GFP test, indicating that we would be able to distinguish between several different colors expressed. In general, red, green, and blue lights show dramatic increases in the intensity of that color, while the other two colors remain about the same. Yellow light however, increase both red and green light, while blue remains at the same level. From these observations, we are able to identify which type of light is shown based only on the data collected.
 
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===Results/Conclusions===
 
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What did you achieve over the course of your semester?
 
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===Safety===
 
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What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
 
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===Attributions===
 
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Who worked on what?
 
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===Human Practices===
 
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What impact does/will your project have on the public? 
 
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===Fun!===
 
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What was your favorite team snack?? Have a picture of your team mascot?
 
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{{clear}}
 
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For more details, please see our [[Team:Caltech/Project|<font style="color:#BB4400">project</font>]] page.
 
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===References===
 
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# Hooper LV, Midtvedt T, and Gordon JI. '''How host-microbial interactions shape the nutrient environment of the mammalian intestine'''. ''Annu Rev Nutr'' 2002; 22 283-307.
 
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#  Mazmanian SK, Liu CH, Tzianabos AO, and Kasper DL. '''An immunomodulatory molecule of symbiotic bacteria directs maturation of the host immune system'''. ''Cell'' 2005 Jul 15; 122(1) 107-18.
 
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# Donskey CJ. '''The role of the intestinal tract as a reservoir and source for transmission of nosocomial pathogens'''. ''Clin Infect Dis'' 2004 Jul 15; 39(2) 219-26.
 
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# Suttie JW. '''The importance of menaquinones in human nutrition'''. ''Annu Rev Nutr'' 1995; 15 399-417.
 
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# Krammer HJ, Kamper H, von Bunau R, Zieseniss E, Stange C, Schlieger F, Clever I, and Schulze J. '''Probiotic drug therapy with E. coli strain Nissle 1917 (EcN): results of a prospective study of the records of 3,807 patients'''. ''Z Gastroenterol'' 2006 Aug; 44(8) 651-6.
 
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# Westendorf AM, Gunzer F, Deppenmeier S, Tapadar D, Hunger JK, Schmidt MA, Buer J, and Bruder D. '''Intestinal immunity of Escherichia coli NISSLE 1917: a safe carrier for therapeutic molecules'''. ''FEMS Immunol Med Microbiol'' 2005 Mar 1; 43(3) 373-84.
 
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# Rao S, Hu S, McHugh L, Lueders K, Henry K, Zhao Q, Fekete RA, Kar S, Adhya S, and Hamer DH. '''Toward a live microbial microbicide for HIV: commensal bacteria secreting an HIV fusion inhibitor peptide'''. ''Proc Natl Acad Sci U S A'' 2005 Aug 23; 102(34) 11993-8.
 
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# Duan F and March JC. '''Interrupting Vibrio cholerae infection of human epithelial cells with engineered commensal bacterial signaling'''. ''Biotechnol Bioeng'' 2008 Sep 1; 101(1) 128-34.
 
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Latest revision as of 03:43, 22 June 2013

Name.png
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2013

North Carolina School of Science and Mathematics

The North Carolina School of Science and Mathematics is a public, residential, coeducational high school for juniors and seniors with high intellectual ability and determination to prepare for professional careers and commitment to scholarship. It was established by the North Carolina General Assembly in 1978 to provide challenging educational opportunities for students with special interests and potential in the sciences and mathematics. NCSSM places great emphasis on opportunities for student research, working with local universities and fostering an environment that encourages students to ask questions about the world around them.

Executive Summary

Biosensors provide a wide variety of applications, particularly analyte detection in the environment. Positive results in biosensors lead to the output of a reporter, commonly in the form of fluorescent proteins. Here we construct a biosensor that is capable of indicating the presence of various pollutants in water through expression of several different fluorescent proteins. We selected lead, copper, phosphate, and nitrate/nitrite promoters and paired them with specific reporter coding sequences. Presence of these ions drives the transcription of specific fluorescent proteins. Detection of these proteins is enabled through a light detection apparatus and the information can be sent to mobile devices in a user-friendly interface via a modified Google ADK. This novel multibiosensor can be implemented to detect pollutants in sewer systems, septic tanks, and other sources of water, and provide and early-detection warning system, preventing the pollutants from causing serious harm to equipment, animals, or people.

Unicorn.jpg

The goal of this project, therefore, was to develop a multi-input logic gate in Escherichia Coli, which can detect the presence of a number of environmentally degrading compounds, and for each, produce a unique colorimetric output. We applied some principles of electrical engineering by using a Google ADK to sense this colorimetric output and send an alert. We envision that for an end-user, our elegant synthetic biology solution will allow a homeowner to easily and effectively be notified of the need for inspection of their septic system. The product has applications in water quality tests, sewage treatments plants, and in industry where the presence of pollutants could be harmful to equipment, workers, or consumers.

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