Team:St Pauls London/Abstract

From 2013hs.igem.org

(Difference between revisions)
 
(7 intermediate revisions not shown)
Line 38: Line 38:
         #catlinks {
         #catlinks {
                 display: none;
                 display: none;
-
         }
+
         }
/*----- HEADER -----*/
/*----- HEADER -----*/
Line 45: Line 45:
height: 150px;
height: 150px;
}
}
-
 
+
-
     
+
#headerTitle {
#headerTitle {
text-align: right;
text-align: right;
Line 99: Line 98:
color: #F93;
color: #F93;
text-decoration: underline;
text-decoration: underline;
-
}
+
}    
-
 
+
-
         a.no-decoration {
+
         a.mobile-link, a.mobile-link:hover, a.mobile-link:active {
-
                 text-decoration: none;
+
                 text-decoration:none;
-
                color: inherit;
+
         }
         }
Line 126: Line 124:
         }
         }
-
         ul.drop-down li.top-of-list {
+
         div.drop-down-top {
-
                display: block;
+
                 background-color: transparent;
                 background-color: transparent;
-
                 color: black;
+
                 margin-bottom: -7px;
-
                 text-decoration: none;
+
        }
 +
 
 +
        ul.drop-down:hover div.drop-down-top {
 +
                 background-color: #EAEAEA;
 +
        }
 +
 
 +
        ul.drop-down li.top-of-list {  /*----- makes sure top item is always shown -----*/
 +
                display: block;
         }
         }
                
                
Line 139: Line 143:
         ul.drop-down:hover li.top-of-list {
         ul.drop-down:hover li.top-of-list {
                 color: #F93;
                 color: #F93;
-
text-decoration: underline;
+
text-decoration: underline;            
         }
         }
Line 145: Line 149:
/*----- MAIN CONTENT -----*/
/*----- MAIN CONTENT -----*/
-
 
-
        .indent-content {
 
-
                margin-left: 25px;
 
-
        }
 
-
 
-
        .pdf {
 
-
                text-align: center;
 
-
        }
 
.title-link, .title-link:active, .title-link:visited {
.title-link, .title-link:active, .title-link:visited {
Line 204: Line 200:
<html>
<html>
   <ul class="drop-down">
   <ul class="drop-down">
 +
  <div class="drop-down-top">
   <li class="top-of-list">
   <li class="top-of-list">
     <a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London">
     <a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London">
Line 211: Line 208:
HOME
HOME
<html>
<html>
-
   </p></div></a></li></ul>
+
   </p></div></a></li></div></ul>
</html>
</html>
|
|
<html>
<html>
   <ul class="drop-down">
   <ul class="drop-down">
-
   <li class="top-of-list">
+
  <div class="drop-down-top">
 +
   <li class="top-of-list"> 
 +
    <a class="mobile-link" href="">
     <div class="nav_button">
     <div class="nav_button">
     <p class="nav_buttonText">
     <p class="nav_buttonText">
Line 222: Line 221:
PROJECT
PROJECT
<html>
<html>
-
   </p></li></div>
+
   </p></div></a></li></div>
</html>
</html>
<html><div class="drop-down"><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Abstract"></html>
<html><div class="drop-down"><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Abstract"></html>
 +
----
ABSTRACT
ABSTRACT
----
----
Line 244: Line 244:
<html>
<html>
   <ul class="drop-down">
   <ul class="drop-down">
 +
  <div class="drop-down-top">
   <li class="top-of-list">
   <li class="top-of-list">
 +
    <a class="mobile-link" href="">
     <div class="nav_button">
     <div class="nav_button">
     <p class="nav_buttonText">
     <p class="nav_buttonText">
Line 250: Line 252:
THE TEAM
THE TEAM
<html>
<html>
-
   </li></p></div>
+
   </p></div></a></li></div>
</html>
</html>
<html><div class="drop-down"><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Participants"></html>
<html><div class="drop-down"><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Participants"></html>
 +
----
PARTICIPANTS
PARTICIPANTS
----
----
Line 264: Line 267:
<html>
<html>
   <ul class="drop-down">
   <ul class="drop-down">
 +
  <div class="drop-down-top">
   <li class="top-of-list">
   <li class="top-of-list">
 +
    <a style="text-decoration: none" href="">
     <div class="nav_button">
     <div class="nav_button">
     <p class="nav_buttonText">
     <p class="nav_buttonText">
Line 270: Line 275:
OTHER
OTHER
<html>
<html>
-
   </li></p></div>
+
   </p></div></a></li></div>
</html>
</html>
<html><div class="drop-down"><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Notebook"></html>
<html><div class="drop-down"><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Notebook"></html>
 +
----
NOTEBOOK
NOTEBOOK
----
----
Line 282: Line 288:
<html><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Safety"></html>
<html><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Safety"></html>
SAFETY
SAFETY
 +
----
 +
<html></a></li></html>
 +
<html><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Attributions"></html>
 +
ATTRIBUTIONS
----
----
<html></a></li></html>
<html></a></li></html>
Line 296: Line 306:
<html><div style="text-align:justify; margin-right: 50px;"></html>
<html><div style="text-align:justify; margin-right: 50px;"></html>
-
:This year, our aim is to mutate E.coli to create a semi-quantitative lactose detector; we intend to create three mutant operons on a single plasmid, with each operon responding to a different concentration of lactose in the environment the E.coli finds itself in.
+
:This year, our aim is to mutate E.coli to create a semi-quantitative lactose biosensor; we intend to create three mutant operons on a single plasmid, with each operon responding to a different concentration of lactose in the environment the E.coli finds itself in. We plan to use red, yellow and green fluorescent proteins in a “traffic light” system, whereby the colour of the protein translated corresponds to the concentration of lactose, with red fluorescent protein denoting a high concentration of lactose, yellow fluorescent protein representing a low concentration of lactose and green fluorescent protein showing that there is no lactose present. It is our hope that this could potentially be used in allergy testing and in the analysis of food samples, and by a spectrum individuals who are lactose intolerant.
-
 
+
-
 
+
-
:We plan to use red, yellow and green fluorescent proteins in a “traffic light” system, whereby the colour of the protein translated corresponds to the concentration of lactose, with red fluorescent protein denoting a high concentration of lactose, yellow fluorescent protein representing a low concentration of lactose and green fluorescent protein showing that there is no lactose present in the immediate environment the E.coli is exposed to. It is our hope that this could potentially be used in allergy testing and in the analysis of food samples, and by individuals who are lactose intolerant; because we hope to design a semi-quantitative detector it will show a spectrum of lactose concentrations so mild intolerants and severe intolerants will be able to use the sensor in different ways.
+
-
 
+
-
 
+
-
:So far we have been designing our project and selecting appropriate biobricks for use in each operon. We have also been using the 3A assembly kit to practice assembling biobricks on a plasmid backbone. During the past year, the team has also been involved in a plasmid mutagenesis project to modify T4 bacteriophage lysozyme, and in so doing we have practiced many of the lab techniques needed for biobrick assembly.
+
<html></div></html>
<html></div></html>

Latest revision as of 11:44, 7 June 2013

Abstract

This year, our aim is to mutate E.coli to create a semi-quantitative lactose biosensor; we intend to create three mutant operons on a single plasmid, with each operon responding to a different concentration of lactose in the environment the E.coli finds itself in. We plan to use red, yellow and green fluorescent proteins in a “traffic light” system, whereby the colour of the protein translated corresponds to the concentration of lactose, with red fluorescent protein denoting a high concentration of lactose, yellow fluorescent protein representing a low concentration of lactose and green fluorescent protein showing that there is no lactose present. It is our hope that this could potentially be used in allergy testing and in the analysis of food samples, and by a spectrum individuals who are lactose intolerant.

Retrieved from "http://2013hs.igem.org/Team:St_Pauls_London/Abstract"