Team:St Pauls London/Parts

From 2013hs.igem.org

(Difference between revisions)
(Created page with "temporary")
(→Operon 3)
 
(47 intermediate revisions not shown)
Line 1: Line 1:
-
temporary
+
<html> <!-- STYLES -->
 +
  <head>
 +
 
 +
    <link href='http://fonts.googleapis.com/css?family=Open+Sans:300' rel='stylesheet' type='text/css'>
 +
   
 +
    <style>
 +
        body {
 +
                font-family: 'Open Sans';
 +
                font-weight: 300;
 +
                letter-spacing: 0.5px;
 +
        }
 +
 
 +
        #p-logo {
 +
                display: none;
 +
        }
 +
 
 +
        form#searchform {
 +
                display: none;
 +
        }
 +
 
 +
        h1.firstHeading {
 +
                display: none;
 +
        }
 +
 
 +
        #top-of-page {
 +
                position: absolute;
 +
                margin-top: -125px;
 +
                margin-left: -5px;
 +
                height: 120px;
 +
                width: 975px;
 +
                background-image: url('https://static.igem.org/mediawiki/2013hs/b/b5/Banner.jpg');
 +
        }
 +
 
 +
        #toc {
 +
                display: none;
 +
        }
 +
 
 +
        #catlinks {
 +
                display: none;
 +
        }
 +
 
 +
/*----- HEADER -----*/
 +
#header {
 +
                padding-top: 20px;
 +
height: 150px;
 +
}
 +
 +
#headerTitle {
 +
text-align: right;
 +
font-weight: 400;
 +
font-size: 44px;
 +
                color: black;
 +
}
 +
 
 +
#logo {
 +
left: 20%;
 +
align: left;
 +
width: 120px;
 +
height: 120px;
 +
}
 +
 +
#headerSubTitle {
 +
font-size: 14px;
 +
letter-spacing: 2px;
 +
color: #666;
 +
}
 +
 +
a#igem, a#igem:visited {
 +
color: inherit;
 +
text-decoration: none;
 +
}
 +
 +
a#igem:hover, a#igem:active {
 +
color: #F93;
 +
text-decoration: underline;
 +
}
 +
 
 +
/*----- HEADER BUTTONS -----*/
 +
 
 +
.nav_button {
 +
bottom: 20%;
 +
display: table;
 +
}
 +
 +
.nav_buttonText {
 +
color: #222;
 +
text-align: center;
 +
vertical-align: middle;
 +
display: table-cell;
 +
                font-weight: 100;
 +
}
 +
 
 +
        a.nav_buttonLink, a.nav_buttonLink:visited {
 +
text-decoration: none;
 +
                color: black;
 +
}
 +
a.nav_buttonLink:hover, a.nav_buttonLink:active {
 +
color: #F93;
 +
text-decoration: underline;
 +
}   
 +
 +
        a.mobile-link, a.mobile-link:hover, a.mobile-link:active {
 +
                text-decoration:none;
 +
        }
 +
 
 +
/*----- END OF BUTTONS -----*/
 +
 
 +
        ul.drop-down {
 +
                list-style: none;
 +
                margin-top: 0px;
 +
                position: absolute;
 +
                padding: 0;
 +
        }
 +
 
 +
        ul.drop-down li {
 +
                display: none;
 +
                position: relative;
 +
                text-align: left;
 +
                margin: 5px;               
 +
        }
 +
 
 +
        div.drop-down {
 +
                background-color: #EAEAEA;
 +
        }
 +
 
 +
        div.drop-down-top {
 +
                background-color: transparent;
 +
                margin-bottom: -7px;
 +
        }
 +
 
 +
        ul.drop-down:hover div.drop-down-top {
 +
                background-color: #EAEAEA;
 +
        }
 +
 
 +
        ul.drop-down li.top-of-list {  /*----- makes sure top item is always shown -----*/
 +
                display: block;
 +
        }
 +
             
 +
        ul.drop-down:hover li {  /*----- shows drop down -----*/
 +
                display: block;             
 +
        }
 +
 
 +
        ul.drop-down:hover li.top-of-list {
 +
                color: #F93;
 +
text-decoration: underline;             
 +
        }
 +
 
 +
/*----- END OF HEADER -----*/
 +
 
 +
/*----- MAIN CONTENT -----*/
 +
 
 +
.title-link, .title-link:active, .title-link:visited {
 +
text-decoration: none;
 +
color: inherit;
 +
}
 +
 +
.title-link:hover {
 +
                text-decoration: underline;
 +
                color: #F93;
 +
        }
 +
 
 +
</style>
 +
  </head>
 +
</html>
 +
 
 +
<html>
 +
  <div id="top-of-page">
 +
 
 +
  </div>
 +
 
 +
  <div id="header">
 +
  <div id="push-left">
 +
  <!--<img src="assets/logo.png" id="logo">-->
 +
  <div id="headerTitle">
 +
</html>
 +
<center>SPS_London</center>
 +
<html>
 +
  </div>
 +
</html>
 +
 
 +
<html>
 +
<div id="headerSubTitle">
 +
</html>
 +
<center>TMNP Collective</center>
 +
<html>
 +
  </div>
 +
  <div id="headerSubTitle">
 +
</html>
 +
<center>An <html><a id="igem" href="https://2013hs.igem.org/Main_Page"></html>iGEM<html></a></html> Team</center>
 +
 
 +
<html>
 +
  </div>
 +
  </div>
 +
</html>
 +
 
 +
<center>
 +
{| style="text-align: center; width: 500px;"
 +
|
 +
 
 +
<html>
 +
  <ul class="drop-down">
 +
  <div class="drop-down-top">
 +
  <li class="top-of-list">
 +
    <a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London">
 +
    <div class="nav_button">
 +
    <p class="nav_buttonText">
 +
</html>
 +
HOME
 +
<html>
 +
  </p></div></a></li></div></ul>
 +
</html>
 +
|
 +
<html>
 +
  <ul class="drop-down">
 +
  <div class="drop-down-top">
 +
  <li class="top-of-list"> 
 +
    <a class="mobile-link" href="">
 +
    <div class="nav_button">
 +
    <p class="nav_buttonText">
 +
</html>
 +
PROJECT
 +
<html>
 +
  </p></div></a></li></div>
 +
</html>
 +
<html><div class="drop-down"><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Abstract"></html>
 +
----
 +
ABSTRACT
 +
----
 +
<html></a></li></html>
 +
<html><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Parts"></html>
 +
PARTS
 +
----
 +
<html></a></li></html>
 +
<html><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Procedures"></html>
 +
PROCEDURES
 +
----
 +
<html></a></li></html>
 +
<html><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Protocols"></html>
 +
PROTOCOLS
 +
----
 +
<html></a></li></html>
 +
<html></div></ul></html>
 +
|
 +
<html>
 +
  <ul class="drop-down">
 +
  <div class="drop-down-top">
 +
  <li class="top-of-list">
 +
    <a class="mobile-link" href="">
 +
    <div class="nav_button">
 +
    <p class="nav_buttonText">
 +
</html>
 +
THE TEAM
 +
<html>
 +
  </p></div></a></li></div>
 +
</html>
 +
<html><div class="drop-down"><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Participants"></html>
 +
----
 +
PARTICIPANTS
 +
----
 +
<html></a></li></html>
 +
<html><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Supervisors"></html>
 +
SUPERVISORS
 +
----
 +
<html></a></li></html>
 +
<html></div></html>
 +
|
 +
<html>
 +
  <ul class="drop-down">
 +
  <div class="drop-down-top">
 +
  <li class="top-of-list">
 +
    <a style="text-decoration: none" href="">
 +
    <div class="nav_button">
 +
    <p class="nav_buttonText">
 +
</html>
 +
OTHER
 +
<html>
 +
  </p></div></a></li></div>
 +
</html>
 +
<html><div class="drop-down"><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Notebook"></html>
 +
----
 +
NOTEBOOK
 +
----
 +
<html></a></li></html>
 +
<html><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Human_Practices"></html>
 +
HUMAN PRACTICES
 +
----
 +
<html></a></li></html>
 +
<html><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Safety"></html>
 +
SAFETY
 +
----
 +
<html></a></li></html>
 +
<html><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Attributions"></html>
 +
ATTRIBUTIONS
 +
----
 +
<html></a></li></html>
 +
<html></div></html>
 +
|-
 +
|}
 +
</center>
 +
 
 +
<html>
 +
  </div>
 +
</html>
 +
 
 +
===Parts===
 +
 
 +
<html>
 +
  <div class="indent-content">
 +
</html>
 +
 
 +
====Circuits====
 +
 
 +
======Operon 1======
 +
<center>
 +
<html>
 +
  <img src="https://static.igem.org/mediawiki/2013hs/archive/a/a4/20130506153956!Operon_1.png"/>
 +
</html>
 +
</center>
 +
 
 +
======Operon 2======
 +
<center>
 +
<html>
 +
  <img src="https://static.igem.org/mediawiki/2013hs/9/9c/Operon_2.png"/>
 +
</html>
 +
</center>
 +
 
 +
======Operon 3======
 +
<center>
 +
<html>
 +
  <img src="https://static.igem.org/mediawiki/2013hs/2/2e/Operon_3_new.png"/>
 +
</html>
 +
</center>
 +
 
 +
====Parts====
 +
 
 +
In our notebook and on our procedures sheet, we refer to parts by letter. The main reason for applying arbitrary letter coded to all parts was to facilitate labelling of plates and tubes. We have included a table (below) with the full BioBrick equivalent of each letter-coded part, as well as its function. This may help other teams looking at our circuits and procedures better understand which parts perform which function.
 +
 
 +
<center>
 +
 
 +
{|class="wikitable" style="text-align: center"
 +
!colspan="6" | PARTS
 +
 
 +
|-
 +
|colspan="2" style="font-weight: bold"|Operon
 +
|style="font-weight: bold"|Letter Code
 +
|style="font-weight: bold"|BioBrick Reference
 +
|style="font-weight: bold"|Function
 +
|style="font-weight: bold"|Length (bp)
 +
|-
 +
|colspan="2" rowspan="3"|2
 +
|J
 +
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K091104 BBa_K091104]
 +
|Promoter
 +
|87
 +
|-
 +
|K
 +
|[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]
 +
|RBS
 +
|12
 +
|-
 +
|L
 +
|[http://partsregistry.org/Part:BBa_E0030 BBa_E0030]
 +
|YFP gene
 +
|723
 +
|-
 +
|2
 +
|3
 +
|C
 +
|[http://partsregistry.org/Part:BBa_C0040 BBa_C0040]
 +
|TetR gene
 +
|685
 +
|-
 +
|2
 +
|3
 +
|F
 +
|[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
 +
|Terminator
 +
|129
 +
|-
 +
|colspan="2" rowspan="4"|3
 +
|A
 +
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K418003 BBa_K418003]
 +
|Promoter
 +
|1416
 +
|-
 +
|B
 +
|[http://partsregistry.org/Part:BBa_B0033 BBa_B0033]
 +
|RBS
 +
|11
 +
|-
 +
|D
 +
|[http://partsregistry.org/Part:BBa_C0072 BBa_C0072]
 +
|mnt gene
 +
|313
 +
|-
 +
|E
 +
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_E1010 BBa_E1010]
 +
|RFP gene
 +
|706
 +
|}
 +
</center>
 +
 
 +
====Backbones====
 +
 
 +
<center>
 +
 
 +
{|class="wikitable" style="text-align: center"
 +
 
 +
|-
 +
|style="font-weight: bold"|Backbone
 +
|style="font-weight: bold"|Length (bp)
 +
|-
 +
|Ampicillin-Resistant
 +
|2155
 +
|-
 +
|Kanamycin-Resistant
 +
|2204
 +
|-
 +
|Chloramphenicol-Resistant
 +
|2070
 +
|-
 +
|Tetracycline-Resistant
 +
|2461
 +
|-
 +
|Ampicillin- & Kanamycin-Resistant
 +
|3189
 +
|-
 +
|}
 +
 
 +
</center>
 +
<html></div></html>

Latest revision as of 19:28, 19 June 2013

Contents

Parts

Circuits

Operon 1

Operon 2

Operon 3

Parts

In our notebook and on our procedures sheet, we refer to parts by letter. The main reason for applying arbitrary letter coded to all parts was to facilitate labelling of plates and tubes. We have included a table (below) with the full BioBrick equivalent of each letter-coded part, as well as its function. This may help other teams looking at our circuits and procedures better understand which parts perform which function.

PARTS
Operon Letter Code BioBrick Reference Function Length (bp)
2 J [http://partsregistry.org/wiki/index.php?title=Part:BBa_K091104 BBa_K091104] Promoter 87
K [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] RBS 12
L [http://partsregistry.org/Part:BBa_E0030 BBa_E0030] YFP gene 723
2 3 C [http://partsregistry.org/Part:BBa_C0040 BBa_C0040] TetR gene 685
2 3 F [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] Terminator 129
3 A [http://partsregistry.org/wiki/index.php?title=Part:BBa_K418003 BBa_K418003] Promoter 1416
B [http://partsregistry.org/Part:BBa_B0033 BBa_B0033] RBS 11
D [http://partsregistry.org/Part:BBa_C0072 BBa_C0072] mnt gene 313
E [http://partsregistry.org/wiki/index.php?title=Part:BBa_E1010 BBa_E1010] RFP gene 706

Backbones

Backbone Length (bp)
Ampicillin-Resistant 2155
Kanamycin-Resistant 2204
Chloramphenicol-Resistant 2070
Tetracycline-Resistant 2461
Ampicillin- & Kanamycin-Resistant 3189

Retrieved from "http://2013hs.igem.org/Team:St_Pauls_London/Parts"