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From 2013hs.igem.org

Safety

1. All procedures were carried out in a clean area. This area was set up as detailed below, and ensured that no external microorganisms contaminated plates or tubes.

• Bunsen burners were on at all times, such that a updraft was always present.
• Prior to any procedures taking place, the entire area which was to be used was scrubbed down with antiseptic.

2. All waste was disposed of in separate, marked bins. The contents of these were then incinerated.

3. Antimicrobial hand wash was systematically used upon entering and leaving the lab to ensure no genetically modified microorganisms could be released outside of the one projects labs in which procedures were carried out.

4. Sterile gloves were worn at all times.

5. Safety glasses were worn at all times.

Letter Code for Part	BioBrick Reference	Function	Organism Derived From	Risk Group
A	BBa_K418003	Promoter	E. coli strain NEB 10-beta	1
B	BBa_B0033	RBS	E. coli strain NEB 10-beta	1
C	BBa_C0040	TetR gene	E. coli strain NEB 10-beta	1