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                  <td width="94%"  style="font-size:24px;color:#00904b; ">iGEM Safety Questions</td>
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          <td width="86%" bgcolor="#F8F8F8" class="STYLE1"><h1>&nbsp;</h1>
            <p><strong>1. Would any of your project  ideas raise safety issues in terms of:</strong><br />
                  <strong>RESEARCHER SAFETY</strong><br />
                We have not worked with any materials that  raise risk to researchers' safety or health. We only worked with experiments  based on commonly used E. coli and baking yeast strains. We performed our  experiment mostly in another institute BGI nearby, their lab fulfill the  requirement of Lab Biosafety level 2, which enable us to perform bacterial  (E.coli) experiment safely.<br />
                Sometime our researchers have to face  regular bio-safety challenges in a molecular microbiology lab, such as staining  DNA agarose gel with EB, each member of our team is careful to use gloves,  goggles, and lab coats in order to protect us .There is also a container for us  to pour culture fluid and culture medium full of cells in, and thus it will be  treated properly afterwards.<br />
                Before the real experiment each member of  our team took 2 weeks to receive training. Any researchers conducting  experiment were instructed about the right method both by printed protocols and  in the lab. After the training, we passed an on-line test about the lab safety,  standard experiment procedures and lab waste treatment. During any experiments,  necessary individual protections such as gloves and white robes were strictly  required. Also, the public safety of the lab is guaranteed by the strict  registering protocols on the operation of any apparatus. In order to make sure  all the requirements of safety were carried out well by the researchers and to  get prepared for any emergency, our lab set up 24hr*7 camera system in the  rooms.<br />
                <strong>PUBLIC SAFETY</strong><br />
                As we said, what we used are non-pathogenic  E. coli strains. We will use the experimental plasmid containing antibiotics. So  one of the security risks is that antibiotic resistance gene may be transferred  to other pathogenic strains and cause the public danger.<br />
                To avoid it happening, we gather all the  culture fluid and culture medium with cells for unified treatment and not let  cells get out of our lab through anyway. <br />
                <strong>ENVIRONMENTAL SAFETY</strong><br />
                We take much care of the environmental  safety. We make sure no media containing engineered cells go into the sewer  before sterilization, and all utensil contacted with the engineered bacteria  will be sterilized. Toxic and hazardous chemicals are collected and treated  properly according to the safety rules of our lab.<br />
                <br />
                <strong>2. Do any of the new  BioBrick parts (or devices) that you made this year raise safety issues?</strong><br />
                NO. We don’t think that our new devices  will raise safety issues. Most of our BioBricks have been used by former iGEM  projects and proved to be innocuous too.<br />
                <br />
                <strong>3. Is there a local  biosafety group, committee, or review board at your institution? </strong><br />
                <strong>If yes, what does your local biosafety group think  about your project? </strong><br />
                <strong>If no, which specific biosafety rules or guidelines do  you have to consider in your country? </strong><br />
                <strong>BIOSAFETY PROVISIONS</strong><br />
                Our experiment is designed and carried out  following the standards of national biosafety office, therefore no laws or  regulations regarding the biosafety issue are violated.<br />
                Because our school (Shenzhen Middle School)  has only a brand new laboratory which is established in 2011, so far, we do not  have a biosafety committee in our institute. We performed our experiment mostly  in another institute BGI nearby, their lab fulfill the requirement of Lab  Biosafety level 2, which enable us to perform bacterial (E.coli) experiment  safely. Many teachers and assistants from biology and chemistry department of our  school care our safety and have checked the laboratory we used and the  processes we have done. And they think the condition we were working is safe  and reliable.<br />
              <br />
                <strong>1.Do  you have any other ideas how to deal with safety issues that could be</strong><strong>useful  for future iGEM competitions? How could parts, devices and systems be made even  safer through biosafety engineering? </strong><br />
                <br />
                Yes. We suggest the registry of standard  biological parts should have a dedicated safety page of every part to ensure  that users would not raise any safety issue when using them. And these data  should be presented when we are searching for a certain part and found its  instructions. By this act, we can at least prevent the safety issues lurking in  the shadows before they could appear.<strong> </strong></p></td>
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