Team:CIDEB-UANL Mexico/Project-Circuit

From 2013hs.igem.org

(Difference between revisions)
Line 60: Line 60:
<br>
<br>
<b>In summary:</b> The Vip3Ca3 production is regulated by temperature: it is produced below the 32ºC and at higher temperatures it is turned off.<br>
<b>In summary:</b> The Vip3Ca3 production is regulated by temperature: it is produced below the 32ºC and at higher temperatures it is turned off.<br>
-
For more details you can see the circuit:<a href="https://2013hs.igem.org/Team:CIDEB-UANL_Mexico/Project-Circuit" >Main Idea Description of the project.</a>
+
For more details you can see the circuit:<a href="https://2013hs.igem.org/Team:CIDEB-UANL_Mexico/Project-Idea" >Main Idea Description of the project.</a>
<center><img src="https://static.igem.org/mediawiki/2013hs/3/37/Circuito2L.jpg" width="700px" height="300px" /></center> </p>
<center><img src="https://static.igem.org/mediawiki/2013hs/3/37/Circuito2L.jpg" width="700px" height="300px" /></center> </p>

Revision as of 22:18, 20 June 2013

Project
Circuit

Circuit

The circuit works as the following way.
1. A pR (repressible promoter) is active and produces the Vip3Ca3 protein and the Green Fluorescent Protein as a reporter of the Vip3Ca3 production.
2. In the other part, there is a Constitutive promoter (pConst) but it works with a riboswitch.
3. The riboswitch is activated after the 32ºC and it starts trasnlation producing the cI protein which inhibits the pR turning off the Vip3Ca3 production.
In summary: The Vip3Ca3 production is regulated by temperature: it is produced below the 32ºC and at higher temperatures it is turned off.
For more details you can see the circuit:Main Idea Description of the project.

cideb
cideb
Contact us! Follow us on twitter and facebook or send us a mail.
CIDEB UANL Team. Centro de Investigación y Desarrollo de Educación Bilingüe
facebooktwitterenvelope

Retrieved from "http://2013hs.igem.org/Team:CIDEB-UANL_Mexico/Project-Circuit"