"
Team:TPHS SanDiego/Project
From 2013hs.igem.org
| Line 51: | Line 51: | ||
</p> | </p> | ||
<center> | <center> | ||
| - | <iframe src="https://docs.google.com/presentation/d/18V3cPgfWJIS7meO2SBBTGxSG1RrKxYvsg_Pxw9hB-2Q/embed?start=false&loop=false&delayms=3000" frameborder="0" width=" | + | <iframe src="https://docs.google.com/presentation/d/18V3cPgfWJIS7meO2SBBTGxSG1RrKxYvsg_Pxw9hB-2Q/embed?start=false&loop=false&delayms=3000" frameborder="0" width="1000" height="1109" allowfullscreen="true" mozallowfullscreen="true" webkitallowfullscreen="true"></iframe></center> |
<div class="row-end" ></div> | <div class="row-end" ></div> | ||
Revision as of 22:46, 20 June 2013
Our project focuses on promoter engineering. Our goal is to characterize a set of promoters (of our design) by moving the repressor and/or activator binding sites with respect to the -10 and -35 regions of the promoter. Ideally, we would like to show that by moving an activator binding site it can become a repressor and that by moving a repressor binding site it may become either irrelevant to transcription rate or even boost it. We also want to see if there is a steep decline in repressor/activator function as the binding site move along the promoter or if it is a gradual/linear change. We believe this project could have application to genetic circuits by allowing a single protein to either activate or repress a promoter depending on where the binding sites are placed on the promoter.
