Team:The Agency Escondido

Team Description

The Agency is an organization of students, parents, faculty and mentors operating out of the Classical Academy High School of Escondido, CA. Our objective is to compete in challenging venues, and to foster the development of skills and character needed to each occasion. Our efforts are focused in areas of science, technology, engineering, and mathematics (STEM).

Project Description

Our project seeks to create two triads of transparent bacteria strains with complementary promoter production to emulate the game of "rock, paper, scissors". Each triad will correspond to a group: group A and group B. Each type of bacteria will produce a specific protein that will act as a promoter to one and only one other bacteria strain(rock strain, paper strain, and scissors strain). Each triad will have different reporter genes designating its champion color. When two bacteria strains are combined in an agar plate, each protein secreted by the victorious strain, when acting as a promoter, changes the color of the cells on the plate identifying the champion.

At the time of writing, the promoters to be used have not been selected, nor have the reporter genes been chosen.

Safety

Our team strongly believes in the time-honored saying,“An ounce of prevention is worth a pound of cure,” and this philosophy has guided our precautionary decisions into one of the safest, best-structured, and tidiest work environments our organization has assembled to date. With copious amounts of reagents and hazardous laboratory equipment required for our projects, we have created a web application in our central services platform called CASP to manage the material safety data sheets (MSDS) of the reagents and equipment so that that they can be stored and accessed on a moments notice. MSDSs are designed to raise awareness of these substances and forewarn our members of their heterogeneous and sometimes dangerous natures.

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