Team:Shenzhen SZMS

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You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have the following information on your wiki:
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<li>a team description</li>
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REMEMBER, keep all of your pages within your teams namespace. <br><span style="font-weight:normal; font-style:italic;">Example: 2013hs.igem.org/Team:Shenzhen_SZMS/Our_Pets</span>
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Shenzhen_SZMS_team.png|right|frame|Your team picture]]
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|-
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|align="center"|[[Team:Shenzhen_SZMS | Team Shenzhen_SZMS]]
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===Team===
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== Project ==
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===Project Discription===
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Nitrate (NO3-) and nitrite (NO2-) are chemical roots of nitric acid (HNO3) and nitrous acid (HNO2) respectively. They exist in the aquatic environment, the living organisms and artificial products, as pollutants, which can cause food poisoning, cancers or even death.. So it's important  to detect and eliminate such chemicals with the efficient methods.. Several detection methods have already been in use but all are not entirely harmless. For example, granular Cd grains are easy to make and recover, and can be used repeatedly, so this procedure is recommended for the NO3- determination for fruits and vegetables. But Cd cannot be recycled easily, and may cause additional pollution. So we strived to design biosensors to avoid the disadvantages of traditional methods.
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    Our team is to develop novel modular biosensors that enable cost-effective and on-site detection of nitrates/nitrites by using an Escherichia coli chassis. And our development toward the nitrates/nitrites sensor is based on the previous work of the UT Dallas 2010 and Cambridge 2009. Since their aim is to enable E.coli to mark the existence of nitrates/nitrites with the GFP reporter, we found several drawbacks of this biosensor. Initially, the GFP reporter among the bacteria is not efficient enough to mark the inducer. It will only produce those green fluorescent protein with gloomy green light. Also, the process has been formed without any Recycling measures toward the proteins. Because of these shortages, our team thus aimed to find a possible replacement in order to reinforced the biosensor.
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    First, we find the initial part of biosensor, which is the PyeaR promoter that used to bind with nitrates and nitrites, from the work of previous teams. Then, to make a remarkable expression, we plan to insert a reporter genes, the CrtEBI, that, when synthesized, will lead the E. coli to release a specific pigment, lycopene, as visible color to determine the existence of nitrates/nitrites. Simultaneously, we will add another reporter, the banana odor enzyme (ATF1) generator, which is capable to lead the production of an aroma of bananas with the presence of nitrates/nitrites. Also, if capable, we wish to build our new biobrick to trigger the release of a certain enzyme, which can produce lipoxygenase to help in the degradation of the lycopene.
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　　The goal of our project is to provide an efficient and a more environment friendly method of detecting nitrates/nitrites.
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[[File:Overview of the biobricks.jpg]]
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===Notebook===
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Show us how you spent your days.
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===Results/Conclusions===
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===Attributions===
 
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Who worked on what?
 
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===Human Practices===
 
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What impact does/will your project have on the public? 
 
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===Fun!===
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</p><header>
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What was your favorite team snack?? Have a picture of your team mascot?
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 +
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 +
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 +
 
 +
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 +
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 +
 
 +
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 +
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 +
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 +
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 +
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 +
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      <table width="100%" height="107" border="0" cellpadding="0" cellspacing="0">
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          <td width="20">&nbsp;</td>
 +
          <td width="472"><span class="STYLE6">N</span><span class="STYLE1">itrate (NO3-) and nitrite (NO2-) are chemical roots of nitric acid (HNO3) and nitrous acid (HNO2) respectively. They exist in the aquatic environment, the living organisms and artificial products, as pollutants, which can cause food poisoning, cancers or even death. It's important to detect and eliminate such chemicals with the efficient methods. Currently, several detection methods have already been in use but all are not entirely harmless. For example, granular Cd grains are recommended for the NO3- determination for fruits and vegetables because they are easy to make and recover, and can be used repeatedly. However, Cd cannot be recycled easily, and may cause additional pollution. So the goal of our project is to provide an efficient and a more environment friendly method of detecting nitrates/nitrites and we strive to design biosensors to avoid the disadvantages of traditional methods. </span>           
 +
          <p class="STYLE1">Our team is going to develop novel modular biosensors that enable cost-effective and on-site detection of nitrates/nitrites by using an Escherichia</p></td>
 +
          <td width="20">&nbsp;</td>
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 +
 
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 +
<a class="bbox1 iconbox" >
 +
<div  align="center"  style="color:#CF900F;background:969696;height:35px;width:126px ">
 +
<b>OVERVIEW</b>
 +
</div>
 +
</a>
 +
 +
<a class="bbox2 iconbox" >
 +
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<b>OUTREACH</b>
 +
</div>
 +
</a>
 +
<a class="bbox3 iconbox" >
 +
<div  align="center"  style="color:#CF900F;background:969696;height:35px;width:126px ">
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<b>PICTURE</b>
 +
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 +
</a>
 +
            </div>
 +
 +
</div>
 +
<!--project--end-->
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 +
 
 +
    <!--文字--start-->
 +
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 +
<div id="boxinfo_default" style="display: block;">
 +
<h2>Human Practices</h2>
 +
<p>In order to make people know more about synthetic biology and IGEM,we use extracurricular to publicity our competition project.We also organize some activity to let people know it............</p>
 +
</div>
 +
<div class="orangebox" style="display: none;">
 +
<h2>Human Practices</h2>
 +
<p>In order to make people know more about synthetic biology and IGEM,we use extracurricular to publicity our competition project.We also organize some activity to let people know it............</p>
 +
</div>
 +
<div class="obox1" style="display: none;">
 +
<h2>Initiative</h2>
 +
<p>We took the initiative with the oil sands industry and established a dialogue between industry experts, academics, and government representatives. Through these talks a roadmap for the use of synthetic biology in the oil sands was established, focusing on biosensing and bioremediation.</p>
 +
</div>
 +
<div class="obox2" style="display: none;">
 +
<h2>Interviews</h2>
 +
<p>As an undergraduate team, we spoke with experts in various fields, including the oil industry, tailings pond management, biotechnology, law, and politics to gather various opinions on our project. How useful is synthetic biology in a tailings pond environment? What design considerations should we include in our project to improve security? What legal policies must we consider before implementing our project?</p>
 +
</div>
 +
<div class="obox3" style="display: none;">
 +
<h2>Design Considerations</h2>
 +
<p>Since safety is the driving force behind our project, we need to ensure the physical design of our biosensor and bioreactor would contain and manage the bacteria to minimize the possibility of their escape. We needed to build devices that would ensure the safety of both the user and the outside environment during their use. Click here to learn more!</p>
 +
</div>
 +
<div class="obox4" style="display: none;">
 +
<h2>Killswitch</h2>
 +
<p>Both FRED and OSCAR are designed to operate within enclosed environments. However, since safety is our highest priority, we decided to design and implement a killswitch in both FRED and OSCAR as an extra layer of security. The killswitch aims to destroy the genome using two powerful non-specific nucleases in the unlikely event that bacteria escape into the environment. Click here to learn more!</p>
 +
</div>
 +
<div class="obox5" style="display: none;">
 +
<h2>Safety</h2>
 +
<p>Click here for our safety page! Here we detail all the safety procedures, certifications, and approvals we have from our University to allow us to work this summer.</p>
 +
</div>
 +
<div class="obox6" style="display: none;">
 +
<h2>Community Outreach</h2>
 +
<p>iGEM Calgary partnered with a number of different associations to engage the general public about science and synthetic biology. Click here to see what we've done this summer!</p>
 +
</div>
 +
<div class="greenbox" style="display: none;">
 +
<h2>TEAM</h2>
 +
<p> It contains the team member and our directive teacher.Also our school’s introduction and the method of contacting us..................</p>
 +
</div>
 +
<div class="gbox1" style="display: none;">
 +
<h2>Detecting</h2>
 +
<p>FRED is our star detective, working around the clock to detect toxins roaming freely in tailings pond water. FRED is known for being a little unorthodox in his methods namely by using a measurable electrochemical signal rather than colored or fluorescent reporters. Using his transposon library FRED gets clues about the genetic elements that will activate in the presence of toxins. </p>
 +
</div>
 +
<div class="gbox2" style="display: none;">
 +
<h2>Reporting</h2>
 +
<p>Once FRED finds the toxins he wastes no time exposing them. Building upon last year's single output electrochemical system FRED now uses the new triple output system to report the toxins. This novel approach to electrochemical reporting means that FRED is as good as he is fast. </p>
 +
</div>
 +
<div class="gbox3" style="display: none;">
 +
<h2>Modelling</h2>
 +
<p>Aside from being a stellar detective FRED also dabbles in the art of mathematics and modelling. These skills are used to model the behavior of the system. The results from the modelling helped guide the wet lab experiments which in turn gave new data to refine the model.</p>
 +
</div>
 +
<div class="gbox4" style="display: none;">
 +
<h2>Prototyping</h2>
 +
<p>FRED gets a lot of information so he stays on top of things with the help of his handy dandy physical device to test the sample and software to interpret the raw data. The device has been <b>prototyped</b> and has an accompanying software platform that works with FRED to detect the toxins. </p>
 +
</div>
 +
<div class="bluebox" style="display: none;">
 +
    <h2>OTHER</h2>
 +
<p>There are the other part of project,including supporter and safety questions.........</p>
 +
</div>
 +
<div class="bbox1" style="display: none;">
 +
<h2>Overview</h2>
 +
<p>Our team is going to develop novel modular biosensors that enable cost-effective and on-site detection of nitrates/nitrites by using an Escherichia coli (E.coli) chassis. To introduce the whole process of our project, we split our works into 3 parts......</p>
 +
</div>
 +
<div class="bbox2" style="display: none;">
 +
<h2>Outreach</h2>
 +
<p><img src="https://static.igem.org/mediawiki/2013hs/0/09/Outreach_pic.jpg" /></p>
 +
</div>
 +
<div class="bbox3" style="display: none;">
 +
<h2>PICTURE</h2>
 +
<p><img src="https://static.igem.org/mediawiki/2013hs/3/39/Picture_content.jpg" /></p>
 +
</div>
 +
<div class="bbox4" style="display: none;">
 +
<h2>Bioreactor</h2>
 +
<p>OSCAR needed a home, so we developed an enclosed bioreactor system where toxins can be converted into hydrocarbons for output. Click here to see how we designed the bioreactor!</p>
 +
</div>
 +
<div class="bbox5" style="display: none;">
 +
<h2>Upgrading</h2>
 +
<p>Nitrogen and sulfur heteroatoms can produce nasty airborne pollutants when burned, cause acid rain and acid deposition, and can damage valuable catalysis mechanisms involved in fuel processing and emissions control. OSCAR can remove these atoms trapped in the rings. Click here to see how!</p>
 +
</div>
 +
</div>
 +
 +
<!--文字--end-->
 +
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 +
 
 +
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Latest revision as of 19:11, 21 June 2013

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  Nitrate (NO3-) and nitrite (NO2-) are chemical roots of nitric acid (HNO3) and nitrous acid (HNO2) respectively. They exist in the aquatic environment, the living organisms and artificial products, as pollutants, which can cause food poisoning, cancers or even death. It's important to detect and eliminate such chemicals with the efficient methods. Currently, several detection methods have already been in use but all are not entirely harmless. For example, granular Cd grains are recommended for the NO3- determination for fruits and vegetables because they are easy to make and recover, and can be used repeatedly. However, Cd cannot be recycled easily, and may cause additional pollution. So the goal of our project is to provide an efficient and a more environment friendly method of detecting nitrates/nitrites and we strive to design biosensors to avoid the disadvantages of traditional methods.

Our team is going to develop novel modular biosensors that enable cost-effective and on-site detection of nitrates/nitrites by using an Escherichia

  

 

Human Practices

In order to make people know more about synthetic biology and IGEM,we use extracurricular to publicity our competition project.We also organize some activity to let people know it............


Retrieved from "http://2013hs.igem.org/Team:Shenzhen_SZMS"