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Beril Gürdap
Name: Beril Surname: Gürdap
Age: 15
Hometown: Bolu, Turkey
Purpose in joining iGEM: Boredom
Job of your dreams: Psychologist
Plan for future: To be a great mum
Favourite Quote: I can do it
Favourite Music: Pop
Favourite Movie: Avatar
Country where you want to go: Great Britain because I've never been there
Personal Description: Hey guys! It’s not so hard to describe myself. One word: Galatasaray(Sports Club)!
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Team Members
If you want to learn more about us, just click the names!
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Büşra Güleçer
Name: Büşra Surname: Güleçer
Age: 16
Hometown: Elazığ, Turkey
Purpose in joining iGEM: To gain an experience about synthetic biology, designing a website and meeting with students like me from different countries
Job of your dreams: I want to be a doctor. Particularly, a heart surgeon
Plan for future: I am not sure, but after being a doctor, I may be an academician in a university and that moment I may treat poor people
Favourite Quote: Work for this world like you never die, work for other world like you will die tomorrow (Hz. Mohammad)
Favourite Music: Pop, R&B, verbal jazz, blues, and some of slow or classic songs
Favourite Movie: Millionaire
Country where you want to go: Countries like Haiti or Zimbabwe which are very poor. Maybe Palestine as there is violence. I live in good conditions. I should see them, and be grateful to God.
Personal Description: I like learning new things. So, I read, watch documentary. I like reading magazines about history, science. Also, I like recognizing other cultures, languages and religions. Especially, I read books about Islam and learn French and Arabic except for English. I am interested in NBA, but I don't follow matches,now. My favorite team is Boston Celtics and player is Rajon Rondo. I like doing tezhip( kind of Turkish art).
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Dilara Soylu
Name: Dilara Surname: Soylu
Age: 16
Hometown: Kayseri, Turkey
Purpose in joining iGEM: iGEM is a great way of understanding synbio world which is quite similar to the language of computers.
Job of your dreams: Computer engineer, physicist
Plan for future: to be helpfull to society/ to be happy/ to read 10.000 thick books
Favourite Quote: The best way to make dreams true, is to wakeup
Favourite Music: Classical music, Alternative Rock
Favourite Movie: V for Vandetta
Country where you want to go:Italy, it would be amazing to see one of the first center of science and philosophy
Personal Description: Someone trying to find out the meaning of "true", others can say waste of time but loves every issue about music, want to live in a world that everyone can pick out what to learn; without any pressure, wishing to be a masterCoder on her own, this is all how I describe myself.
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Mariye Erol
Name: Mariye Surname: Erol
Age: 16
Hometown: Yozgat, Turkey
Purpose in joining iGEM: iGEM is the way to gain an experience about synbio. Also to meet students like me from different countries
Job of your dreams: Surgeon, biotechnologist
Plan for future: I want to travel whole world
Favourite Quote: People of tomorrow shouldn’t linger with today
Favourite Music: Classical music, Pop
Favourite Movie: Dead Poets Society
Country where you want to go:It doesn’t matter for me because I would love to meet different kind of people, travel through different places
Personal Description: Hey guys, what up? Let me talk about myself. I love painting and I am interested in the calligraphy. I like playing table tennis and also playing the flute. I’m not Professional about this but it is a good way to spend my free time. Whatever sleeping is the best way to spend free time. :) that’s all :)
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Sinem Fırat
Name: Sinem Surname: Fırat
Age: 15
Hometown: Malatya, Turkey
Job of your dreams: General Surgeon
Plan for future: to be a normal person
Favourite Quote: People don't change
Favourite Music: Classical music, Pop
Favourite Movie: Harry Potter
Country where you want to go:It is Australia because I like Aussie people
Personal Description: Hey guys this is Sinem!! I don't know what am I gonna say but I'll try :) I really love playing basketball and volleyball. Also I like listening to music and some people say that I can sing :)I think I'm a cheerful person but sometimes I fell weird and I don't know why :) I love pandas and koalas, they make me feel happy. I think living your life is a great thing. All I wanted to say is, I am living my life so this is your life and just try to be happy :)
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Hatice Kurşun
Name: Hatice Surname: Kurşun
Age: 16
Hometown: Mersin, Turkey
Purpose in joining iGEM: To add knowledge to my knowledge
Plan for future: To be a beneficial person to society
Favourite Quote: In order to succeed, your desire for success should be greater than your fear of failure (Bill Cosby)
Favourite Music: Classical, Slow music
Favourite Movie: Inception
Country where you want to go:India, because you have a facility to meet people from variously different countries
Personal Description:Hi I'm Hatice. I'm glad you to meet you. I like meeting different people. I like swimming, travelling to different places. I'm interested in music, I like playing the guitar. I can't live without a sea but unfortunately I live in Ankara and there isn't any sea in here. I'm a trustworthy, cheerful and a little bit shy person. How about you people?
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Ayşe Demirci
Name: Ayşe Surname: Demirci
Age: 23
Hometown: Kastamonu, Turkey
Purpose in joining iGEM: iGEM is the port to the science world for me. I participated last year for the first time at the Collegiate Division and it was great! Now I want to assist NGSS Turkey by sharing my experiences about iGEM
Job of your dreams: A neurologist (for now :- ))
Plan for future: To be a good doctor and scientist /make a contribution to the understanding of the miraculous human body
Favourite Quote: Be the change that you wish to see in the world (Mahatma Gandhi)
Favourite Music: Actually I like EVERY kind of music;-) it makes no difference. I'm happy to be able to listen to just the right music I need for the mood I am in...
Favourite Movie: 3 Idiots
Country where you want to go:: Ireland. The nature of Ireland fascinates me since my childhood
Hobbies: Playing the violin, the guitar and the Ney (a Turkish reed flute). Basketball and football
Personal Description: I was born and grown up in Germany. Since 2011 I'm studying medicine at Turgut Ozal University. I'm a passionate violinist and I like almost all kinds of music. I joined the iGEM family last year at the collegiate division. It is a pleasure for me to advise NGSS Turkey. Thank you for the great time we had together:)
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Fatma Betül Çevik
Name: Fatma Betül Surname: Çevik
Age: 20
Hometown: Tokat, Turkey
Plan for future:to be a physician scientist
Favourite Music: Country, Classical
Favourite Movie: Hugo Cabret
Country where you want to go:: Japan, I want to observe and learn their culture
Personal Description: I’ll be third year medical student. I like my lessons without exceptions. I get excited by learning new things, especially in branches of science. I guess that’s why I take so much pleasure of iGEM studies (researches, experiments etc.). Furthermore, I think youths will achieve such incredible points in SynBio and in science as long as they are encouraged. I will try to be together with those young scientists which work with endless desire in every opportunity.
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Team Overview
Hi there,
Our team consists of 6 high school, 3 university students and 2 instructors. We are all so excited about iGEM HS 2013. We were ordinary people, who were just dealing with daily issues. Thank to SynBio and iGEM for giving such chance to us. Below you can find our team video prepared for iGEM 2013. "iGEM" brings us together"
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Adventures of E.coli and B.subtilis
Hi Dear iGEMers and the others,
Have you met E.Coli and B.Subtilis? Yes, these are the cute little bacteria that you are working on them at laboratory. They are small but smart bacteria. They are trying to be heard by humankind on twitter. Not just that, they are also instruct us about iGEM and synthetic biology. Of course they are doing it with their own interpretation.
Beside to be so active like this, both of the bacteria have different characteristics although they get along with each other. Let's get to know them closer:
E.Coli: Born on 1885. He is the unique king of the Eubacteria Kingdom. He is friend with B.Subtilis over a hundred year. He loves living in large intestine most. On the other hand, he wouldn't say no for a chocolate cake. His lifetime wish is to be the king of the world -or the universe with his words- when the day come that evolution has been completed.
B.Subtilis: Born on 1835. He has a modest lifestyle. E.Coli is his best friend-maybe because of he is his only friend. He hates antibiotics. Quiet places is his best. He prefers lemonade to chocolate His lifetime wish is to go to Pollyanna's village and continiue to his modest life. Although he is modest he says "When the world orders have changed, They will show the respect that I deserve!"
If you wanna learn more about E.coli and B.subtilis, now visit and follow their twitter addresses!
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Information Stand at Mall
As a human practises work, we were trying find out a influential way to inform the society about iGEM and SynBio. We decided on the group that would talk to. Public awareness was a serious issue, therefore we claimed the public as our first group.
Subsequently, we debated about how to inform the public effectively. We found out some efficient ways, and epening a stand in a shoppingmall was one of them. We choosed Nata Vega as a place, because it is the one of the biggest shoppingmall in Turkey. It also has famous brands which are only exist in there.
On 6 April 2013, our group has moved to the mall. We managed to complete our stand on 10.00 o’clock when the visitors has just reached. Our school has assists us with the bus to carry our materials. Days before, we had have sent our brochures for distrubuted the shops under the roof of NataVega.
We were ready with our balloons, brochures and ofcourse knowledge of SynBio, iGEM. We did estemeed number of presentations. Balloons were great idea to attractc people to our stand. We talked about what is SynBio and iGEM, example projects. Busra had borrowed her uncle’s camera to take professionals photos.
We did an attribution to be able to be better. Mariye and Dilara have distrubuted booklets and informed the people who visited our stand. Busra have taken photos. Beril and Sinem have distrubuted the balloons, booklets and also invited people to our stand. Most of the visitors were families, except that business men and student groups visited our stand as well.
After six hours, we have finished our presentations. It was a hard experience for us coming back with those equipments, they were really heavy, but it worth. We met people who can help us in our future projects, too. We met people who can help us in our future projects, too.
Here are the original brochures that we distrubuted on the mall (You can see the images with full size by clicking the small images)
Here are the English version of out brochures
Below you can find some photos from our mall event
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bactUNO Card Game
Hi there,
Our team consists of 6 high school, 3 university students and 2 instructors. We are all so excited about iGEM HS 2013. We were ordinary people, who were just dealing with daily issues. Thank to SynBio and iGEM for giving such chance to us. Below you can find our team video prepared for iGEM 2013. "iGEM" brings us together"
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SynBio Day at Kindergarden
We organized a SynBio day at the kindergarden. Our purpose was teaching new, different areas to kids and endearing science. We also aimed to measure their understanding of bacteria. First idea has came with the question :
"SynBio was a science area on university level. Although İgemhs competition showed us even high school students can understand and learn specific knowledge from SynBio. Why don’t we inform kindergarden students with the amount and level of information they can be able to understand?"
Those kids going to be futures astronauts, policemen, doctors, teachers etc. Does anyone ask how properly they know being an astronaut? So they can understand what we do, what bacteria are. We contacted with the teachers in Burc Child Academies, and explained our purpose. They were tend to do these kind of activities, so they let us to spend some good time with our little friends :)
On the first Kindergarden SynBio Day there was a group of kids aged between the range 3 – 6. Hatice Kursun, Sinem Fırat and Busra Gulecer went to Burc Child Academies, on 7th June 2013, at 10.30. Our friends prepared a great presentation on Powerpoint which is really colorfull and attractive. Presentation included brilliant pictures about bacteria. They also prepared a sketch, consisting of two beneficial hand made cartoon bacteria, to make children understand better.
Here are the notable questions that our friends asked to kids before the presentation and kids’ reaction to the questions:
Have you ever heard the word bacteria?
- Most of the kids have had heard about it.
Do you know that which functions bacteria have?
- Our friends got such answers: they sicken us, harm our teeth. The word bacteria didn’t not have a good impression on them.
Our friends have informed them with their presentation. They showed kids that there are some beneficial bacteria as well, and they are useful. Finally kids decided to pick our cartoon bacteria as friends :)
The first Kindergarden SynBio day passed great. Second Kindergarden SynBio day will come with surprises !!!
Below you can find our photos
Below you can find our powerpoint slide
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Survey
Our purpose in conducting this survey was to observe if people have enough information about synthetic biology, bacteria and activities which are related to them, or not. We surveyed with over 100 people. Most of them are high school students. The survey consist of 10 questions with 3 answer options.
You can find our survey in Turkish
You can find our survey in English
Question - 1
Are all kinds of bacteria harmful?
98% of the participants think that not all kinds of bacteria are harmful and say that some bacteria are even useful. It is suprising.
Question - 2
Do you have enough information about bacteria?
Most of participant haven’t got enough information about bacteria
Question - 3
Might bacteria be useful for the humanity?
97% of the people we have asked answered "yes" to this question.
Question - 4
Do you think that transferring genes to bacteria in order to gain various useful functions is justifiable?
For %38 of participants it is not justifiable. We need to inform people more about SynBio and its works.
Question - 5
Do you know which functions bacteria have/might have (except for illness)?
That is a good ratio. Still there are some people don’t know about bacteria’s function
Question - 6
Do you think that changing genes is acceptable?
Most of the participants think that transferring genes is ok, but changing genes on the other hand is not right.
Question - 7
Have you ever heard of E.Coli?
%55 of the participants have not heard about E.coli
Question - 8
Might be bacteria enjoying?
Most of them said that bacterias might not be enjoying.
Question - 9
Do you think that you have enough knowledge about synthetic biology?
60% of the people noticed that they don’t know enough about SynBio.
Question - 10
Do you follow competitions and activities about Synthetic Biology?
82% of the pople don’t know about activities related to bacteria/SynBio like iGEM.
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SynBio Presentation
As 2013 iGEM HS participants, NGSS TURKEY, we made an Introduction to Primary School Students. On 15th of May, we invited them to our school and organized a well designed presentation. They were all senior year students at a primary school, and as they have a High School Entrance Examination (SBS), SynBio and iGEM presentation was like a breathe for them.
Beril Gurdap has presented and Busra Gulecer has taken the photos, and she also prepared the presentation. We gave place to important notes in Powerpoint, and prepared a presentation according to that. The presentation was consisting of brief descriptions and lasted for nearly 45 minutes. Beyond the PowerPoint presentation Beril also gave place to information about our team, NGSS TURKEY.
Firstly, we explained what SynBio is. We mention the differences and similarities between genetical engineering and SynBio. We measured how the audience know about SynBio. We proved again that SynBio is a young field of science.
As every slide included iGEM logo, we explained what the igem is, too We got th attention on this part. They asked about how to participate. We explained them in the way as good as we can. We picked up next years iGEMers :)
The heart of İgem, SynBio. Pipettesss!!! We could not afford one, so we could not show what it is like in real life, although our media was able to make sense. These magical tools... They are certainly amazing! After those reactions we have to explain to our visitors that they can not buy one for themselves and keep it. Actually they can, but without eppendorfs and plates what to do with pipettes?
We showed them a plate and explained the points on the plate. It is so hard to believe that on these little plates there are more than millions of bacteria. We continued with sample projects of previous years. They were impressed by the shined bacteria. When we talked about the power of this light, we gave reading a book in front of luminous bacteria as an example. We also talked about the future of SynBio and asked them what they want e.coli to do.
Seeing smiling faces made us feel good :)
Below you can find our presentations
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Introduction of SynBio to Companies
On SynBio projects, presentations of the projects are as important as what projects are. As synthetic biologists, beyond SynBio, as scientists our intention is to inform society about new technologies, about how to make the world a better place to live and to increase the awareness of public. Society sometimes refers kids or students and sometimes people in Supermarkets. There is also one more group of people. They are businessmen. Gaining the attention of those people has slightly different benefits to SynBio Society.
Our purposes on SynBio and İgem introductions to companies have similarities with our other presentations. We are trying to inform those people about the area that we are working on. Almost ;) in every attendance we take good feedbacks. People like to hear new things, especially from youngsters. Their supports are considerable for us. With their encouragement we hope one day we are going to be able to be beneficial for humanbeings.
To present SynBio and İgem we have contacted with several companies. After couple of struggles we managed to start a communication with some of them. We prepared a stable presentation including SynBio, iGEM, Example Projects and Our Team.
Energy Market Regulatory Authory
We have contacted with Mr. Osman Birgin, who is the head of Energy Market Regulatory Authory, he was enthusiastic for such kind of activities, so he has accepted our offer. We have taken appointment from him for 5th of June 2013 , at 11.00 am. Mariye Erol and Dilara Soylu as the students and Fatma Betül Çevik as the advisor have gone for meeting. When we arrived, we had a conversation in Mr. Birkan’s room. Afterward we passed to meeting room and started our presentation. The presenatation has been done to Mr.Birgin, other department presidents and a septet of EMRA workers.
The audience has listened the presentation of little-known discipline synthetic biology and iGEM competition carefully. We’ve informed them about the studies on İgem related to energy. Our friends have answered the questions. Then we talked about the future of SynBio. Mr. Birkan offered us sending our presentation documents as an email to the ones on their email lists who are almost five hundred people.
We informed them about our outreach projects. After couple of time, Mr. Birkan offered to us sending our presentation document as email to the ones on their email list who are almost five hundred people (Document was helpful :)) We have left EMRA about 12.40 am. Beyond being able to reach our purposes, we enjoyed to spend time with them!
Halıcızade Company
From our team; Mariye Erol, Dilara Soylu and Fatma Betül Çevik have established communication with the founder and the head of Halicızade Company, Mr. Metin Özer. We have talked about whether we can make a presentation or not Mr. Ozer has accepted our offer.
The presentation has been placed on 5 June 2013, at 14.00. It has lasted for 30 minutes. Firstly we explained what SynBio and İgem is. Then we have showed the works of previous teams. After we completed the presentation, they spent some time on possible kinds of projects that can be done in the area of SynBio. It was a beneficial presentation for us and him, Mr. Ozer has given his thanks. Afterward we left him our presentation and sponsorhip document and left Halıcızade Company at 14.00.
Below you can find some photos from the our presentations
FIRAT COMPANY
Mr. Fırat has accepted our presentation offer kindly. On 18 May, at 10.00 a.m. Sinem and Hatice from our team went to Fırat Company for the presentation. Audience consisted of six person. We used the powerpoint slides to inform the listeners. After sincere conversation we left the company. It was a succesfull presentation, we also developed our presentation skills.
Below you can find some photos from the our presentations
Here are our presentation documents :
Click here for our powerpoint slide in Turkish
Click here for our powerpoint slide in English
Click here for our sponsorship and presentation document in Turkish
Click here for our sponsorship and presentation document in English
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iGEM for Everyone
The time that we have spent in public transportations, most of the time, is wasted time. Imagine that you are in the subway, in the bus, walking or shopping; group of people came out started to tell you about such intersting issues. Wouldn’t it great? Today you learn something new dude!
Why am I telling that? We are also part of the mass. In order to arouse the interest We printed some cute brochures - OK they were well designed but we couldn’t print them all in colours(By the way, we also used the last brochures which were left over from the information stand in the shopping mall ). It was our goal to make iGEM understandable for every member of the society.
Where have we made our presentations ? Subway, bus, shopping mall, street, picnic area – everywhere we could see any human beeing! If we had a sea or a river in the neighborhood ships couldn’t escape us either. We informed people about SynBio and iGEM by leaving out the technical language out as far as possible, so even children could understand us.
We had very positive feedback and especially elderly persons stated that they are very proud of us to take part in scientific projects like iGEM at such a young age.
We also have some funny moments (especially in the subway). It was a really nice experience!
Below you can find our brochures
For English
For Turkish
Below you can find our photos
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Fun!
One day of an iGEMer: "Experiment, wiki, experiment, wiki, experiment, wiki... zZzZzZz..."
Listening Ayşe Sister's guitar was a great pleasure for us. Also Dilara can play, but... In our free times, it is not valid actually, we played table tennis or took videos. The best parts of the days, definitely, meal times. Beril's mother bought us a baklava (turkish dessert, yummy!) for once :) Our traditional meal is pizza. Ordering pizza is cheaper than the others. We can choose "Secrets" as our favourite team song. Our favourite sport is wrestling, also.After three weeks that we stayed in the lab, we have collected some funny moments. Beyond them we took some videos else too. Enjoy it!"
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Lab
Notebook
Here is the Notebook since January!
Procedures
If you want to learn how we did our experiments, you are in the right place
Safety
Safety have never been such funny!
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Outreach
Adventures of E.coli and B.subtilis
Have you met E.Coli & B.Subtilis? ...
InfoStand at Mall
As a human practises work, we ...
bactUNO card game
As a human practises work, we ...
SynBio Day at the Kindergarden
We organized a SynBio day @kindergarden ...
Survey
As a human practises work, we ...
SynBio Presentation
As a human practises work, we ...
Introduction of SynBio to Companies
On SynBio projects, presentations ...
iGEM for Everyone
As a human practises work, we ...
NGSS Comics
As a human practises work, we ...
Colloborations
As a human practises work, we ...
Digestion
Materials:
- • Pcr Tube
- • Pipette tip which has filter
- • Plasmid which had isolated
- • -20 C EcoR1, Spel, Pstl, Xbal enzymes
- • ×10 NE Buffer 2
- • ×100 BSA
- • Nuclease Free H2O
- • Heat block or PCR
Experiment:
- • Take PCR tubes. Don’t forget to take tube for destination plasmid.
- • Paste the stickers to the tubes.
- • Lave NE Buffer 2, NF Water, BSA into ice when they melted.
- • Take the average of the nucleic acid concentrations measured by the spectrometer. Divide 500 by the DNA average.
- • Add 5µl Ne Buffer.
- • Add 0.5µl BSA Buffer.
- • Add 1 µl of the enzymes with barrier tips.
- • If you cut with Ecor1 and SpI, it will be up stream.
- • If you cut with Xbal and Pst1, it will be down stream.
- • Subtract the amount of DNA from 42.5 µl. This result will be the amount of NFW used.
- • Add the NFW with barrier tips and do one pippetting while taking the NFW.
- • Then the DNA is put into the PCR and is left there for 30 minutes.
ATTENTION!
- • Be sure enzymes are enough.
- • You must use plasmid which had isolated.
- • Use barrier tips has filter.
Plasmid Isolation
Materials:
- • Centrifuge
- • Liquid waste bin
- • 1,5 ml and 2 ml epps
- • Plasmid Isolation Kit
- • Resuspension Buffer
- • Lysis Buffer
- • Neutralization Buffer
- • Wash Buffer
- • Elution Buffer
- • Vortex
- • 50-500 µl pipettes
- • Timer
- • Filter columns and epps
- • PCR Tubes (mini epp)
Experiment:
- • Use gloves!
- • Liquid cultures centrifuge: 5 ml; 4100 rpm, 15 min. 1,5 ml; 13000 rpm, 10 min. (with 2 µl epp)
- • After the centrifuge, the supernatent should be disposed without taking any pellets along with it.
- • Add 250 µl Resuspension Solution. Vortex until to be homogeneous(about 30 sec.). You can pipetting too.
- • Add 250 µl Lysis Solution. You must invert(4-6 times).
- • There must be 3 min.
- • Add 350 µl Neutralization Solution and invert(4-6 times).
- • 5 min. centrifuge.
- • After centrifuge try to take supernatent without pellets as much as you can to fitler columns.
- • 1 min. (13 000 rpm) centrifuge. Throw the supernatent to liquid waste bin.
- • Add 500 µl Wash Solution. 1 min. centrifuge. Throw the supernatent to liquid waste bin.
- • Add 500 µl Wash Solution. 1 min. centrifuge. Throw the supernatent to liquid waste bin.
- • 1 min. centrifuge to get rid of alcohol.
- • Throw the supernatent with epp.
- • Put new 1,5 epp under the filter column.
- • Transfer the columns into 1.5 ml microcentrifuge tubes and leave their caps open for 2 minutes so that the ethanol that they contain dissolves in air.
- • Add 50 µl Elution Buffer.
- • Incubate for 2 minutes.
- • Centrifuge for 2 minutes.
- • Take the supernatent to new epp.
- • Measure them with Nanodrop.
Attention!
- • All centrifuges are 13000 rpm.
- • Don’t shake Lysis Solution.
LB-Agar
Materials:
- •LB-Agar(chemical storage)
- •Sterile dH2O
- •Flask
- •Liquid autoclave
- •Aluminium folio
- •Antibiotic
- •Empty plates
- •Parafilm
Experiment:
- •In a steril environment, the tare of the container should be measured and subtracted from the overall weight.
- •7 grams of LB Agar is put in the container.(If there is no LB agar, add the 4 gram LB broth and 2,7 gram agar.)
- •Add 200 ml distilled water to graduated cyclindar.
- •These two are mixed in a beaker.
- •When you are opening the beaker be sure that it doesn’t contact with air.
- •Autoclave tape is sticked on to the aliminium.
- •The beaker is placed in to the autoclave machine.
- •Autoclave machine has to have distilled water or demineralized water. The water level in the autoclave machine has to be a little higher than the liquid level of the beaker.
- •Then start the 90 min.
- •Take out the beaker and add antibiotics if required.
Attention!
- •Don’t be far away fire.
- •Don’ forget to write the date.
- •Don't forget add the amtibiotics
LB-Broth
Materials:
- •LB-Broth(chemical storage)
- •Sterile dH2O
- •Flask
- •Lab skale
- •Liquid autoclave
- •Alumininum folio
- •Antibiotic
Experiment:
- •In a steril environment, the tare of the container should be measured and subtracted from the overall weight.
- •4 grams of LB Broth is put in the container.
- •Add 200 ml distilled water to graduated cyclindar.
- •These two are mixed in a beaker.
- •When you are opening the beaker be sure that it doesn’t contact with air.
- •Autoclave tape is sticked on to the aliminium.
- •The beaker is placed in to the autoclave machine.
- •Autoclave machine has to have distilled water or demineralized water. The water level in the autoclave machine has to be a little higher than the liquid level of the beaker.
- •Then start the 90 min.
- •Take out the beaker and add antibiotics if required.
Attention!
- • Don’t be far away fire.
- • Don’ forget to write date
Ligation
Materials:
- • PCR Tube
- • Plasmids
- • 10× T4 DNA Ligase Buffer
- • T4 DNA Ligase
- • Nuklease Free H2O
- • Heat Block
Experiment:
- • Take PCR Tube ® Write Lig on it
- • Add 2µl upstream, 2µl downstream, 2µl destination plasmids
- • Add 2µl T4 DNA Ligase buffer
- • Add 1µl T4 DNA Ligase
- • Add 11µl nüklease free H2O. It must be 20 µl.
- • It must wait 37 C (10 min.), 80⁰C(20 min.)
- • After incubation you must followwith transformation. The only difference between normal transformation and lig’s transformation is adding 2µl plasmid.
Attention!
- • You must leave plasmid in -20°C after lig.
- • If electrophoresis results are successful you will follow with lig.
Liquid Culture
Materials:
- • Falkon
- • Pipette gun
- • 5-10 ml pipette
- • Ice box
- • LB-Broth
- • Antibiotic
- • Colony
- • Pipette
- • Shaker
- • Incubator
Experiment:
- In sterile environment,
- • Take falkons.
- • Add 5 ml LB-Broth into falkons with pipette gun.
- • Take an antibiotic into ice.
- • Add antibiotic.
- • Take the colony from plate.
- • Leave it into falkon.
- • Take falkons to the shaker.(it can be 240 or 320 rpm) Leave it 37⁰C ( 12-14 hours ) incubator.
Nano Drop
- • Open the program(Nano- Spech).
- • Choose dsDNA.
- • Be sure pipette is 2 µl.
- • Leave the drop middle.
- • First try Elution Buffer (for blanck)
- • Click blank.
- • If results are about 0(zero) its okay.
- • Write their names, click "start".
- • Nucleic Acid must be much over than 17 ng.
- You must check 2 times and to take their avarage.
Notes:
- Blanck = Elution Buffer
- Graph must be as straight as it can.
Transformation
Materials:
Notes:
- • 1.5 mL epps
- • Heat Block (42ºC)
- • Competent cells
- • LB-Broth
- • Ice
- • Ice Box
- • Microcentrifuge
- • Rocks for epp
- • Timer
- • LB agar plates
- • Spreader
- • Shaker (240 rpm)
- • Parafilm
- • Band
- • 1 µl pipette (for plasmid)
- • 50µl pipette
- • 150 µl pipette (for spread)
- • 450 and 500 µl pipettes (for LB)
- • Incubator (37 C)
- • Alcohol
- • If there is diluting from the kit:
- • Nuclease Free H2O
- • 15 µl pipette
- • PCR Tube (Mini epp)
Experiment:
Notes:
- • Sterilize the environment.
- • Take the competent cells from -80°C.
- • Turn on the heat block (42⁰C)
- • Put 1 µl plasmid in a 1,5 ml ep.
- • Put competent cells in the same 1,5 ml epp. Use 50µl competent cells.
- • Place the epps into the microcentrifuge. (30-60 sec., 3000 rpm)
- • Incubate on ice for 45 min.
- • Put epps into the heat block at 42⁰C for 120 sec.
- • Put epps back on ice for 5 min. to reduce damage to the e.coli cells.
- • Add 450 ul of LB(with no antibiotic added)
- • Incubate examples on shaker for 1 hour at 37⁰C. (240 rpm)
- • Spread about 135 ul of the final examples on LB plates (with appropriate antibiotic added-usually Ampicillin or Kanamycin)
- • Incubate overnight.
- • Pick colonies about 14-16 hours later
ATTENTION
Notes:
- • Don’t use gloves when you are working with fire.
- • Don’t forget to write notes on epps.
- • Be quick while sirkini Wilhelm compatent cells
Attributions
Attributions
All of us work hard for iGEM 2013. But our particular thanks go to the esteemed people and foundations behind the scenes...
Firstly we want to thank our school, Ankara Private Atlantik Nevin Gökçek Science High School , to give us such opportunities.
Our very special thanks go to...
Wet Lab Works
...Turgut Ozal University (former Fatih) for providing their labs and other facilities for us. We also want to thank our head advisor Assoc. Prof. Esra Gündüz who was ready at anytime to support us.
...Sentegen for helping us to to provide our genes, special thanks for cool centrifuge.
...the whole team, especially to our advisors: Fatma Betül Çevik, Ayşe Demirci, Esma Ölmez who were always with us.
Wiki
... Ms. Havva Gulay Gurbuz – graduate student at Bilkent University. She spent a lot of her time for us.
... Busra Nur Aydın who helped us with designing - she has magical hands.:)
...Mr. Kasım Özkan who helped us with doing our comics.
Funding
We want to thank Mr. Osman Birgin , who is the head of Energy Market Regulatory Authory for finding us generous funds. He did things which mean a lot for us.
We want to thank our school again in this chapter. They provided their sources for us.
Beyond these, we want to thank Turgut Ozal 2013 Collegiate iGEMers for being our advisors. We also want to give a special thank to Esin and Kübra Sisters for yummy meals :)
There is also one more group that we want to thank. They were supporting us in our desperative times. Our parents! Especially to Beril’s mom Mr. Hülya Gürdap for baklava (Turkish dessert) and to Dilara’s mom Mr. Özlem Soylu for transportation.
Aaaand finally: Our instructor Ms. Mihriban Ozgun for her amazing "instructorhood"
Team:NGSS AEI TURKEY
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Revision as of 22:09, 21 June 2013