Team:TPHS SanDiego

From 2013hs.igem.org

(Difference between revisions)
 
(251 intermediate revisions not shown)
Line 1: Line 1:
-
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
+
<html xmlns="http://www.w3.org/1999/xhtml"
-
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
+
      xmlns:og="http://ogp.me/ns#"
-
<head>
+
      xmlns:fb="https://www.facebook.com/2008/fbml">
-
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
+
  <head>
-
<meta name="generator" content="MediaWiki 1.16.5" />
+
    <title>Berkeley iGEM 2011 </title>
-
<link rel="shortcut icon" href="/favicon.ico" />
+
    <meta property="og:title" content="Berkeley iGEM 2011"/>
-
<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2013hs.igem.org (en)" />
+
    <meta property="og:type" content="university"/>
-
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:TPHS_SanDiego&amp;action=creativecommons" rel="meta" />
+
    <meta property="og:description"
-
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" />
+
          content="Berkeley's 2011 iGEM team wiki."/>
-
<link rel="alternate" type="application/atom+xml" title="2013hs.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&amp;feed=atom" /> <title>Team:TPHS SanDiego - 2013hs.igem.org</title>
+
<meta name="description" content="Berkeley iGEM 2011" />
-
<style type="text/css" media="screen, projection">/*<![CDATA[*/
+
<meta name="keywords" content="igem, berkleley, 2011" />
-
@import "/wiki/skins/common/shared.css?270";
+
-
@import "/wiki/skins/igem/main.css?270";
+
-
background-color: #000;
+
-
/*]]>*/</style>
+
-
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" />
+
-
<!--[if lt IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE50Fixes.css?270";</style><![endif]-->
+
-
<!--[if IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE55Fixes.css?270";</style><![endif]-->
+
-
<!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?270";</style><![endif]-->
+
-
<!--[if IE 7]><style type="text/css">@import "/wiki/skins/igem/IE70Fixes.css?270";</style><![endif]-->
+
-
<!--[if lt IE 7]><script type="text/javascript" src="/wiki/skins/common/IEFixes.js?270"></script>
+
-
<meta http-equiv="imagetoolbar" content="no" /><![endif]-->
+
-
+
-
<script>
+
-
var skin="igem",
+
-
stylepath="/wiki/skins",
+
-
wgUrlProtocols="http\\:\\/\\/|https\\:\\/\\/|ftp\\:\\/\\/|irc\\:\\/\\/|gopher\\:\\/\\/|telnet\\:\\/\\/|nntp\\:\\/\\/|worldwind\\:\\/\\/|mailto\\:|news\\:|svn\\:\\/\\/",
+
-
wgArticlePath="/wiki/index.php/$1",
+
-
wgScriptPath="/wiki",
+
-
wgScriptExtension=".php",
+
-
wgScript="/wiki/index.php",
+
-
wgVariantArticlePath=false,
+
-
wgActionPaths={},
+
-
wgServer="https://2013hs.igem.org",
+
-
wgCanonicalNamespace="",
+
-
wgCanonicalSpecialPageName=false,
+
-
wgNamespaceNumber=0,
+
-
wgPageName="Team:TPHS_SanDiego",
+
-
wgTitle="Team:TPHS SanDiego",
+
-
wgAction="view",
+
-
wgArticleId=0,
+
-
wgIsArticle=true,
+
-
wgUserName=null,
+
-
wgUserGroups=null,
+
-
wgUserLanguage="en",
+
-
wgContentLanguage="en",
+
-
wgBreakFrames=false,
+
-
wgCurRevisionId=0,
+
-
wgVersion="1.16.5",
+
-
wgEnableAPI=true,
+
-
wgEnableWriteAPI=true,
+
-
wgSeparatorTransformTable=["", ""],
+
-
wgDigitTransformTable=["", ""],
+
-
wgMainPageTitle="Main Page",
+
-
wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2013hs.igem.org", "5": "2013hs.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"},
+
-
wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2013hs.igem.org": 4, "2013hs.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},
+
-
wgSiteName="2013hs.igem.org",
+
-
wgCategories=[],
+
-
wgMWSuggestTemplate="https://2013hs.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest",
+
-
wgDBname="2013hs_igem_org",
+
-
wgSearchNamespaces=[0],
+
-
wgMWSuggestMessages=["with suggestions", "no suggestions"],
+
-
wgRestrictionEdit=[],
+
-
wgRestrictionMove=[];
+
-
</script>               
+
-
<script type="text/javascript" src="/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script>
+
-
<!-- Head Scripts -->
+
-
<script src="/wiki/skins/common/ajax.js?270"></script>
+
-
<script src="/wiki/skins/common/mwsuggest.js?270"></script>
+
-
<script type="text/javascript" src="/wiki/index.php?title=-&amp;action=raw&amp;gen=js&amp;useskin=igem"><!-- site js --></script>
+
-
<!-- jQuery Javascript -->
+
-
<script type='text/javascript'        src ='/common/jquery-latest.min.js'></script>
+
-
<script type='text/javascript'        src ='/common/tablesorter/jquery.tablesorter.min.js'></script>
+
-
        <link rel='stylesheet' type='text/css' href='/common/tablesorter/themes/groupparts/style.css' />
+
-
        <link rel='stylesheet' type='text/css' href='/common/table_styles.css' />
+
-
        <link rel='stylesheet' type='text/css' href='/forum/forum_styles.css' />
+
-
<script type='text/javascript'        src ='/forum/forum_scripts.js'></script>
+
-
</head>
+
-
<body              class="mediawiki  ltr ns-0 ns-subject page-Team_TPHS_SanDiego">
+
<link rel="stylesheet" href="https://2011.igem.org/Template:Team:Berkeley/nivocss?action=raw&ctype=text/css" type="text/css"/>
-
  <div id="globalWrapper">
+
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.3.2/jquery.min.js"></script>
-
    <div id="top-section">
+
<script type="text/javascript" src="https://2011.igem.org/Template:Team:Berkeley/javascript/javanivoslider?action=raw&ctype=text/javascript"></script>
-
<div id="p-logo">
+
  </head>
-
    <a href="/wiki/index.php/Main_Page"
+
-
      title="Main Page"><img src="IGEM Title.png" width="1007" height="170" alt="IGEM Title" />"
+
-
    </a>
+
-
</div> 
+
-
<!-- end p-logo -->
+
-
<script type="text/javascript"> if (window.isMSIE55) fixalpha(); </script>
+
-
<div id="menubar" class='left-menu noprint'>
 
-
  <ul>
 
-
                  <li
 
-
                class='selected new'       ><a href="/wiki/index.php?title=Team:TPHS_SanDiego&amp;action=edit&amp;redlink=1">Page              </a></li>
 
-
              <li
 
-
                class='new'       ><a href="/wiki/index.php?title=Talk:Team:TPHS_SanDiego&amp;action=edit&amp;redlink=1">Discussion              </a></li>
 
-
              <li style='color:white;cursor:default'>teams</li>
 
-
  </ul>
 
-
</div> <!-- end menubar (left) -->
 
-
<div class="right-menu noprint" id="menubar">
 
-
    <ul>
 
-
                <li id="pt-login"><a href="/wiki/index.php?title=Special:UserLogin&amp;returnto=Team:TPHS_SanDiego" title="You are encouraged to log in; however, it is not mandatory [o]" accesskey="o">Log in</a></li>     </ul>
 
-
</div><!-- end right menubar -->
 
-
<div id="search-controls" class="noprint">
+
 
-
<form action="/wiki/index.php/Special:Search" id="searchform">
+
<div id="top-section3"><center><img src="https://static.igem.org/mediawiki/2013hs/3/35/TorrePinesNavigation%28Tareq%29.jpg" alt="header" border="0"  usemap="#igemmap" width="1000"></center></div>
-
<input id="searchInput" name="search" type="text" title="Search 2013hs.igem.org [f]" accesskey="f" value="" />
+
 
-
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;
+
<map name="igemmap">
-
      <input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" />
+
  <area  shape="circle" coords="480,40,40" alt="Mercury" href="https://2013hs.igem.org" onfocus="this.blur()" />
-
</form>
+
</map>
-
</div> <!-- close search controls -->
+
 
-
    </div> <!-- close top-section-->
+
 
-
    <div id="content">
+
<div class="layout-1000">
-
<a name="top" id="top"></a>
+
<div class="row">
-
<h1 class="firstHeading">Team:TPHS SanDiego</h1>
+
<div class="col12" >
-
<div id="bodyContent">
+
<ul class="topnav">
-
<h3 id="siteSub" class='noprint'>From 2013hs.igem.org</h3>
+
    <li><a href="https://2013hs.igem.org/Team:TPHS_SanDiego">Home  </a></li>
-
<div id="contentSub"></div>
+
    <li>
-
<!--
+
        <a href="https://2013hs.igem.org/Team:TPHS_SanDiego/Project">Project</a>
-
<div id="jump-to-nav">Jump to:                        <a href="#column-one">navigation</a>, <a href="#searchInput">search</a></div>-->
+
    </li>
-
<!-- start content -->
+
    <li>
-
<div class="noarticletext">
+
        <a href="https://2013hs.igem.org/Team:TPHS_SanDiego/Parts">Parts</a>
-
<p>There is currently no text in this page.
+
    </li>
-
You can <a href="/wiki/index.php/Special:Search/Team:TPHS_SanDiego" title="Special:Search/Team:TPHS SanDiego">search for this page title</a> in other pages,
+
    <li><a href="https://2013hs.igem.org/Team:TPHS_SanDiego/Safety">Safety</a></li>
-
or <span class="plainlinks"><a href="https://2013hs.igem.org/wiki/index.php?title=Special:Log&amp;page=Team:TPHS_SanDiego" class="external text" rel="nofollow">search the related logs</a></span>.
+
    <li><a href="https://2013hs.igem.org/Team:TPHS_SanDiego/Team">Team</a></li>
 +
    <li><a href="https://2013hs.igem.org/Team:TPHS_SanDiego/Protocols">Protocols</a></li>
 +
    <li><a href="https://2013hs.igem.org/Team:TPHS_SanDiego/Journal">Journal</a></li>
 +
</ul>
 +
<div> <div class ="row-end"> </div>
 +
</div>
 +
</div>
 +
 
 +
<div class="row">
 +
<div class="col12" id="spacer2"></div>
 +
<div class="row-end"> </div>
 +
</div>
 +
 
 +
 
 +
<div class="row">
 +
<div class="col6" align = "justify" >
 +
<img src="https://static.igem.org/mediawiki/2013hs/4/46/ABstractBar.jpg" width="480">
 +
<p style="font-family:Georgia;color:black;font-size:14px;">In an effort to expand the toolkit available to synthetic biologists, we've taken a system natively responsible for transcriptional activation and modified it to control transcriptional repression. The LasR system from <i>Pseudomonas Aeruginosa</i> requires the presence of a small molecule, C12-3-oxo-AHL, to induce activation of the Plas promoter. By modifying the -10 and -35 sites of the promoter, as well as shifting the location of the LasR binding sites, the new Plas promoter (Plas*) was changed from an inducible to a repressible promoter. Through adding this second functionality, the Plas* promoter could be used in conjunction with a wildtype Plas promoter to control two separate genes whose expression levels are always out of sync. Furthermore, if the bacteria are transfected with a plasmid encoding LasI, the bacteria will be able to turn off gene expression at a critical population density, instead of only being able to turn on gene expression.</p>
 +
 
 +
<div class ="row-end"> </div>
 +
</div>
 +
 
 +
<div class="row">
 +
<div class="col12" id="spacer"></div><center>
 +
<iframe width="460" height="345" src="http://www.youtube.com/embed/Ohy-y8M4j_M" frameborder="0" allowfullscreen></iframe></center>
 +
<div class="row-end"> </div>
 +
</div>
 +
 
 +
<div class="row">
 +
 
 +
<div class="col3-2"style="background-color:#101a4d;"><a href="https://2013hs.igem.org/Team:TPHS_SanDiego/Background#TOP"> <img src="https://static.igem.org/mediawiki/2013hs/8/86/Fluob4.png"
 +
onmouseover="this.src='https://static.igem.org/mediawiki/2013hs/f/f5/Fluoafter.png'"
 +
onmouseout="this.src='https://static.igem.org/mediawiki/2013hs/8/86/Fluob4.png'" width="230"></a>
 +
<p style="font-family:Georgia;text-align:center; color:#CECECE;"> Proteins discovered in Jellyfish that have the
 +
ability to emit fluorescence.</p> </div>
 +
 
 +
<div class="col3-2"style="background-color:#185f73;"><a href="https://2013hs.igem.org/Team:TPHS_SanDiego/Background#MIDDLE"><img src="https://static.igem.org/mediawiki/2013hs/2/2a/2pb4.png"
 +
onmouseover="this.src='https://static.igem.org/mediawiki/2013hs/1/1c/2pafter.png'"
 +
onmouseout="this.src='https://static.igem.org/mediawiki/2013hs/2/2a/2pb4.png'" width="230"> </a>
 +
<p style="font-family:Georgia;text-align:center; color:#CECECE;"> A clever way to normalize protein expression across cell populations.</p></div>
 +
 
 +
<div class="col3-2" style="background-color:#303285"><a href="https://2013hs.igem.org/Team:TPHS_SanDiego/Background#BOTTOM"><img src="https://static.igem.org/mediawiki/2013hs/5/5a/Lasrafter.png"
 +
onmouseover="this.src='https://static.igem.org/mediawiki/2013hs/a/aa/Lasrb4.png'"
 +
onmouseout="this.src='https://static.igem.org/mediawiki/2013hs/5/5a/Lasrafter.png'" width="230"></a>
 +
<p style="font-family:Georgia;text-align:center; color:#CECECE;"> A quorum sensing system from <i>Pseudomonas aeruginosa</i>.</p> </div>
 +
 
 +
<div class="col3-2"style="background-color:#499394"><a href="https://2013hs.igem.org/Team:TPHS_SanDiego/Project"><img src="https://static.igem.org/mediawiki/2013hs/5/54/Projb4.png"
 +
onmouseover="this.src='https://static.igem.org/mediawiki/2013hs/7/74/Projafter.png'"
 +
onmouseout="this.src='https://static.igem.org/mediawiki/2013hs/5/54/Projb4.png'" width="230"></a>
 +
<p style="font-family:Georgia;text-align:center; color:#CECECE;"> Re-engineering the Plas promoter's response to LasR. <br></p></div>
 +
<div class ="row-end"> </div>
 +
</div>
 +
 
 +
<div class="row">
 +
<div class="col12" id="spacer"></div>
 +
<div class="row-end"> </div>
 +
</div>
 +
 
 +
<br>
 +
<div class="row">
 +
 
 +
<div class="col4" align = "justify">
 +
<img src="https://static.igem.org/mediawiki/2011/b/bd/Aboutus.jpg">
 +
<p style="font-family:Georgia;color:black;font-size:14px;">
 +
We have all been brought together by a mutual interest for synthetic biology. It fascinates us how easily organisms can be tweaked and altered to our liking in this expansive field, so we thought it’d be best get started early. Through reading, lectures, mentorship and hands on experience we opened our minds and fueled our thirst for control over these malleable organisms. We functioned well together and played each other’s strengths for maximum efficiency while simultaneously having the time of our lives. We have learned much and hope to keep putting our best efforts forward till the very end.
 +
</p></div>
 +
 
 +
<div class = "col14">
 +
<p>
</p>
</p>
</div>
</div>
-
<div class="printfooter">
+
 
-
Retrieved from "<a href="https://2013hs.igem.org/wiki/index.php/Team:TPHS_SanDiego">https://2013hs.igem.org/wiki/index.php/Team:TPHS_SanDiego</a>"</div>
+
<div class="col8-4">
-
<div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content -->
+
 
-
<div class="visualClear"></div>
+
        <div class="slider-wrapper theme-default">
-
</div>
+
            <div class="ribbon"></div>
 +
            <div id="slider" class="nivoSlider">
 +
                <img src="https://static.igem.org/mediawiki/2013hs/a/ac/Geisel.jpg" width="450"/>
 +
                <img src="https://static.igem.org/mediawiki/2013hs/c/ce/Slideshow1.jpg" width="450"/>
 +
                <img src="https://static.igem.org/mediawiki/2013hs/3/35/Sunset-web.jpg" width="450"/>
 +
                <img src="https://static.igem.org/mediawiki/2013hs/a/af/Balpark.jpg" width="450"/>
 +
                <img src="https://static.igem.org/mediawiki/2013hs/0/07/Spenceandbran.jpg" width="450"/>
 +
                <img src="https://static.igem.org/mediawiki/2013hs/b/bd/Gaslamp-condos-lofts1.jpg" width="450"/>
 +
                <img src="https://static.igem.org/mediawiki/2013hs/0/07/Sunset2-web.jpg" width="450"/>
 +
 
 +
 
 +
            </div>
     </div>
     </div>
-
<!-- PAGE FOOTER -- ITEMS FROM COLUMN ! HAVE BEEN MOVED HERE  -- RDR  -->
 
-
<div class="visualClear"></div>
 
-
    <div id='footer-box' class='noprint'>
 
-
        <div id="footer">
 
-
              <div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div>       <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by/3.0/"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div>     <ul id="f-list">
 
-
 
-
  <!-- Recentchanges is not handles well DEBUG -->
+
    <script type="text/javascript">
-
    <li id="t-recentchanges"><a href="/wiki/index.php/Special:RecentChanges"
+
  $(window).load(function() {
-
      title='Recent changes'>Recent changes</a></li>
+
        $('#slider').nivoSlider();
 +
    });
 +
    </script>
 +
<br>
-
    <li id="t-whatlinkshere"><a href="/wiki/index.php/Special:WhatLinksHere/Team:TPHS_SanDiego"
+
<div id="fb-root"></div>
-
      title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li>
+
<script>(function(d, s, id) {
 +
  var js, fjs = d.getElementsByTagName(s)[0];
 +
  if (d.getElementById(id)) return;
 +
  js = d.createElement(s); js.id = id;
 +
  js.src = "//connect.facebook.net/en_US/all.js#xfbml=1";
 +
  fjs.parentNode.insertBefore(js, fjs);
 +
}(document, 'script', 'facebook-jssdk'));</script>
 +
<div class="fb-like" data-href="https://2013hs.igem.org/Team:TPHS_SanDiego" data-send="true" data-width="600" data-show-faces="false" data-font="arial"></div>
 +
</div>
 +
<div class="row-end"></div>
 +
</div>
 +
<div class = "row">
 +
<div class="col12-3">
 +
<p style="font-family:Georgia;color:black;font-size:16px;"> <b>Attributions:</b></p>
 +
<p style="font-family:Georgia;color:black;font-size:12px;"> All experiments explained here were done by the TPHS students, any previously existing plasmids and materials were supplied by Spencer Scott and the Hasty Lab at UCSD. The plate reader data acquisition was done at a lab where the high school students could not enter, but they are versed on every step of the process. The website layout was adapted from Berkeley iGEM 2011's wiki with their permission. Almost all edits were done by members of the high school team, including the home page, project page, protocols page, daily journal, safety page, and much of the team page. Some things such as the team photos and the parts page were done by Spencer Scott as an instructive lesson on design and using Illustrator and Photoshop. Anything done by an advisor was done as a teaching lesson with close interaction with the students at all times.
 +
</div>
 +
<div class="row-end"> </div>
 +
</div>
-
                <li id="t-specialpages"><a href="/wiki/index.php/Special:SpecialPages"
+
<div class="row">
-
                  title="List of all special pages [q]" accesskey="q">Special pages</a>
+
<div class="col12" id="spacer"></div>
-
                </li>
+
<div class="row-end"> </div>
-
                <li><a href='/Special:Preferences'>My preferences</a></li>
+
</div>
-
            </ul>
+
-
        </div> <!-- close footer -->
+
-
        <div id='footer'>
+
-
    <ul id="f-list">
+
-
            <li id="t-print"><a href="/wiki/index.php?title=Team:TPHS_SanDiego&amp;printable=yes"
+
<div class = "row">
-
              title="Printable version of this page [p]" accesskey="p">Printable version</a>
+
<div class="col12-3" style="background-color:#499394">
-
            </li>
+
<p style="text-align:center; color:#CECECE; font-size:13px; font-weight:bold; padding:3px"> The Torrey Pines High School iGEM team would like to thank New England Biolabs, the UCSD Biodynamics Labratory, and Mr. Brinn Belyea. </p>
 +
</div>
 +
<div class="row-end"> </div>
 +
</div>
-
        <li id="privacy"><a href="/wiki/index.php/2013hs.igem.org:Privacy_policy" title="2013hs.igem.org:Privacy policy">Privacy policy</a></li>
+
<div class="row">
-
        <li id="disclaimer"><a href="/wiki/index.php/2013hs.igem.org:General_disclaimer" title="2013hs.igem.org:General disclaimer">Disclaimers</a></li>
+
<div class="col12" id="spacer"></div>
-
    </ul>
+
<div class="row-end"> </div>
-
</div> <!-- close footer -->
+
-
    </div> <!-- close footer-box -->
+
-
+
-
<script>if (window.runOnloadHook) runOnloadHook();</script>
+
</div>
</div>
-
<!-- Served in 0.222 secs. --></body>
 
 +
<div class = "row">
 +
<div class="col12-3" >
 +
<img src="https://static.igem.org/mediawiki/2013hs/0/0d/LifeTechSponsor.png" width="300" >
 +
<img src="https://static.igem.org/mediawiki/2013hs/3/38/NEBSponsor.png" width="300" >
 +
<img src="https://static.igem.org/mediawiki/2013hs/d/d7/BDLLogo.png"width="300">
 +
<div class="row-end"></div>
 +
</div>
 +
 +
</div>
</html>
</html>
 +
 +
 +
{{Team:Berkeley/header}}
 +
{{Team:Berkeley/css}}
 +
{{Team:Berkeley/navigation}}

Latest revision as of 04:03, 22 June 2013

Berkeley iGEM 2011

header
Mercury

In an effort to expand the toolkit available to synthetic biologists, we've taken a system natively responsible for transcriptional activation and modified it to control transcriptional repression. The LasR system from Pseudomonas Aeruginosa requires the presence of a small molecule, C12-3-oxo-AHL, to induce activation of the Plas promoter. By modifying the -10 and -35 sites of the promoter, as well as shifting the location of the LasR binding sites, the new Plas promoter (Plas*) was changed from an inducible to a repressible promoter. Through adding this second functionality, the Plas* promoter could be used in conjunction with a wildtype Plas promoter to control two separate genes whose expression levels are always out of sync. Furthermore, if the bacteria are transfected with a plasmid encoding LasI, the bacteria will be able to turn off gene expression at a critical population density, instead of only being able to turn on gene expression.

Proteins discovered in Jellyfish that have the ability to emit fluorescence.

A clever way to normalize protein expression across cell populations.

A quorum sensing system from Pseudomonas aeruginosa.

Re-engineering the Plas promoter's response to LasR.


We have all been brought together by a mutual interest for synthetic biology. It fascinates us how easily organisms can be tweaked and altered to our liking in this expansive field, so we thought it’d be best get started early. Through reading, lectures, mentorship and hands on experience we opened our minds and fueled our thirst for control over these malleable organisms. We functioned well together and played each other’s strengths for maximum efficiency while simultaneously having the time of our lives. We have learned much and hope to keep putting our best efforts forward till the very end.


Attributions:

All experiments explained here were done by the TPHS students, any previously existing plasmids and materials were supplied by Spencer Scott and the Hasty Lab at UCSD. The plate reader data acquisition was done at a lab where the high school students could not enter, but they are versed on every step of the process. The website layout was adapted from Berkeley iGEM 2011's wiki with their permission. Almost all edits were done by members of the high school team, including the home page, project page, protocols page, daily journal, safety page, and much of the team page. Some things such as the team photos and the parts page were done by Spencer Scott as an instructive lesson on design and using Illustrator and Photoshop. Anything done by an advisor was done as a teaching lesson with close interaction with the students at all times.

The Torrey Pines High School iGEM team would like to thank New England Biolabs, the UCSD Biodynamics Labratory, and Mr. Brinn Belyea.


Retrieved from "http://2013hs.igem.org/Team:TPHS_SanDiego"