Team:St Pauls London/Notebook

2nd round of meetings - Admin and Research

1. Having settled on an allergen-detection mechanism, we pushed ahead with research regarding the “Ara” proteins, similar to food storage proteins found in many plants, and present on the surface of nuts (in particular peanuts).

Much research was done regarding the breakdown of Ara h 1 and Ara h 2, the two primary allergens present on the surface of peanuts. This research also encompassed the relative binding strengths of these two proteins with immunoassays (ELISAs and other serum-based testing methods).

In the end, the issue was raised that mediating the response via cAMP and a secondary messenger would be more difficult and require a receptor on the bacterial cell surface. This idea was therefore scrapped, as we could not find how the promoter/operons would be acted on by Ara h proteins.

2. Other allergens and molecules to which humans display adverse reactions were investigated. The most convenient of these was lactose.

Dr Shammas had mentioned the lac operon to us a few prior to the peanut allergen idea being scrapped. We therefore thought it convenient to look into this molecule, which we knew caused issues such as lactose intolerance.

3. We continued researching the lac operon, and starting looking at the kinds of things we could do. A vague idea of a traffic light system based on concentrations within the cell was devised.

4. At this point, we also finalised our application to the iGEM competition and paid the entrance fee.

1st round of meetings - Brainstorming

Ideas pitched:

1. “Operation Gloop” - production of a glue-like protein as found in mussels
2. Ionising Radiation detection
3. pH buffer - regulate acidity of soil for improved crop growth
4. Desalination
5. Organophosphate detection and degradation
6. Screening of blood (pathogen-sensing bacteria)
7. Chitinase-producing bacteria
8. Plastic/Artificial Polymer degradation
9. Allergen sensor

Issues raised with these ideas:

1. Protein may require post-translational modification; bacterium may not have necessary organelles
2. No simple detection mechanism
3. No simple mechanism
4. No simple mechanism; in addition, organism may die due to water potential differences
5. Major issue in that even in cleaving the phosphate group from the molecule, the long alkane chains in an of themselves may be toxic; in addition, a chemical breakdown mechanism which is both much faster and much more efficient than anything we could achieve already exists
6. Difficulty in finding specific proteins to trigger a promoter which are also present on bacteria
7. No simple mechanism/no biobrick
8. No simple mechanism; similar to organophosphate problem
9. Nuts may prove difficult, as allergenic proteins may not have a pathway into the cell to affect the promoter itself; secondary messenger system may have to be employed; could get quite messy