"
Team:The Agency Escondido/project
From 2013hs.igem.org
| Line 253: | Line 253: | ||
<!--<p>At the time of writing, the promoters to be used have not been selected, nor have the reporter genes been chosen.</p>--> | <!--<p>At the time of writing, the promoters to be used have not been selected, nor have the reporter genes been chosen.</p>--> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
<!--R0080 | <!--R0080 | ||
| Line 268: | Line 262: | ||
I12006 | I12006 | ||
C0051 inducer, Lambda cl repressor--> | C0051 inducer, Lambda cl repressor--> | ||
| - | <h2> Rock </h2 | + | <a id="rock"></a> |
| + | <h2> Rock </h2> | ||
| + | |||
| + | <p>The "rock" bacteria strain will have a CFP (<a href = "http://partsregistry.org/Part:BBa_E0020">part E0020</a>) and promoter (<a href = "http://partsregistry.org/Part:BBa_R0080">part R0080</a>). It will contain the LuxR gene (<a href = "http://partsregistry.org/Part:BBa_C0062">part C0062</a>) to induce the "paper" strain.</p> | ||
| + | <a id="paper"></a> | ||
<h2> Paper </h2> | <h2> Paper </h2> | ||
| + | |||
| + | <p>The "paper" bacteria strain will have an GFP (<a href = "http://partsregistry.org/Part:BBa_E0040">part E0040</a>) and promoter (<a href = "http://partsregistry.org/Part:BBa_I1051">part I1051</a>). It will contain the Lambda cl repressor gene (<a href = "http://partsregistry.org/Part:BBa_C0051">part C0051</a>) to induce the "scissors" strain.</p> | ||
| + | <a id="scissors"></a> | ||
<h2> Scissors </h2> | <h2> Scissors </h2> | ||
| + | |||
| + | <p>The "scissors" bacteria strain will have an RFP (<a href = "http://partsregistry.org/Part:BBa_E1010">part E1010</a>)and promoter (<a href = "http://partsregistry.org/Part:BBa_I12006">part I12006</a>). It will contain the AraC gene (<a href = "http://partsregistry.org/Part:BBa_C0080">part C0080</a>) to induce the "rock" strain.</p> | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
</html> | </html> | ||
Revision as of 20:24, 21 May 2013
Project Description
Our project seeks to create two identical triads of transparent bacteria strains with complementary hydrophobic inducer production to emulate the game of "rock, paper, scissors" (Hydrophobic proteins can move through plasma membranes). Each triad will correspond to a group: group A and group B. Each type of bacteria will produce a specific protein that will act as an inducer to one and only one other bacteria strain(rock strain, paper strain, and scissors strain). Each triad will have different reporter genes designating its champion color. When two bacteria strains are combined in an agar plate, each protein secreted by the victorious strain, when acting as an inducer, changes the color of the cells on the plate identifying the champion.
Rock
The "rock" bacteria strain will have a CFP (part E0020) and promoter (part R0080). It will contain the LuxR gene (part C0062) to induce the "paper" strain.
Paper
The "paper" bacteria strain will have an GFP (part E0040) and promoter (part I1051). It will contain the Lambda cl repressor gene (part C0051) to induce the "scissors" strain.
Scissors
The "scissors" bacteria strain will have an RFP (part E1010)and promoter (part I12006). It will contain the AraC gene (part C0080) to induce the "rock" strain.
