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Team:CIDEB-UANL Mexico/WetLab-Protocols
From 2013hs.igem.org
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<p> Laboratory Protocol </p> | <p> Laboratory Protocol </p> | ||
<p> Handling of lyophilized DNA’s plates. </p> | <p> Handling of lyophilized DNA’s plates. </p> | ||
| + | |||
| + | <p> This step is for searching and obtaining the desired piece. | ||
| + | <br>Procedure: | ||
| + | <br>1.- Search in the Parts Registry the BioBrick | ||
| + | <br>2.- Drill the plate, and add 10ul of mQ water | ||
| + | <br>3.- Mix very well, and take the liquid to an Eppendorf tube </p> | ||
| + | |||
| + | <p> Preparation for competent cells and their transformation. | ||
| + | <br>This is for preparing the plasmid in the bacterium with thermal shock | ||
| + | <br>Transformation Procedure: | ||
| + | <br>1.- Take 100ul of the bacteria to an Eppendorf tube | ||
| + | <br>2.- Add 2ul of DNA and mix them | ||
| + | <br>3.- Let in ice from 20 to 30 minutes | ||
| + | <br>4.- Dive the Eppendorfs with 42°C water for 1 minute | ||
| + | <br>5.- Take it to ice for 2 minutes | ||
| + | <br>6.- Add LB and incubate them for 20 min, then put them into petri dish at 37°C all the night. </p> | ||
Revision as of 02:08, 17 June 2013
LAB PROTOCOL
IGEM-CIDEB_UANL
General Rules
1. - Use always your lab coat
2. - Pick up always the essential material
3. - Never take any material to your mouth while you’re working with reactants
4. - Keep clean your working place
5. - Every scrap must be in the trash can
6. - Check gas and water keys, depending on your work
7. - Wash your hands before and after every session
8. - Always wear gloves for Ethidium bromide
9. - Save your material after working with it
10. - Every single question, accident, etc. tell to the instructor
Recommendations for Microbiology
11. - Sterilize with heat any crop material before and after using it.
12. - Don’t talk while you are working with sterilized material
13. - When start and finish your, sterilize the surface with 70% alcohol
14. - Al testing tubes, must be vertically
15. - Never deposit a living cultivation in the sump
16. - Sterilize every used material
Laboratory Protocol
Handling of lyophilized DNA’s plates.
This step is for searching and obtaining the desired piece.
Procedure:
1.- Search in the Parts Registry the BioBrick
2.- Drill the plate, and add 10ul of mQ water
3.- Mix very well, and take the liquid to an Eppendorf tube
Preparation for competent cells and their transformation.
This is for preparing the plasmid in the bacterium with thermal shock
Transformation Procedure:
1.- Take 100ul of the bacteria to an Eppendorf tube
2.- Add 2ul of DNA and mix them
3.- Let in ice from 20 to 30 minutes
4.- Dive the Eppendorfs with 42°C water for 1 minute
5.- Take it to ice for 2 minutes
6.- Add LB and incubate them for 20 min, then put them into petri dish at 37°C all the night.
