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Team:TPHS SanDiego/Project
From 2013hs.igem.org
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Our project focuses on promoter engineering. Our goal is to characterize a set of promoters (of our design) by moving the repressor and/or activator binding sites with respect to the -10 and -35 regions of the promoter. Ideally, we would like to show that by moving an activator binding site it can become a repressor and that by moving a repressor binding site it may become either irrelevant to transcription rate or even boost it. We also want to see if there is a steep decline in repressor/activator function as the binding site move along the promoter or if it is a gradual/linear change. We believe this project could have application to genetic circuits by allowing a single protein to either activate or repress a promoter depending on where the binding sites are placed on the promoter. | Our project focuses on promoter engineering. Our goal is to characterize a set of promoters (of our design) by moving the repressor and/or activator binding sites with respect to the -10 and -35 regions of the promoter. Ideally, we would like to show that by moving an activator binding site it can become a repressor and that by moving a repressor binding site it may become either irrelevant to transcription rate or even boost it. We also want to see if there is a steep decline in repressor/activator function as the binding site move along the promoter or if it is a gradual/linear change. We believe this project could have application to genetic circuits by allowing a single protein to either activate or repress a promoter depending on where the binding sites are placed on the promoter. | ||
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| - | <iframe src="https://docs.google.com/presentation/d/1yljSeVJrbB2XPS9ej2cHj8QVOD_JseTJ1Um48-RjfGs/embed?start=false&loop=false&delayms=3000" frameborder="0" width="760" height="549" allowfullscreen="true" mozallowfullscreen="true" webkitallowfullscreen="true"></iframe> | + | <iframe src="https://docs.google.com/presentation/d/1yljSeVJrbB2XPS9ej2cHj8QVOD_JseTJ1Um48-RjfGs/embed?start=false&loop=false&delayms=3000" frameborder="0" width="760" height="549" allowfullscreen="true" mozallowfullscreen="true" webkitallowfullscreen="true"></iframe></center> |
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Revision as of 18:06, 18 June 2013
Our project focuses on promoter engineering. Our goal is to characterize a set of promoters (of our design) by moving the repressor and/or activator binding sites with respect to the -10 and -35 regions of the promoter. Ideally, we would like to show that by moving an activator binding site it can become a repressor and that by moving a repressor binding site it may become either irrelevant to transcription rate or even boost it. We also want to see if there is a steep decline in repressor/activator function as the binding site move along the promoter or if it is a gradual/linear change. We believe this project could have application to genetic circuits by allowing a single protein to either activate or repress a promoter depending on where the binding sites are placed on the promoter.
