Team:St Pauls London/Parts

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HOME
HOME
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PROJECT
PROJECT
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----
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ABSTRACT
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PARTS
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----
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PROCEDURES
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----
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PROTOCOLS
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THE TEAM
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----
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PARTICIPANTS
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SUPERVISORS
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PROCEDURES
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OTHER
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HUMAN PRACTICES
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SAFETY
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ATTRIBUTIONS
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===Project===
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===Parts===
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:The school team is aiming to create a semi-quantitative detector for lactose, a sugar molecule which causes intolerance in 9% Caucasians, and between 70-90% in Asians and Africans. It is most commonly found in milk and dairy products and intolerance has been linked to recent over consumption of milk and dairy, particularly in Europe and North America. Individuals who are lactose intolerant have an insufficient lactase concentrations in their digestive tract. As a result they are unable to hydrolyse lactose into galactose and glucose. As a result these  disaccharide lactose molecules pass straight through the small intestine and into the colon. Bacteria in the colon can hydrolyse and then metabolise lactose and their anaerobic respiration of lactose produces C02 and CH4 in the colon. This leads to the bloated feeling and symptoms associated with intolerance. Unabsorbed glucose and galactose monosaccharides in the colon also reduce water potential in the colon leading to an influx of water and hence diarrhea.
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:We hope to go some way to fix this problem but providing the mechanism for a lactose detector. This should enable lactose intolerants to better decide what food produce to eat and help them avoid painful symptoms of intolerance. Furthermore the semi-quantitative nature of the project should enable people with a range of different tolerance levels to be catered for by the sensor; so individuals with higher lactose tolerance can eat more lactose rich food, but individuals with low tolerance can avoid it altogether.
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====Parts====
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====Circuits====
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=====Operon 1=====
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======Operon 1======
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======Operon 2======
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====Parts====
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In our notebook and on our procedures sheet, we refer to parts by letter. The main reason for applying arbitrary letter coded to all parts was to facilitate labelling of plates and tubes. We have included a table (below) with the full BioBrick equivalent of each letter-coded part, as well as its function. This may help other teams looking at our circuits and procedures better understand which parts perform which function.
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{|class="wikitable" style="text-align: center"
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!colspan="6" | PARTS
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|-
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|colspan="2" style="font-weight: bold"|Operon
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|style="font-weight: bold"|Letter Code
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|style="font-weight: bold"|BioBrick Reference
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|style="font-weight: bold"|Function
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|style="font-weight: bold"|Length (bp)
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|-
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|colspan="2" rowspan="3"|2
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|J
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K091104 BBa_K091104]
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|Promoter
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|87
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|-
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|K
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|[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]
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|RBS
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|12
 +
|-
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|L
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|[http://partsregistry.org/Part:BBa_E0030 BBa_E0030]
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|YFP gene
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|723
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|-
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|2
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|3
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|C
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|[http://partsregistry.org/Part:BBa_C0040 BBa_C0040]
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|TetR gene
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|685
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|-
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|2
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|3
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|F
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|[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
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|Terminator
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|129
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|-
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|colspan="2" rowspan="4"|3
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|A
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K418003 BBa_K418003]
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|Promoter
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|1416
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|-
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|B
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|[http://partsregistry.org/Part:BBa_B0033 BBa_B0033]
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|RBS
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|11
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|-
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|D
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|[http://partsregistry.org/Part:BBa_C0072 BBa_C0072]
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|mnt gene
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|313
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|-
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|E
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_E1010 BBa_E1010]
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|RFP gene
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|706
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|}
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</center>
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====Backbones====
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<center>
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{|class="wikitable" style="text-align: center"
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|-
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|style="font-weight: bold"|Backbone
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|style="font-weight: bold"|Length (bp)
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|-
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|Ampicillin-Resistant
 +
|2155
 +
|-
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|Kanamycin-Resistant
 +
|2204
 +
|-
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|Chloramphenicol-Resistant
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|2070
 +
|-
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|Tetracycline-Resistant
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|2461
 +
|-
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|Ampicillin- & Kanamycin-Resistant
 +
|3189
 +
|-
 +
|}
 +
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</center>
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Latest revision as of 19:28, 19 June 2013

Contents

Parts

Circuits

Operon 1

Operon 2

Operon 3

Parts

In our notebook and on our procedures sheet, we refer to parts by letter. The main reason for applying arbitrary letter coded to all parts was to facilitate labelling of plates and tubes. We have included a table (below) with the full BioBrick equivalent of each letter-coded part, as well as its function. This may help other teams looking at our circuits and procedures better understand which parts perform which function.

PARTS
Operon Letter Code BioBrick Reference Function Length (bp)
2 J [http://partsregistry.org/wiki/index.php?title=Part:BBa_K091104 BBa_K091104] Promoter 87
K [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] RBS 12
L [http://partsregistry.org/Part:BBa_E0030 BBa_E0030] YFP gene 723
2 3 C [http://partsregistry.org/Part:BBa_C0040 BBa_C0040] TetR gene 685
2 3 F [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] Terminator 129
3 A [http://partsregistry.org/wiki/index.php?title=Part:BBa_K418003 BBa_K418003] Promoter 1416
B [http://partsregistry.org/Part:BBa_B0033 BBa_B0033] RBS 11
D [http://partsregistry.org/Part:BBa_C0072 BBa_C0072] mnt gene 313
E [http://partsregistry.org/wiki/index.php?title=Part:BBa_E1010 BBa_E1010] RFP gene 706

Backbones

Backbone Length (bp)
Ampicillin-Resistant 2155
Kanamycin-Resistant 2204
Chloramphenicol-Resistant 2070
Tetracycline-Resistant 2461
Ampicillin- & Kanamycin-Resistant 3189

Retrieved from "http://2013hs.igem.org/Team:St_Pauls_London/Parts"