Team:Shenzhen SFLS/Project

From 2013hs.igem.org

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Revision as of 11:14, 30 March 2013

This is a template page. READ THESE INSTRUCTIONS.

You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.

You MUST have the following information on your wiki:

a team description
project description
safety information (did your team take a safety training course? were you supervised in the lab?)
team attribution (who did what part of your project?)

You may also wish to add other page such as:

lab notebook
sponsor information
other information

REMEMBER, keep all of your pages within your teams namespace.
Example: 2013hs.igem.org/Team:Deerfield_MA/Our_Pets

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)	Your team picture
	Team Shenzhen_SFLS

Official Team Profile

Student at SFLS want to use an enzyme known as “polyphosphate kinase”（PPK）to governance the eutrophication of water bodies. We know that Inorganic phosphate (Pi) is recognized as one of the major nutrients contributing to the outbreak of red tide.We have found that PPK is known to digest Inorganic phosphate (Pi). There is a small amount of it in e. coli and we want to copy the gene of PPK in the e.coli to make it more efficient,so the role of PPK will be more obvious Then we will use the GFP to express the result of the digest of PPK.We want to make two device in a gene circut to deal with this problem.

Device 1: We use a limitable promoter which would be limited by high concentrations of Pi, then the GFP in this device won't light and a protein we add in the device won't breed .

Device 2: If the protein didn't breed, the promoter in Device 2 will be induced and the PPK gene will work, it will digest Pi. When the concentration of phosphate become low, devive 2 will stop to digest Pi and the promoter in device 1 will be induced, then the GFP in device 1 will light. That means the work has finished.

( We will put on our picture of gene circuts in few days)

Notebook

Show us how you spent your days.

Results/Conclusions

What did you achieve over the course of your semester?

Safety

What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?

Attributions

Who worked on what?

Human Practices

What impact does/will your project have on the public?

Fun!

What was your favorite team snack?? Have a picture of your team mascot?

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 ===Project===
-Student at SFLS want to produce an enzyme known as “polyphosphate kinase”（PPK）to governance the eutrophication of water bodies. We know that the eutrophication of water bodies is mainly caused by Pi in water. If the the concentration of phosphate in the water is too high, ther will have a lot of trouble such as red tide in lake, sea and river.PPK is known to digest Pi (phosphate). We want to make two device in a gene circut to deal with this problem.
+Student at SFLS want to use an enzyme known as “polyphosphate kinase”（PPK）to governance the eutrophication of water bodies. We know that Inorganic phosphate (Pi) is recognized as one of the major nutrients contributing to the outbreak of red tide.We have found that PPK is known to digest Inorganic phosphate (Pi). There is a small amount of it in e. coli and we want to copy the gene of PPK in the e.coli to make it more efficient,so the role of PPK will be more obvious Then we will use the GFP to express the result of the digest of PPK.We want to make two device in a gene circut to deal with this problem.
-Device 1: We use a limitable promoter which would be limited by high concentrations of Pi, then the GFP in this device won't light and a protein we add in the device won't breed too.
+Device 1: We use a limitable promoter which would be limited by high concentrations of Pi, then the GFP in this device won't light and a protein we add in the device won't breed .