Team:Jefferson VA SciCOS

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'''''Project'''
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This is a template page. READ THESE INSTRUCTIONS.
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The amount of oxygenation at ulcer sites is a key determinant of the outcome of healing because oxygen is crucial to the healing process and for resistance to infection. The non-healing skin ulcers that are formed from diabetic gangrene tend to be hypoxic; hypoxia is a condition not favorable to tissue repair. According to numerous studies, topical treatments of growth factors and peripheral blood mononuclear cells can help treat ulcers non-invasively in gangrene patients. It is posited that the growth factors promote angiogenesis, the physiological process by which new blood vessels form from pre-existing vessels. It is also a vital process in growth and development as well as in repair of damaged tissue. Furthermore, angiogenesis can render greater oxygen delivery to wounded areas, further enhancing growth and recovery to the areas suffering from chronic ischemia. In order to promote angiogenesis in gangrenous sites, and in turn treat the ulcers, growth factors such as fibroblast growth factor (FGF), vascular endothelial growth factor (VGF), and keratinocyte growth factor (KGF) can be produced in elevated quantities. To address the issue of inadequate oxygen levels during the healing process, we coupled the VHb gene promoter, which encodes for the Vitreoscilla hemoglobin molecule, to a downstream gene encoding KGF. The VHb promoter is most activated at an oxygen threshold of 2%, allowing for maximum transcription of the KGF gene and production of the growth factor in response to conditions of hypoxia. We also wanted to tested the production of another essential growth factor, FGF, under the influence of various constitutive promoters. This oxygen-sensing device is part of an array of growing applications targeted towards the promotion of angiogenesis as a means of healing in patients with severe, diabetic gangrene.
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have the following information on your wiki:
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#slideshow {
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<li>a team description</li>  
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REMEMBER, keep all of your pages within your teams namespace. <br><span style="font-weight:normal; font-style:italic;">Example: 2013hs.igem.org/Team:Jefferson_VA_SciCOS/Our_Pets</span>
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|[[File:Image1.jpg|261px]][[File:Transparent.png|65px]][[File:Transparent.png|100px]][[File:Transparent.png|100px]][[File:Transparent.png|100px]][[File:Transparent.png|65px]][[File:Image2.jpg|261px]]
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
 
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|[[Image:Jefferson_VA_SciCOS_logo.png|200px|right|frame]]
 
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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|[[Image:Jefferson_VA_SciCOS_team.png|right|frame|Your team picture]]
 
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|align="center"|[[Team:Jefferson_VA_SciCOS | Team Jefferson_VA_SciCOS]]
 
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=Jefferson_VA_SciCOS Official Team Profile]
 
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===Team===
 
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Tell us about your team, your school!
 
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===Project===
 
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What are you working on this semester?
 
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===Notebook===
 
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Show us how you spent your days.
 
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===Results/Conclusions===
 
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What did you achieve over the course of your semester?
 
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===Safety===
 
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What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
 
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===Attributions===
 
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Who worked on what?
 
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===Human Practices===
 
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===Fun!===
 
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'''''Background'''
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Established in 1985, Thomas Jefferson High School for Science and Technology is the result of a partnership of businesses and schools created to improve education in science, mathematics, and technology. Representatives from business and industry and staff of the Fairfax County Public Schools worked together in curriculum and facilities development for the school. In recent years, local business leaders and Jefferson parents have formed the Jefferson Partnership Fund to help raise money to maintain and equip labs and classrooms in the school. As the Governor's School for Science and Technology in Northern Virginia, the school is also supported by the Virginia Department of Education. In addition to providing a specialized education for selected students in Fairfax County, Jefferson also serves other school districts including Arlington, Loudoun, and Prince William counties as well as the cities of Fairfax and Falls Church.
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[[Image:Jefferson_VA_SciCOS_logo_colored.png|180px|right]]
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'''''Apotex Incorporated'''
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We would like to thank Apotex Inc. for their generous sponsorship of our project. Without their donations and support, much of the work that we did would not be possible.
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[[Image:Apotex.png|180px|right]]
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'''''Mentors'''
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We would also like to thank Dr. Mary Susan Burnett, a DNA teacher, researcher, and lab director who is currently employed at the Thomas Jefferson High School for Science and Technology. She guided us through the more difficult procedures in our experiment, and taught us all of the proper techniques and safety guidelines that we employed throughout our work.
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[[Image:Burnett.png|90px|right]]
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''Website designed and created by [https://2013hs.igem.org/Team:Jefferson_VA_SciCOS/Team Lucas Kang].''
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Latest revision as of 03:06, 22 June 2013

Project

The amount of oxygenation at ulcer sites is a key determinant of the outcome of healing because oxygen is crucial to the healing process and for resistance to infection. The non-healing skin ulcers that are formed from diabetic gangrene tend to be hypoxic; hypoxia is a condition not favorable to tissue repair. According to numerous studies, topical treatments of growth factors and peripheral blood mononuclear cells can help treat ulcers non-invasively in gangrene patients. It is posited that the growth factors promote angiogenesis, the physiological process by which new blood vessels form from pre-existing vessels. It is also a vital process in growth and development as well as in repair of damaged tissue. Furthermore, angiogenesis can render greater oxygen delivery to wounded areas, further enhancing growth and recovery to the areas suffering from chronic ischemia. In order to promote angiogenesis in gangrenous sites, and in turn treat the ulcers, growth factors such as fibroblast growth factor (FGF), vascular endothelial growth factor (VGF), and keratinocyte growth factor (KGF) can be produced in elevated quantities. To address the issue of inadequate oxygen levels during the healing process, we coupled the VHb gene promoter, which encodes for the Vitreoscilla hemoglobin molecule, to a downstream gene encoding KGF. The VHb promoter is most activated at an oxygen threshold of 2%, allowing for maximum transcription of the KGF gene and production of the growth factor in response to conditions of hypoxia. We also wanted to tested the production of another essential growth factor, FGF, under the influence of various constitutive promoters. This oxygen-sensing device is part of an array of growing applications targeted towards the promotion of angiogenesis as a means of healing in patients with severe, diabetic gangrene.

Image1.jpgTransparent.pngTransparent.pngTransparent.pngTransparent.pngTransparent.png261px












Background

Established in 1985, Thomas Jefferson High School for Science and Technology is the result of a partnership of businesses and schools created to improve education in science, mathematics, and technology. Representatives from business and industry and staff of the Fairfax County Public Schools worked together in curriculum and facilities development for the school. In recent years, local business leaders and Jefferson parents have formed the Jefferson Partnership Fund to help raise money to maintain and equip labs and classrooms in the school. As the Governor's School for Science and Technology in Northern Virginia, the school is also supported by the Virginia Department of Education. In addition to providing a specialized education for selected students in Fairfax County, Jefferson also serves other school districts including Arlington, Loudoun, and Prince William counties as well as the cities of Fairfax and Falls Church.


Jefferson VA SciCOS logo colored.png

Apotex Incorporated

We would like to thank Apotex Inc. for their generous sponsorship of our project. Without their donations and support, much of the work that we did would not be possible.

Apotex.png

Mentors

We would also like to thank Dr. Mary Susan Burnett, a DNA teacher, researcher, and lab director who is currently employed at the Thomas Jefferson High School for Science and Technology. She guided us through the more difficult procedures in our experiment, and taught us all of the proper techniques and safety guidelines that we employed throughout our work.

Burnett.png


Website designed and created by Lucas Kang.

Retrieved from "http://2013hs.igem.org/Team:Jefferson_VA_SciCOS"