Team:St Pauls London/Procedures

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HOME
HOME
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PROJECT
PROJECT
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ABSTRACT
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PARTS
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PROCEDURES
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PROTOCOLS
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THE TEAM
THE TEAM
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PARTICIPANTS
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SUPERVISORS
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PROCEDURES
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OTHER
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NOTEBOOK
NOTEBOOK
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HUMAN PRACTICES
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SAFETY
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===Procedures===
===Procedures===
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:Our project involves various different digest, ligation, transformation, purification and extraction steps. For each step we used a standardised procedure as detailed below.
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:Our project involves various different digest, ligation, transformation, purification and extraction steps. The PDFs below illustrate which methods we used (and at which stage they were used) during the construction of our operons.
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=====Operon 1=====
=====Operon 1=====
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Fortunately, this entire operon came as one single BioBrick, allowing us to save a lot of time by simply using the agar stab sent to us by iGEM HQ (after we had incorrectly resuspended the lyophilised plasmid) as our operon-containing bacterium.
=====Operon 2=====
=====Operon 2=====
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====Restriction Digest====
 
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====Ligation====
 
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====Transformation Purification and Extraction====
 
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====Safety====
 

Latest revision as of 11:47, 7 June 2013

Contents

Procedures

Our project involves various different digest, ligation, transformation, purification and extraction steps. The PDFs below illustrate which methods we used (and at which stage they were used) during the construction of our operons.

Operons

Operon 1

Fortunately, this entire operon came as one single BioBrick, allowing us to save a lot of time by simply using the agar stab sent to us by iGEM HQ (after we had incorrectly resuspended the lyophilised plasmid) as our operon-containing bacterium.

Operon 2

Operon 3


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