Team:St Pauls London/Parts

From 2013hs.igem.org

(Difference between revisions)
(→Operon 3)
 
(22 intermediate revisions not shown)
Line 39: Line 39:
                 display: none;
                 display: none;
         }
         }
-
+
 
/*----- HEADER -----*/
/*----- HEADER -----*/
#header {
#header {
Line 45: Line 45:
height: 150px;
height: 150px;
}
}
-
 
+
-
       
+
#headerTitle {
#headerTitle {
text-align: right;
text-align: right;
Line 99: Line 98:
color: #F93;
color: #F93;
text-decoration: underline;
text-decoration: underline;
-
}
+
}    
-
 
+
-
         a.no-decoration {
+
         a.mobile-link, a.mobile-link:hover, a.mobile-link:active {
-
                 text-decoration: none;
+
                 text-decoration:none;
-
                color: inherit;
+
         }
         }
Line 128: Line 126:
         div.drop-down-top {
         div.drop-down-top {
                 background-color: transparent;
                 background-color: transparent;
-
                 margin-bottom: -6px;
+
                 margin-bottom: -7px;
         }
         }
Line 135: Line 133:
         }
         }
-
         ul.drop-down li.top-of-list {
+
         ul.drop-down li.top-of-list { /*----- makes sure top item is always shown -----*/
                 display: block;
                 display: block;
-
                background-color: transparent;
 
-
                color: black;
 
-
                text-decoration: none;
 
         }
         }
                
                
Line 148: Line 143:
         ul.drop-down:hover li.top-of-list {
         ul.drop-down:hover li.top-of-list {
                 color: #F93;
                 color: #F93;
-
text-decoration: underline;
+
text-decoration: underline;            
         }
         }
Line 154: Line 149:
/*----- MAIN CONTENT -----*/
/*----- MAIN CONTENT -----*/
-
 
-
        .indent-content {
 
-
                margin-left: 25px;
 
-
        }
 
-
 
-
        .pdf {
 
-
                text-align: center;
 
-
        }
 
.title-link, .title-link:active, .title-link:visited {
.title-link, .title-link:active, .title-link:visited {
Line 227: Line 214:
   <ul class="drop-down">
   <ul class="drop-down">
   <div class="drop-down-top">
   <div class="drop-down-top">
-
   <li class="top-of-list">
+
   <li class="top-of-list"> 
 +
    <a class="mobile-link" href="">
     <div class="nav_button">
     <div class="nav_button">
     <p class="nav_buttonText">
     <p class="nav_buttonText">
Line 233: Line 221:
PROJECT
PROJECT
<html>
<html>
-
   </p></div></li></div>
+
   </p></div></a></li></div>
</html>
</html>
<html><div class="drop-down"><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Abstract"></html>
<html><div class="drop-down"><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Abstract"></html>
Line 258: Line 246:
   <div class="drop-down-top">
   <div class="drop-down-top">
   <li class="top-of-list">
   <li class="top-of-list">
 +
    <a class="mobile-link" href="">
     <div class="nav_button">
     <div class="nav_button">
     <p class="nav_buttonText">
     <p class="nav_buttonText">
Line 263: Line 252:
THE TEAM
THE TEAM
<html>
<html>
-
   </p></div></li></div>
+
   </p></div></a></li></div>
</html>
</html>
<html><div class="drop-down"><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Participants"></html>
<html><div class="drop-down"><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Participants"></html>
Line 280: Line 269:
   <div class="drop-down-top">
   <div class="drop-down-top">
   <li class="top-of-list">
   <li class="top-of-list">
 +
    <a style="text-decoration: none" href="">
     <div class="nav_button">
     <div class="nav_button">
     <p class="nav_buttonText">
     <p class="nav_buttonText">
Line 285: Line 275:
OTHER
OTHER
<html>
<html>
-
   </p></div></li></div>
+
   </p></div></a></li></div>
</html>
</html>
<html><div class="drop-down"><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Notebook"></html>
<html><div class="drop-down"><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Notebook"></html>
Line 298: Line 288:
<html><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Safety"></html>
<html><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Safety"></html>
SAFETY
SAFETY
 +
----
 +
<html></a></li></html>
 +
<html><li><a class="nav_buttonLink" href="https://2013hs.igem.org/Team:St_Pauls_London/Attributions"></html>
 +
ATTRIBUTIONS
----
----
<html></a></li></html>
<html></a></li></html>
Line 334: Line 328:
<center>
<center>
<html>
<html>
-
   <img src="https://static.igem.org/mediawiki/2013hs/4/4d/Operon_3.png"/>
+
   <img src="https://static.igem.org/mediawiki/2013hs/2/2e/Operon_3_new.png"/>
</html>
</html>
</center>
</center>
-
 
====Parts====
====Parts====
Line 346: Line 339:
{|class="wikitable" style="text-align: center"
{|class="wikitable" style="text-align: center"
-
  !colspan="4" | PARTS
+
  !colspan="6" | PARTS
  |-
  |-
-
  |colspan="2"|Operon
+
  |colspan="2" style="font-weight: bold"|Operon
-
  |Letter Code
+
  |style="font-weight: bold"|Letter Code
-
  |BioBrick Reference
+
  |style="font-weight: bold"|BioBrick Reference
-
  |Function
+
  |style="font-weight: bold"|Function
 +
|style="font-weight: bold"|Length (bp)
  |-
  |-
-
  |colspan="2"|2
+
  |colspan="2" rowspan="3"|2
  |J
  |J
-
  |BBa_K091104
+
  |[http://partsregistry.org/wiki/index.php?title=Part:BBa_K091104 BBa_K091104]
  |Promoter
  |Promoter
 +
|87
  |-
  |-
-
|colspan="2"|2
 
  |K
  |K
-
  |BBa_B0034
+
  |[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]
  |RBS
  |RBS
 +
|12
  |-
  |-
-
|colspan="2"|2
 
  |L
  |L
-
  |BBa_E0030
+
  |[http://partsregistry.org/Part:BBa_E0030 BBa_E0030]
  |YFP gene
  |YFP gene
 +
|723
  |-
  |-
  |2
  |2
  |3
  |3
  |C
  |C
-
  |BBa_C0040
+
  |[http://partsregistry.org/Part:BBa_C0040 BBa_C0040]
  |TetR gene
  |TetR gene
 +
|685
  |-
  |-
  |2
  |2
  |3
  |3
  |F
  |F
-
  |BBa_B0015
+
  |[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
  |Terminator
  |Terminator
 +
|129
  |-
  |-
-
  |colspan="2"|3
+
  |colspan="2" rowspan="4"|3
  |A
  |A
-
  |BBa_K418003
+
  |[http://partsregistry.org/wiki/index.php?title=Part:BBa_K418003 BBa_K418003]
  |Promoter
  |Promoter
 +
|1416
  |-
  |-
-
|colspan="2"|3
 
  |B
  |B
-
  |BBa_B0033
+
  |[http://partsregistry.org/Part:BBa_B0033 BBa_B0033]
  |RBS
  |RBS
 +
|11
  |-
  |-
-
|colspan="2"|3
 
  |D
  |D
-
  |BBa_C0072
+
  |[http://partsregistry.org/Part:BBa_C0072 BBa_C0072]
-
  |Lacl gene
+
  |mnt gene
 +
|313
  |-
  |-
-
|colspan="2"|3
 
  |E
  |E
-
  |BBa_E1010
+
  |[http://partsregistry.org/wiki/index.php?title=Part:BBa_E1010 BBa_E1010]
  |RFP gene
  |RFP gene
 +
|706
 +
|}
 +
</center>
 +
 +
====Backbones====
 +
 +
<center>
 +
 +
{|class="wikitable" style="text-align: center"
 +
 +
|-
 +
|style="font-weight: bold"|Backbone
 +
|style="font-weight: bold"|Length (bp)
 +
|-
 +
|Ampicillin-Resistant
 +
|2155
 +
|-
 +
|Kanamycin-Resistant
 +
|2204
 +
|-
 +
|Chloramphenicol-Resistant
 +
|2070
 +
|-
 +
|Tetracycline-Resistant
 +
|2461
 +
|-
 +
|Ampicillin- & Kanamycin-Resistant
 +
|3189
 +
|-
 +
|}
 +
</center>
</center>
<html></div></html>
<html></div></html>

Latest revision as of 19:28, 19 June 2013

Contents

Parts

Circuits

Operon 1

Operon 2

Operon 3

Parts

In our notebook and on our procedures sheet, we refer to parts by letter. The main reason for applying arbitrary letter coded to all parts was to facilitate labelling of plates and tubes. We have included a table (below) with the full BioBrick equivalent of each letter-coded part, as well as its function. This may help other teams looking at our circuits and procedures better understand which parts perform which function.

PARTS
Operon Letter Code BioBrick Reference Function Length (bp)
2 J [http://partsregistry.org/wiki/index.php?title=Part:BBa_K091104 BBa_K091104] Promoter 87
K [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] RBS 12
L [http://partsregistry.org/Part:BBa_E0030 BBa_E0030] YFP gene 723
2 3 C [http://partsregistry.org/Part:BBa_C0040 BBa_C0040] TetR gene 685
2 3 F [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] Terminator 129
3 A [http://partsregistry.org/wiki/index.php?title=Part:BBa_K418003 BBa_K418003] Promoter 1416
B [http://partsregistry.org/Part:BBa_B0033 BBa_B0033] RBS 11
D [http://partsregistry.org/Part:BBa_C0072 BBa_C0072] mnt gene 313
E [http://partsregistry.org/wiki/index.php?title=Part:BBa_E1010 BBa_E1010] RFP gene 706

Backbones

Backbone Length (bp)
Ampicillin-Resistant 2155
Kanamycin-Resistant 2204
Chloramphenicol-Resistant 2070
Tetracycline-Resistant 2461
Ampicillin- & Kanamycin-Resistant 3189

Retrieved from "http://2013hs.igem.org/Team:St_Pauls_London/Parts"