Team:The Agency Escondido/project/scissors

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                 <a href="http://agency-stem.org"><img width=500px src="http://agency-stem.org/static/img/2013-heading-web.png" alt="Agency Logo"/></a>
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                 <a href="http://agency-stem.org"><img width=500px src="https://static.igem.org/mediawiki/igem.org/b/bb/Agency-2013-heading-web.png" alt="Agency Logo"/></a>
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<li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido">About</a></li>
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    <li>
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<li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido/notebook">Notebook</a></li>
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        <a href="https://2013hs.igem.org/Team:The_Agency_Escondido">About</a>
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<li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido/safety">Safety</a></li>
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<li id="selectedsection">Project</a></li>
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            <li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido">General</a></li>
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<li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido/sandiego">San Diego</a></li>
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            <li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido/sandiego">San Diego</a></li>
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<li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido/ourteam">Our Team</a></li>
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            <li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido/ourteam">Our Team</a></li>
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        </ul>
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        <a href="https://2013hs.igem.org/Team:The_Agency_Escondido/project"><u>Project</u></a>
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            <li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido/project/rock">Rock</a></li>
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            <li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido/project/paper">Paper</a></li>
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            <li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido/project/scissors">Scissors</a></li>
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        </ul>
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    </li>
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    <li>
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        <a href="https://2013hs.igem.org/Team:The_Agency_Escondido/notebook">Notebook</a>
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        <ul>
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            <li></li>
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        </ul>
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    </li>
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    <li>
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        <a href="https://2013hs.igem.org/Team:The_Agency_Escondido/safety">Safety</a>
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        <ul>
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            <li></li>
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Project Description
 
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Our project seeks to create two identical triads of transparent bacteria strains with complementary hydrophobic inducer production to emulate the game of "rock, paper, scissors" (Hydrophobic proteins can move through plasma membranes). Each triad will correspond to a group: group A and group B. Each type of bacteria will produce a specific protein that will act as an inducer to one and only one other bacteria strain(rock strain, paper strain, and scissors strain). Each triad will have different reporter genes designating its champion color. When two bacteria strains are combined in an agar plate, each protein secreted by the victorious strain, when acting as an inducer, changes the color of the cells on the plate identifying the champion.
 
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<h2> Scissors </h2>  
<h2> Scissors </h2>  
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<h4>May 31</h4><p>Vansh Singh's plates showed no growth.</p>
<h4>May 31</h4><p>Vansh Singh's plates showed no growth.</p>
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<h4>June 3</h4><p>Vansh Singh doubted his ligation and <a href = "http://casp.agency-stem.org/media/G13-LP0008-27.pdf">ligated</a> another batch of Part X plasmids.</p>
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<h4>June 4</h4><p>Vansh Singh and Evan Santos started the protocol for calcium chloride competent cells. The cells were incubated on ice over the night.</p>
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<h4>June 5</h4><p>Vansh Singh and Evan Santos finished the calcium chloride competent cell protocol and transformed Part X with the competent cells. Because of the lack of LB agar plates, he left a 1.5ml eppendorf tube with the transformed cells and LB + chloremphenicol in the dry bath set at 37 degrees Celsius.</p>
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<h4>June 6</h4><p>Vansh Singh inoculated a 5ml tube of LB media + chloremphenicol from his eppendorf tube. We still lacked chloremphenicol agar plates.</p>
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<h4>June 7</h4><p>Yesterday's chloremphenicol tube showed significant cloudiness. He believes that he successfully assembled Part X. Mentor Andrew Segina made 4 LB Agar + kanamayacin plates. Unfortunately Vansh needed chloremphenicol plates. So, Vansh made inoculated a new tube of LB + chloremphenicol from the tube from yesterday.</p>
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<h4>June 10</h4><p>Vansh Singh made 4 <a href = "http://casp.agency-stem.org/media/G13-LP0002-74.pdf">LB Agar</a> + chloremphenicol plates. He inoculated a plate from his "Part X cells 6/6/13" tube.</p>
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<h4>June 11</h4><p>Vansh Singh believed that he saw a colony on his Part X plate. So, he inoculated a flask (We were out of clean tubes) of 10mL LB + Chloremphenicol with the colony on the Part X plate. A single clonal line of <a href = "http://casp.agency-stem.org/media/G13-LP0010-57.pdf">Assembly one</a> has successfully been grown.</p>
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<h4>June 12</h4><p>Vansh Singh miniprepped Part X according to the <a href = "https://www.promega.com/~/media/Files/Resources/ProtCards/PureYield%20Plasmid%20Miniprep%20System%20Quick%20Protocol.pdf">Promega PureYield(TM) Plasmid Miniprep System</a>.</p>
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<h4>June 13</h4><p>Vansh Singh completed the digestion and ligation of <a href = "http://casp.agency-stem.org/media/G13-LP0010-57.pdf">Assemly 2</a></p>
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Latest revision as of 19:36, 18 June 2013