Team:The Agency Escondido

From 2013hs.igem.org

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             needed to each occasion. Our efforts are focused in areas of science, technology, engineering, and mathematics
             needed to each occasion. Our efforts are focused in areas of science, technology, engineering, and mathematics
             (STEM).</p>
             (STEM).</p>
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Project Description
 
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Our project seeks to create two identical triads of transparent bacteria strains with complementary hydrophobic inducer production to emulate the game of "rock, paper, scissors" (Hydrophobic proteins can move through plasma membranes). Each triad will correspond to a group: group A and group B. Each type of bacteria will produce a specific protein that will act as an inducer to one and only one other bacteria strain(rock strain, paper strain, and scissors strain). Each triad will have different reporter genes designating its champion color. When two bacteria strains are combined in an agar plate, each protein secreted by the victorious strain, when acting as an inducer, changes the color of the cells on the plate identifying the champion.
 
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<!--When two of the three types of bacteria are combined and exposed to an environmental stimulus, one type will resist the antibiotic produced by the other, while the other will be vulnerable to the antibiotic produced by the first. The first type will therefore spread freely through the media while the growth of the second will be inhibited. To identify the "victorious" strain, each will include a different fluorescent reporter gene.--> </p>
 
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<!--<p>At the time of writing, the promoters to be used have not been selected, nor have the reporter genes been chosen.</p>-->
 
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<p>The "rock" bacteria strain will have a CFP (<a href = "http://partsregistry.org/Part:BBa_E0020">part E0020</a>) and promoter (<a href = "http://partsregistry.org/Part:BBa_R0080">part R0080</a>). It will contain the LuxR gene (<a href = "http://partsregistry.org/Part:BBa_C0062">part C0062</a>) to induce the "paper" strain.</p>
 
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<p>The "scissors" bacteria strain will have an RFP (<a href = "http://partsregistry.org/Part:BBa_E1010">part E1010</a>)and promoter (<a href = "http://partsregistry.org/Part:BBa_I12006">part I12006</a>). It will contain the AraC gene (<a href = "http://partsregistry.org/Part:BBa_C0080">part C0080</a>) to induce the "rock" strain.</p>
 
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<p>The "paper" bacteria strain will have an GFP (<a href = "http://partsregistry.org/Part:BBa_E0040">part E0040</a>) and promoter (<a href = "http://partsregistry.org/Part:BBa_I1051">part I1051</a>). It will contain the Lambda cl repressor gene (<a href = "http://partsregistry.org/Part:BBa_C0051">part C0051</a>) to induce the "scissors" strain.</p>
 
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C0080 inducer, Arac
 
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C0062 inducer, LuxR
 
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I12006
 
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C0051 inducer, Lambda cl repressor-->
 
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Revision as of 20:02, 21 May 2013

Sponsored by

  • Genentech Logo
  • MathWorks Logo

Team Description

The Agency is an organization of students, parents, faculty and mentors operating out of the Classical Academy High School of Escondido, CA. Our objective is to compete in challenging venues, and to foster the development of skills and character needed to each occasion. Our efforts are focused in areas of science, technology, engineering, and mathematics (STEM).

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