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Team:TPHS SanDiego/Protocol
From 2013hs.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
Put protocol here! | Put protocol here! | ||
| - | CPEC | + | CPEC, |
| - | Wobble | + | Wobble, |
| - | EIPCR (enzymatic inverse PCR) | + | EIPCR (enzymatic inverse PCR) , |
| - | Normal ligation assembly (3A assembly) | + | Normal ligation assembly (3A assembly), |
| - | Mini-preps | + | Mini-preps, |
| - | Transformations | + | Transformations, |
| - | Ligations | + | Ligations, |
| - | Digestions | + | Digestions, |
| - | Normal PCRs | + | Normal PCRs, |
| - | Gel-extractions | + | Gel-extractions, |
| - | PCR-purifications | + | PCR-purifications, |
| - | Running electrophoresis gels | + | Running electrophoresis gels, |
| - | Comp cells | + | Comp cells, |
| - | Plating | + | Plating, |
| - | Overnight cultures (liquid cultures) | + | Overnight cultures (liquid cultures), |
Denote when the UCSD services had to be used – sequencing, designing primers, data managing techniques, ordering primers, thermocycler | Denote when the UCSD services had to be used – sequencing, designing primers, data managing techniques, ordering primers, thermocycler | ||
</html> | </html> | ||
Revision as of 04:58, 14 May 2013
Put protocol here! CPEC, Wobble, EIPCR (enzymatic inverse PCR) , Normal ligation assembly (3A assembly), Mini-preps, Transformations, Ligations, Digestions, Normal PCRs, Gel-extractions, PCR-purifications, Running electrophoresis gels, Comp cells, Plating, Overnight cultures (liquid cultures), Denote when the UCSD services had to be used – sequencing, designing primers, data managing techniques, ordering primers, thermocycler
