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Team:TPHS SanDiego/Protocol
From 2013hs.igem.org
(Difference between revisions)
| Line 5: | Line 5: | ||
CPEC, | CPEC, | ||
Wobble, | Wobble, | ||
| - | EIPCR (enzymatic inverse PCR) | + | EIPCR (enzymatic inverse PCR) <br> |
| - | Normal ligation assembly (3A assembly) | + | Normal ligation assembly (3A assembly) <br> |
| - | Mini-preps | + | Mini-preps <br> |
| - | Transformations | + | Transformations <br> |
| - | Ligations | + | Ligations <br> |
| - | Digestions | + | Digestions <br> |
| - | Normal PCRs | + | Normal PCRs <br> |
| - | Gel-extractions | + | Gel-extractions <br> |
| - | PCR-purifications | + | PCR-purifications <br> |
| - | Running electrophoresis gels | + | Running electrophoresis gels <br> |
| - | Comp cells | + | Comp cells <br> |
| - | Plating | + | Plating <br> |
| - | Overnight cultures (liquid cultures) | + | Overnight cultures (liquid cultures) <br> |
Denote when the UCSD services had to be used – sequencing, designing primers, data managing techniques, ordering primers, thermocycler | Denote when the UCSD services had to be used – sequencing, designing primers, data managing techniques, ordering primers, thermocycler | ||
</html> | </html> | ||
Revision as of 05:38, 14 May 2013
Put protocol here!
CPEC,
Wobble,
EIPCR (enzymatic inverse PCR)
Normal ligation assembly (3A assembly)
Mini-preps
Transformations
Ligations
Digestions
Normal PCRs
Gel-extractions
PCR-purifications
Running electrophoresis gels
Comp cells
Plating
Overnight cultures (liquid cultures)
Denote when the UCSD services had to be used – sequencing, designing primers, data managing techniques, ordering primers, thermocycler
