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Team:TPHS SanDiego/Protocol
From 2013hs.igem.org
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| - | Put protocol here! | + | Put protocol here! <br> |
| - | + | <br> | |
| - | CPEC | + | <br> |
| - | Wobble | + | CPEC <br> |
| + | Wobble <br> | ||
EIPCR (enzymatic inverse PCR) <br> | EIPCR (enzymatic inverse PCR) <br> | ||
| + | |||
Normal ligation assembly (3A assembly) <br> | Normal ligation assembly (3A assembly) <br> | ||
Mini-preps <br> | Mini-preps <br> | ||
Revision as of 05:39, 14 May 2013
Put protocol here!
CPEC
Wobble
EIPCR (enzymatic inverse PCR)
Normal ligation assembly (3A assembly)
Mini-preps
Transformations
Ligations
Digestions
Normal PCRs
Gel-extractions
PCR-purifications
Running electrophoresis gels
Comp cells
Plating
Overnight cultures (liquid cultures)
Denote when the UCSD services had to be used – sequencing, designing primers, data managing techniques, ordering primers, thermocycler
