Team:Lethbridge Canada/parts

From 2013hs.igem.org

(Difference between revisions)
(Blanked the page)
Line 1: Line 1:
-
 
+
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Strict//EN"
 +
"http://www.w3.org/TR/xhtml1/DTD/xhtml1-strict.dtd">
 +
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">
 +
<head>
 +
<meta http-equiv="content-type" content="text/html; charset=UTF-8" />
 +
<meta name="viewport" content="width=device-width" />
 +
<title>Parts</title>
 +
 +
<script src="http://code.jquery.com/jquery-1.9.1.min.js"></script>
 +
<script src="https://2013hs.igem.org/Team:Lethbridge_Canada/jquery.slides.min?action=raw&ctype=text/javascript"></script>
 +
<link rel="stylesheet" href="https://2013hs.igem.org/Team:Lethbridge_Canada/bootstrap?action=raw&ctype=text/css" />
 +
<link rel="stylesheet" href="https://2013hs.igem.org/Team:Lethbridge_Canada/concept?action=raw&ctype=text/css" type="text/css" media="screen" />
 +
<script type="text/javascript" src="https://2013hs.igem.org/Team:Lethbridge_Canada/sort_table1?action=raw&ctype=text/javascript"></script>
 +
  <script type="text/javascript" src="https://2013hs.igem.org/Team:Lethbridge_Canada/sort_table2?action=raw&ctype=text/javascript"></script>
 +
<link rel="stylesheet" type="text/css" href="https://2013hs.igem.org/Team:Lethbridge_Canada/sort_table?action=raw&ctype=text/css" />
 +
 +
</head>
 +
<body>
 +
<div id="parts">
 +
<div id="wrapper">
 +
<div id="header">
 +
 +
<a href="index.html">
 +
<div id="logo_all">
 +
<img src="logo.png" alt="logo" />
 +
</div>
 +
<span id="title_first">Lethbridge</span>
 +
<span id="title_second">iGEM Team</span>
 +
</a>
 +
</div>
 +
 +
<div id="content">
 +
 +
<div class="cleafix>">
 +
<div id="navigation">
 +
<ul>
 +
<li class="navigation_project"><a href="#">Project</a>
 +
<ul>
 +
<li></li>
 +
</ul>
 +
</li>
 +
<li class="navigation_parts"><a href="parts.html">Parts</a>
 +
<ul>
 +
<li></li>
 +
</ul>
 +
</li>
 +
<li class="navigation_notebook"><a href="protocols.html">Notebook</a></li>
 +
<li class="navigation_safety"><a href="safety.html">Safety</a></li>
 +
<li class="navigation_outreach"><a href="#">Outreach</a></li>
 +
<li class="navigation_team"><a href="team.html">Team</a></li>
 +
<li class="navigation_attributions"><a href="#">Sponsors</a></li>
 +
</ul>
 +
</div>
 +
</div>
 +
 +
 +
<div id="mainContent">
 +
 +
<div id="parts_section">
 +
 
 +
  <h2 class="table_title">Parts:</h2>
 +
 
 +
  <table class="sortable" id="sorter">
 +
 
 +
  <tr>
 +
<th> </th>
 +
<th> </th>
 +
<th> </th>
 +
<th>Name</th>
 +
<th>Type</th>
 +
<th>Description</th>
 +
<th>Designer(s)</th>
 +
<th>Length</th>
 +
</tr>
 +
 +
<tr>
 +
<td>&#9829;</td>
 +
<td>&#9733;</td>
 +
<td>W</td>
 +
<td><a class="part" href="http://partsregistry.org/Part:BBa_J23100">BBa_J23100</a></td>
 +
<td>Regulatory</td>
 +
<td>High constitutive promoter</td>
 +
<td>John Anderson</td>
 +
<td>35</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&#9829;</td>
 +
<td>&#9733;</td>
 +
<td>W</td>
 +
<td><a class="part" href="http://partsregistry.org/Part:BBa_J23108">BBa_J23108</a></td>
 +
<td>Regulatory</td>
 +
<td>Medium-high constitutive promoter</td>
 +
<td>John Anderson</td>
 +
<td>35</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&#9829;</td>
 +
<td>&#9733;</td>
 +
<td>W</td>
 +
<td><a class="part" href="http://partsregistry.org/Part:BBa_J23113">BBa_J23113</a></td>
 +
<td>Regulatory</td>
 +
<td>Medium-low constitutive promoter</td>
 +
<td>John Anderson</td>
 +
<td>35</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&#9829;</td>
 +
<td>&#9733;</td>
 +
<td>W</td>
 +
<td><a class="part" href="http://partsregistry.org/Part:BBa_J23114">BBa_J23114</a></td>
 +
<td>Regulatory</td>
 +
<td>Low constitutive promoter</td>
 +
<td>John Anderson</td>
 +
<td>35</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&#9829;</td>
 +
<td>&#9733;</td>
 +
<td>W</td>
 +
<td><a class="part" href="http://partsregistry.org/Part:BBa_J61002">BBa_J61002</a></td>
 +
<td>Plasmid_Backbone</td>
 +
<td>EX-Ptet-S-rbs-RFP-P “RFP reporter”</td>
 +
<td>John Anderson</td>
 +
<td>2948</td>
 +
</tr>
 +
 +
<tr>
 +
<td> </td>
 +
<td>&#9733;</td>
 +
<td>W</td>
 +
<td><a class="part" href="http://partsregistry.org/Part:BBa_B0034">BBa_B0034</a></td>
 +
<td>RBS</td>
 +
<td>RBS (Elowitz 1999) defines RBS efficiency</td>
 +
<td>Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.</td>
 +
<td>12</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&#9829;</td>
 +
<td> </td>
 +
<td>W</td>
 +
<td><a class="part" href="http://partsregistry.org/Part:BBa_K314100">BBa_K314100</a></td>
 +
<td>Composite</td>
 +
<td>J23100 promoter and B0034 RBS - High constitutive expression cassette</td>
 +
<td>Thomas Huber, Justin Siegel</td>
 +
<td>518</td>
 +
</tr>
 +
 +
<tr>
 +
<td> </td>
 +
<td>&#9733;</td>
 +
<td>W</td>
 +
<td><a class="part" href="http://partsregistry.org/Part:pSB1C3">pSB1C3</a></td>
 +
<td>Plasmid_Backbone</td>
 +
<td>High copy BioBrick assembly plasmid</td>
 +
<td>Austin Che</td>
 +
<td>2070</td>
 +
</tr>
 +
 +
<tr>
 +
<td> </td>
 +
<td> </td>
 +
<td> </td>
 +
<td>Oxytocin-NeurophysinI_pUC57</td>
 +
<td>Coding</td>
 +
<td>Protein hormone and carrier molecule for expression in pUC57 plasmid</td>
 +
<td>Lethbridge High School iGEM</td>
 +
<td>406</td>
 +
</tr>
 +
 +
<tr>
 +
<td> </td>
 +
<td> </td>
 +
<td> </td>
 +
<td>NECI_pUC57</td>
 +
<td>Coding</td>
 +
<td>Cleavage enzyme for expression in pUC57 plasmid</td>
 +
<td>Lethbridge High School iGEM</td>
 +
<td>2277</td>
 +
</tr>
 +
 +
<tr>
 +
<td> </td>
 +
<td>&#9733;</td>
 +
<td> </td>
 +
<td><a class="part" href="http://partsregistry.org/Part:BBa_J06702">BBa_J06702</a></td>
 +
<td>Reporter</td>
 +
<td>mCherry, bacterial with RBS and forward terminator </td>
 +
<td>Yves Wang</td>
 +
<td>869</td>
 +
</tr>
 +
 +
</table>
 +
 
 +
<script type="text/javascript">
 +
var sorter=new table.sorter("sorter");
 +
sorter.init("sorter",1);
 +
</script>
 +
</div>
 +
 
 +
<div id="constructs_section">
 +
 +
<h2 class="table_title">Final Constructs:</h2>
 +
 
 +
  <table class="sortable" id="sorter2">
 +
 
 +
<tr>
 +
    <th>Name</th>
 +
    <th>Description</th>
 +
    <th class="nosort">Expanded Length</th>
 +
    <th>Total Length</th>
 +
  </tr>
 +
   
 +
  <tr>
 +
<td>K314100+oxytocin-neurophysin1 in pSB1C3</td>
 +
<td>BBa_K314100 (J23100 promoter and B0034 RBS) and oxytocin-neurophysin1 gene in pSB1C3 plasmid</td>
 +
<td>518 + 406 + 2070</td>
 +
<td>2994</td>
 +
</tr>
 +
   
 +
<tr>
 +
    <td>K314100+NEC1 in pSB1C3</td>
 +
    <td>BBa_K314100 (J23100 promoter and B0034 RBS) and NEC1 enzyme in pSB1C3 plasmid</td>
 +
    <td>518 + 2277 + 2070</td>
 +
    <td>4865</td>
 +
    </tr>
 +
   
 +
    <tr>
 +
  <td>J23100+J06702 in pSB1C3</td>
 +
  <td>BBa_J23100 (high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid</td>
 +
  <td>35 + 869 + 2070</td>
 +
  <td>2974</td>
 +
</tr>
 +
   
 +
<tr>
 +
    <td>J23108+J06702 in pSB1C3</td>
 +
    <td>BBa_J23108 (medium-high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid</td>
 +
    <td>35 + 869 + 2070</td>
 +
    <td>2974</td>
 +
    </tr>
 +
   
 +
    <tr>
 +
    <td>J23113+J06702 in pSB1C3</td>
 +
    <td>BBa_J23113 (medium-low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid</td>
 +
    <td>35 + 869 + 2070</td>
 +
    <td>2974</td>
 +
    </tr>
 +
   
 +
    <tr>
 +
    <td>J23114+J06702 in pSB1C3</td>
 +
    <td>BBa_J23114 (low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid</td>
 +
    <td>35 + 869 + 2070</td>
 +
    <td>2974</td>
 +
    </tr>
 +
   
 +
    </table>
 +
 
 +
    <script type="text/javascript">
 +
var sorter2=new table.sorter("sorter2");
 +
sorter2.init("sorter2",1);
 +
</script>
 +
</div>
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</body>
 +
</html>

Revision as of 01:01, 4 June 2013

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Strict//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-strict.dtd"> Parts

Parts:

Name Type Description Designer(s) Length
♥ ★ W BBa_J23100 Regulatory High constitutive promoter John Anderson 35
♥ ★ W BBa_J23108 Regulatory Medium-high constitutive promoter John Anderson 35
♥ ★ W BBa_J23113 Regulatory Medium-low constitutive promoter John Anderson 35
♥ ★ W BBa_J23114 Regulatory Low constitutive promoter John Anderson 35
♥ ★ W BBa_J61002 Plasmid_Backbone EX-Ptet-S-rbs-RFP-P “RFP reporter” John Anderson 2948
★ W BBa_B0034 RBS RBS (Elowitz 1999) defines RBS efficiency Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 12
♥ W BBa_K314100 Composite J23100 promoter and B0034 RBS - High constitutive expression cassette Thomas Huber, Justin Siegel 518
★ W pSB1C3 Plasmid_Backbone High copy BioBrick assembly plasmid Austin Che 2070
Oxytocin-NeurophysinI_pUC57 Coding Protein hormone and carrier molecule for expression in pUC57 plasmid Lethbridge High School iGEM 406
NECI_pUC57 Coding Cleavage enzyme for expression in pUC57 plasmid Lethbridge High School iGEM 2277
★ BBa_J06702 Reporter mCherry, bacterial with RBS and forward terminator Yves Wang 869

Final Constructs:

Name Description Expanded Length Total Length
K314100+oxytocin-neurophysin1 in pSB1C3 BBa_K314100 (J23100 promoter and B0034 RBS) and oxytocin-neurophysin1 gene in pSB1C3 plasmid 518 + 406 + 2070 2994
K314100+NEC1 in pSB1C3 BBa_K314100 (J23100 promoter and B0034 RBS) and NEC1 enzyme in pSB1C3 plasmid 518 + 2277 + 2070 4865
J23100+J06702 in pSB1C3 BBa_J23100 (high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid 35 + 869 + 2070 2974
J23108+J06702 in pSB1C3 BBa_J23108 (medium-high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid 35 + 869 + 2070 2974
J23113+J06702 in pSB1C3 BBa_J23113 (medium-low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid 35 + 869 + 2070 2974
J23114+J06702 in pSB1C3 BBa_J23114 (low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid 35 + 869 + 2070 2974

Retrieved from "http://2013hs.igem.org/Team:Lethbridge_Canada/parts"