Team:The Agency Escondido/project/scissors

From 2013hs.igem.org

(Difference between revisions)
(Created page with "<html> <style> #top-section #p-logo{display:none;} #ocontent,#header{ width:100%; background-color: white...")
Line 191: Line 191:
                 #topheader-inner{
                 #topheader-inner{
                     width:975px;
                     width:975px;
-
                     margin-left: auto;
+
                     margin-left:auto;
                     margin-right:auto;
                     margin-right:auto;
                 }
                 }
-
                 #sections ul li {
+
                 #nav li {
                     display:inline;
                     display:inline;
                     margin:5px;
                     margin:5px;
Line 200: Line 200:
                     font-weight:bold;
                     font-weight:bold;
                 }
                 }
-
                 #sections ul li a{color:white;}
+
                 #nav li a{color:white;}
                 body{
                 body{
                     overflow-y: auto;
                     overflow-y: auto;
                 }
                 }
-
                 #sections ul{
+
                 #nav{
                     width:auto;
                     width:auto;
 +
                    float:left;
                 }
                 }
-
                 #sections{
+
                 #nav ul{
 +
                    background:rgba(255,255,255,0);
 +
                    list-style:none;
 +
                    position:absolute;
 +
                    left:-9999px;
 +
                }
 +
                #nav{
                     margin-left:240px;
                     margin-left:240px;
                     margin-right:-70px;
                     margin-right:-70px;
Line 214: Line 221:
                     overflow-x:visible;
                     overflow-x:visible;
                 }
                 }
-
                 #sections #selectedsection{
+
                 #nav li{
-
                     color: white;
+
                    float:left;
-
                     border-bottom:2px solid white;
+
                    margin-right:10px;
 +
                    position:relative;
 +
                }
 +
                #nav a:hover{
 +
                     color:#fff;
 +
                     background:#6b0c36;
 +
                    text-decoration:underline;
 +
                }
 +
                #nav li:hover ul{
 +
                    left:-30px;
 +
                }
 +
                #nav ul li{
 +
                    padding-top:10px;
 +
                    float:none;
 +
                }
 +
                #nav ul a{
 +
                    white-space:nowrap;
 +
                }
 +
                #nav li:hover a{
 +
                    background:rgba(0,0,0,1);
 +
                    text-decoration:none;
 +
                }
 +
                #nav li ul li a:hover{
 +
                    background:rgba(0,0,0,1);
 +
                    left:-30px;
                 }
                 }
                 table{
                 table{
Line 235: Line 266:
                     margin-bottom:10px;
                     margin-bottom:10px;
                 }
                 }
 +
               
             </style>
             </style>
         <div id="topheader">
         <div id="topheader">
Line 256: Line 288:
     <div id="ocontent-inner2">
     <div id="ocontent-inner2">
         <div id="sections">
         <div id="sections">
-
<ul>
+
<ul id="nav">
-
<li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido">About</a></li>
+
<li>
-
<li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido/notebook">Notebook</a></li>
+
    <a href="https://2013hs.igem.org/Team:The_Agency_Escondido">About</a>
-
<li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido/safety">Safety</a></li>
+
    <ul>
-
<li id="selectedsection">Project</a></li>
+
        <li></li>
-
<li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido/sandiego">San Diego</a></li>
+
    </ul>
-
<li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido/ourteam">Our Team</a></li>
+
</li>
 +
<li>
 +
    <a href="https://2013hs.igem.org/Team:The_Agency_Escondido/notebook">Notebook</a>
 +
    <ul>
 +
        <li></li>
 +
    </ul>
 +
</li>
 +
<li>
 +
    <a href="https://2013hs.igem.org/Team:The_Agency_Escondido/safety">Safety</a>
 +
    <ul>
 +
        <li></li>
 +
    </ul>
 +
</li>
 +
<li>
 +
    <a href="https://2013hs.igem.org/Team:The_Agency_Escondido/project"><u>Project</u></a>
 +
    <ul>
 +
        <li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido/project">Overveiw</a></li>
 +
        <li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido/project/rock">Rock</a></li>
 +
        <li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido/project/paper">Paper</a></li>
 +
        <li><a href="https://2013hs.igem.org/Team:The_Agency_Escondido/project/scissors">Scissors</a></li>
 +
    </ul>
 +
</li>
 +
<li>
 +
    <a href="https://2013hs.igem.org/Team:The_Agency_Escondido/sandiego">San Diego</a>
 +
    <ul>
 +
        <li></li>
 +
    </ul>
 +
</li>
 +
<li>
 +
    <a href="https://2013hs.igem.org/Team:The_Agency_Escondido/ourteam">Our Team</a>
 +
    <ul>
 +
        <li></li>
 +
    </ul>
 +
</li>
</ul>
</ul>
           </div>
           </div>
Line 271: Line 336:
Our project seeks to create two identical triads of transparent bacteria strains with complementary hydrophobic inducer production to emulate the game of "rock, paper, scissors" (Hydrophobic proteins can move through plasma membranes). Each triad will correspond to a group: group A and group B. Each type of bacteria will produce a specific protein that will act as an inducer to one and only one other bacteria strain(rock strain, paper strain, and scissors strain). Each triad will have different reporter genes designating its champion color. When two bacteria strains are combined in an agar plate, each protein secreted by the victorious strain, when acting as an inducer, changes the color of the cells on the plate identifying the champion.
Our project seeks to create two identical triads of transparent bacteria strains with complementary hydrophobic inducer production to emulate the game of "rock, paper, scissors" (Hydrophobic proteins can move through plasma membranes). Each triad will correspond to a group: group A and group B. Each type of bacteria will produce a specific protein that will act as an inducer to one and only one other bacteria strain(rock strain, paper strain, and scissors strain). Each triad will have different reporter genes designating its champion color. When two bacteria strains are combined in an agar plate, each protein secreted by the victorious strain, when acting as an inducer, changes the color of the cells on the plate identifying the champion.
</p>
</p>
 +
<p></p>
 +
<p></p>
 +
<img height="650px" width="700px" src=https://static.igem.org/mediawiki/2013hs/a/af/The_agency_escondido_plasmid_map_2013_2.gif></img>
 +
<a id="rock"></a>
 +
 +
<table align="center" border="0">
 +
  <tr>
 +
    <th><h2>Contents</h2></th>
 +
  </tr> 
 +
  <tr>
 +
      <td><a href="#rock"> Rock </a> </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="#paper"> Paper </a> </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="#scissors" > Scissors </a> </td> 
 +
  </tr>
 +
</table>
 +
<a id="scissors"></a>
<h2> Scissors </h2>  
<h2> Scissors </h2>  
Line 292: Line 377:
<h4>May 31</h4><p>Vansh Singh's plates showed no growth.</p>
<h4>May 31</h4><p>Vansh Singh's plates showed no growth.</p>
 +
 +
<h4>June 3</h4><p>Vansh Singh doubted his ligation and made another batch of Part X plasmids.</p>
 +
 +
<h4>June 4</h4><p>Vansh Singh and Evan Santos started the protocol for calcium chloride competent cells. The cells were incubated on ice over the night.</p>
 +
 +
<h4>June 5</h4><p>Vansh Singh and Evan Santos finished the calcium chloride competent cell protocol and transformed Part X with the competent cells. Because of the lack of LB agar plates, he left a 1.5ml eppendorf tube with the transformed cells and LB + chloremphenicol in the dry bath set at 37 degrees Celsius.</p>
</div>
</div>

Revision as of 00:27, 7 June 2013