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| - | <span id="title_first">Lethbridge</span>
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| - | <span id="title_second">iGEM Team</span>
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| - | <li class="navigation_project"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/project">Project</a>
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| - | <div id="mainContent">
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| - | <div id="notebook_header">
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| - | <div id="notebook_links">
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| - | <h1 id="notebook_links_header">Notebook Links</h1>
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| - | <ul id="notebook_march_links">
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| - | <li class="notebook_link_title"><a href="notebook_march.html">March</a></li>
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| - | <li><a href="notebook_march.html#entry_one">March 12, 2013: Transformation</a></li>
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| - | <li><a href="notebook_march.html#entry_two">March 13, 2013: Picking Cell Colonies (Cultures)</a></li>
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| - | <li><a href="notebook_march.html#entry_three">March 13, 2013: Miniprep and Glycerol Stock</a></li>
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| - | <li><a href="notebook_march.html#entry_four">March 15, 2013: Restriction Digestion</a></li>
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| - | <li><a href="notebook_march.html#entry_five">March 16, 2013: Agarose Gel</a></li>
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| - | <li><a href="notebook_march.html#entry_six">March 18, 2013: Agarose Gel</a></li>
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| - | <li><a href="notebook_march.html#entry_seven">March 19, 2013: Transformation</a></li>
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| - | <li><a href="notebook_march.html#entry_eight">March 20, 2013: Picking Cell Colonies (Cultures)</a></li>
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| - | <li><a href="notebook_march.html#entry_nine">March 21, 2013: Miniprep and Glycerol Stock</a></li>
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| - | <li><a href="notebook_march.html#entry_ten">March 27, 2013: Transformation</a></li>
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| - | <li><a href="notebook_march.html#entry_eleven">March 28, 2013: Transformation</a></li>
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| - | <li><a href="notebook_march.html#entry_twelve">March 28, 2013: Picking Cell Colonies (Cultures)</a></li>
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| - | <li><a href="notebook_march.html#entry_thirteen">March 29, 2013: Picking Cell Colonies (Cultures)</a></li>
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| - | <li><a href="notebook_march.html#entry_fourteen">March 29, 2013: Picking Cells of -80 Glycerol Stock</a></li>
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| - | </ul>
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| - | <ul id="notebook_april_links">
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| - | <li class="notebook_link_title"><a href="notebook_april.html">April</a></li>
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| - | <li><a href="notebook_april.html#entry_one">April 2, 2013: Restriction - Ligation</a></li>
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| - | <li><a href="notebook_april.html#entry_two">April 2, 2013: Ligation</a></li>
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| - | <li><a href="notebook_april.html#entry_three">April 3, 2013: Transformation</a></li>
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| - | <li><a href="notebook_april.html#entry_four">April 4, 2013: Re-Plating Transformation</a></li>
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| - | <li><a href="notebook_april.html#entry_five">April 5, 2013: Miniprep of ON Cultures</a></li>
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| - | <li><a href="notebook_april.html#entry_six">April 5, 2013: UV Spectroscopy DNA Concentrations</a></li>
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| - | <li><a href="notebook_april.html#entry_seven">April 6, 2013: Miniprep From Ligations</a></li>
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| - | <li><a href="notebook_april.html#entry_eight">April 7, 2013: PCR</a></li>
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| - | <li><a href="notebook_april.html#entry_nine">April 8, 2013: Restriction Digestion of PCR Parts</a></li>
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| - | <li><a href="notebook_april.html#entry_ten">April 8, 2013: 3% Agarose Gel of Digested PCR Parts</a></li>
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| - | <li><a href="notebook_april.html#entry_eleven">April 8, 2013: Gel Extraction</a></li>
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| - | <li><a href="notebook_april.html#entry_twelve">April 9, 2013: Repeat PCR</a></li>
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| - | <li><a href="notebook_april.html#entry_thirteen">April 9, 2013: Picking Cell Colonies (Cultures)</a></li>
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| - | <li><a href="notebook_april.html#entry_fourteen">April 10, 2013: Miniprep</a></li>
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| - | <li><a href="notebook_april.html#entry_fifteen">April 10, 2013: Restriction</a></li>
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| - | <li><a href="notebook_april.html#entry_sixteen">April 10, 2013: Agarose Gel of PCR 1%</a></li>
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| - | <li><a href="notebook_april.html#entry_seventeen">April 11, 2013: Miniprep</a></li>
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| - | <li><a href="notebook_april.html#entry_eighteen">April 12, 2013: Restriction Digestion</a></li>
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| - | <li><a href="notebook_april.html#entry_nineteen">April 12, 2013: PCR and Cell Reculturization</a></li>
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| - | <li><a href="notebook_april.html#entry_twenty">April 12, 2013: Miniprep</a></li>
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| - | <li><a href="notebook_april.html#entry_twenty_one">April 14, 2013: Miniprep</a></li>
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| - | <li><a href="notebook_april.html#entry_twenty_two">April 14, 2013: 1% Agarose Gel</a></li>
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| - | <li><a href="notebook_april.html#entry_twenty_six">April 15, 2013: Restriction/Digestion</a></li>
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| - | <li><a href="notebook_april.html#entry_twenty_seven">April 15, 2013: Restriction/Digestion</a></li>
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| - | <li><a href="notebook_april.html#entry_twenty_three">April 15, 2013: 1% Agarose Gel of Promoters</a></li>
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| - | <li><a href="notebook_april.html#entry_twenty_four">April 17, 2013: Transformation</a></li>
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| - | <li><a href="notebook_april.html#entry_twenty_five">April 30, 2013: 1% Agarose Gel of Promoter Constructs</a></li>
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| - | </ul>
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| - |
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| - | <ul id="notebook_may_links">
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| - | <li class="notebook_link_title"><a href="notebook_may.html">May</a></li>
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| - | <li><a href="notebook_may.html#entry_one">May 1, 2013: Ligation</a></li>
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| - | <li><a href="notebook_may.html#entry_two">May 4, 2013: Restriction Digest</a></li>
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| - | <li><a href="notebook_may.html#entry_three">May 4, 2013: 1% Agarose Gel of Promoter Constructs</a></li>
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| - | <li><a href="notebook_may.html#entry_four">May 4, 2013: Gel Extractions</a></li>
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| - | <li><a href="notebook_may.html#entry_five">May 5, 2013: Transformation</a></li>
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| - | <li><a href="notebook_may.html#entry_six">May 7, 2013: Restriction</a></li>
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| - | <li><a href="notebook_may.html#entry_seven">May 7, 2013: Gel Extraction</a></li>
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| - | <li><a href="notebook_may.html#entry_eight">May 7, 2013: Gel Extracted on Conformation Gel</a></li>
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| - | <li><a href="notebook_may.html#entry_nine">May 8, 2013: Ligations</a></li>
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| - | <li><a href="notebook_may.html#entry_ten">May 9, 2013: PCR of Ligation Mixture</a></li>
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| - | <li><a href="notebook_may.html#entry_eleven">May 13, 2013: Restriction of PCR Ligations Gel Conformation</a></li>
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| - | <li><a href="notebook_may.html#entry_twelve">May 14, 2013: Transformation of Ligations</a></li>
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| - | <li><a href="notebook_may.html#entry_thirteen">May 15, 2013: Ligation</a></li>
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| - | <li><a href="notebook_may.html#entry_fourteen">May 15, 2013: Picking Cells</a></li>
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| - | <li><a href="notebook_may.html#entry_fifteen">May 16, 2013: Picking Cells</a></li>
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| - | <li><a href="notebook_may.html#entry_sixteen">May 16, 2013: 10 Fold Miniprep</a></li>
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| - | <li><a href="notebook_may.html#entry_seventeen">May 17, 2013: Agarose Gel</a></li>
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| - | <li><a href="notebook_may.html#entry_eighteen">May 17, 2013: Miniprep</a></li>
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| - | <li><a href="notebook_may.html#entry_nineteen">May 17, 2013: Glycerol Stock</a></li>
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| - | </ul>
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| - | </div>
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| - | </div>
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| - |
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| - | <div id="notebook_body">
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| - | <div class="notebook_entry">
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| - | <div id="entry_one">
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| - | <h1 class="notebook_entry_header">Ligations</h1>
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| - | <ul class="notebook_entry_list">
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| - | <li>Name: Yoyo Yao</li>
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| - | <li>Date: May 1, 2013</li>
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| - | <li class="notebook_nested_start">Ligation of Parts – Upstream, Ligated at sites S & P:
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| - | <ul class="notebook_nested_list">
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| - | <li>J23100</li>
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| - | <li>J23113</li>
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| - | <li>J23108 +</li>
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| - | <li>J23108 –</li>
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| - | <li>All promoters already attached to Destination Plasmid J61002</li>
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| - | </ul>
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| - | <li class="notebook_nested_start">Downstream, Ligated at sites X & P</li>
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| - | <ul class="notebook_nested_list">
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| - | <li>J23100</li>
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| - | </ul>
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| - | </li>
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| - | </ul>
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| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
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| - | </div>
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| - | </div>
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| - |
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| - | <div class="notebook_entry">
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| - | <div id="entry_two">
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| - | <h1 class="notebook_entry_header">Restriction Digest</h1>
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| - | <div class="restrict_width">
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| - | <ul class="notebook_entry_list">
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| - | <li>Name: Mackenzie</li>
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| - | <li>Date: May 4, 2013</li>
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| - | <li>35µL DNA, 14µL MM, 0.5µL Spe1, 0.5µL Pst1</li>
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| - | </ul>
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| - | </div>
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| - | <ul>
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| - | <li>
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| - | <table class="table_two table_position">
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| - | <thead>
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| - | <tr>
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| - | <th colspan="2">Master Mix</th>
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| - | </tr>
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| - | </thead>
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| - | <tbody>
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| - | <tr>
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| - | <td>Milliq</td>
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| - | <td>36µl</td>
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| - | </tr>
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| - | <tr>
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| - | <td>BSA</td>
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| - | <td>45µl</td>
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| - | </tr>
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| - | <tr>
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| - | <td>10x buffer 2 NEB</td>
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| - | <td>45µl</td>
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| - | </tr>
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| - | </tbody>
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| - | </table>
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| - | </li>
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| - | </ul>
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| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
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| - | </div>
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| - | </div>
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| - |
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| - | <div class="notebook_entry">
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| - | <div id="entry_three">
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| - | <div class="restrict_width">
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| - | <h1 class="notebook_entry_header">1% Agarose Gel of Promoter Constructs</h1>
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| - | <ul class="notebook_entry_list">
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| - | <li>Name: Mackenzie</li>
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| - | <li>Date: May 4, 2013</li>
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| - | </ul>
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| - | </div>
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| - | <ul>
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| - | <li>
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| - | <table class="table_three table_position">
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| - | <thead>
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| - | <tr>
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| - | <th scope="col">Lane #</th>
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| - | <th scope="col">Contents</th>
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| - | <th scope="col">Volume</th>
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| - | </tr>
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| - | </thead>
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| - | <tbody>
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| - | <tr>
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| - | <td>1</td>
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| - | <td>1KB LADDER</td>
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| - | <td>6µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>2</td>
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| - | <td>J23100_J61002</td>
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| - | <td>25µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>3</td>
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| - | <td>J23100_J61002</td>
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| - | <td>25µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>4</td>
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| - | <td>J23100_J61002</td>
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| - | <td>25µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>5</td>
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| - | <td>J23100_J61002</td>
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| - | <td>25µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>6</td>
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| - | <td>J23108_J61002</td>
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| - | <td>25µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>7</td>
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| - | <td>J23108_J61002</td>
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| - | <td>25µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>8</td>
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| - | <td>J23108_J61002</td>
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| - | <td>25µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>9</td>
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| - | <td>J23108_J61002</td>
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| - | <td>25µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>10</td>
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| - | <td>J23113_J61002</td>
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| - | <td>25µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>11</td>
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| - | <td>J23113_J61002</td>
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| - | <td>25µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>12</td>
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| - | <td>J23113_J61002</td>
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| - | <td>25µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>13</td>
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| - | <td>J23113_J61002</td>
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| - | <td>25µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>14</td>
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| - | <td>J23114_J61002</td>
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| - | <td>25µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>15</td>
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| - | <td>J23114_J61002</td>
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| - | <td>25µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>16</td>
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| - | <td>J23114_J61002</td>
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| - | <td>25µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>17</td>
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| - | <td>J23114_J61002</td>
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| - | <td>25µL</td>
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| - | </tr>
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| - | </tbody>
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| - | </table>
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| - | </li>
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| - | </ul>
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| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
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| - | </div>
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| - | </div>
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| - |
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| - | <div class="notebook_entry">
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| - | <div id="entry_four">
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| - | <h1 class="notebook_entry_header">Gel Extraction</h1>
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| - | <ul class="notebook_entry_list">
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| - | <li>Date: May 4, 2013</li>
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| - | <li>J23108 J61002</li>
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| - | <li>J23113 J61002</li>
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| - | <li>J23114 J61002</li>
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| - | <li>J23100 J61002</li>
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| - | <li>Eluted 50 µL</li>
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| - | </ul>
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| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
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| - | </div>
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| - | </div>
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| - |
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| - | <div class="notebook_entry">
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| - | <div id="entry_five">
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| - | <h1 class="notebook_entry_header">Transformation</h1>
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| - | <div class="restrict_width_small">
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| - | <ul class="notebook_entry_list">
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| - | <li>Name: Mackenzie</li>
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| - | <li>Date: May 5, 2013</li>
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| - | </ul>
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| - | </div>
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| - | <ul>
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| - | <li>
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| - | <table class="table_four table_position">
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| - | <thead>
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| - | <tr>
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| - | <th scope="col">Tube #</th>
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| - | <th scope="col">Part #</th>
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| - | <th scope="col">Plasmid</th>
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| - | <th scope="col">Volume of Competent Cells</th>
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| - | </tr>
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| - | </thead>
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| - | <tbody>
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| - | <tr>
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| - | <td>1</td>
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| - | <td>J23100+J06702</td>
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| - | <td>J61002</td>
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| - | <td>20µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>2</td>
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| - | <td>J23108+J06702</td>
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| - | <td>J61002</td>
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| - | <td>20µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>3</td>
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| - | <td>J23113+J06702</td>
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| - | <td>J61002</td>
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| - | <td>20µL</td>
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| - | </tr>
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| - | <tr>
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| - | <td>4</td>
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| - | <td>J23108-J06702</td>
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| - | <td>J61002</td>
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| - | <td>20µL</td>
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| - | </tr>
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| - | </tbody>
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| - | </table>
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| - | </li>
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| - | </ul>
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| - |
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| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
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| - | </div>
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| - | </div>
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| - |
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| - | <div class="notebook_entry">
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| - | <div id="entry_six">
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| - | <h1 class="notebook_entry_header">Restriction</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li>Name: Chris Isaac</li>
| |
| - | <li>Date: May 7, 2013</li>
| |
| - | <li>Cut with ‘X’ and ‘P’</li>
| |
| - | <li>J06702 – RBS-mcherry-DT</li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
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| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_seven">
| |
| - | <h1 class="notebook_entry_header">Gel Extraction</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li>Name: Katie T. & Elaine B.</li>
| |
| - | <li>Date: May 7, 2013</li>
| |
| - | <li>J06702</li>
| |
| - | <li>Eluted at 30µL</li>
| |
| - | <li><img src="images/gel-extraction-05-07-2013.gif" alt="Gel Extraction Done on May 7, 2013" /></li>
| |
| - | </ul>
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| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
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| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_eight">
| |
| - | <div class="restrict_width">
| |
| - | <h1 class="notebook_entry_header">Gel Extracted Parts on Conformation Gel</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li>1µl of 1kb Ladder ng/0.5µg</li>
| |
| - | <li>2µl DNA in all samples</li>
| |
| - | <li class="move_up_table_two_long"><img src="images/gel-extracted-parts-on-conformation-gel.gif" alt="Gel Extracted Parts on Conformation Gel Image" /></li>
| |
| - | </ul>
| |
| - | </div>
| |
| - | <ul
| |
| - | <li>
| |
| - | <table class="table_two table_position">
| |
| - | <thead>
| |
| - | <tr>
| |
| - | <th>Lane #</th>
| |
| - | <th>Content</th>
| |
| - | </tr>
| |
| - | </thead>
| |
| - | <tbody>
| |
| - | <tr>
| |
| - | <td>1</td>
| |
| - | <td>Ladder</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>2</td>
| |
| - | <td>J06702</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>3</td>
| |
| - | <td>J23113–J61002 mc05/04/13mdy</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>4</td>
| |
| - | <td>J23108–J61002 mc05/04/13</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>5</td>
| |
| - | <td>J23100–J61002 mc05/04/13</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>6</td>
| |
| - | <td>J23114–J61002 mc05/04/13</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>7</td>
| |
| - | <td>Ladder</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>8</td>
| |
| - | <td>J23100–J 61002 EK150413dmy</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>9</td>
| |
| - | <td>J23108–J61002 EK150413</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>10</td>
| |
| - | <td>J23113–J61002EK150413</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>11</td>
| |
| - | <td>J23114 –J61002 EK150413</td>
| |
| - | </tr>
| |
| - | </tbody>
| |
| - | </table>
| |
| - | </li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_nine">
| |
| - | <h1 class="notebook_entry_header">Gel Extraction</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li>Name: Carissa K.</li>
| |
| - | <li>Date: May 8, 2013</li>
| |
| - | <li>2ul of J06702 mix with 4ul of each; J23100, J23108, J23113, J23114 all in plasmid J61002 (amp)</li>
| |
| - | <li>In the Thermo-cycler at 16 degrees Celsius overnight followed by heat kill.</li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_ten">
| |
| - | <h1 class="notebook_entry_header">PCR of Ligation Mixture(PCR to verify insert in clone vector)</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li>Name: Chris Isaac</li>
| |
| - | <li>Date: May 9, 2013</li>
| |
| - | <li>X7 Master Mix, Taq polymerase</li>
| |
| - | <li>J23100_J61002 (control)</li>
| |
| - | <li>J06702_pSB1AK2 (control)</li>
| |
| - | <li>J23100_J61002_J06702</li>
| |
| - | <li>J23108_J601002_J06702</li>
| |
| - | <li>J23113_J61002_J06702</li>
| |
| - | <li>J23114_J61002_J06702</li>
| |
| - |
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_eleven">
| |
| - | <h1 class="notebook_entry_header">Restriction of PCR Ligations Gel Conformation</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li>Name: Patrick O'</li>
| |
| - | <li>Date: May 13, 2013</li>
| |
| - | <li>Restriction of May 9th pcr ligation at E and P and ran on 1.0% Gel (refreshed ETH Bromide)</li>
| |
| - | <li>Ladder lane 2</li>
| |
| - | <li>2space</li>
| |
| - | <li>J06702_pSB1AK2 (control)</li>
| |
| - | <li>2spaces</li>
| |
| - | <li>J23100_J61002 (control)</li>
| |
| - | <li>spaces</li>
| |
| - | <li>J23100_J61002_J06702</li>
| |
| - | <li>J23108_J601002_J06702</li>
| |
| - | <li>J23113_J61002_J06702</li>
| |
| - | <li>J23114_J61002_J06702</li>
| |
| - | <li>Ladder</li>
| |
| - | <li>Conclusion: Ligations were not Successful</li>
| |
| - | <li><img src="images/gel-extraction-05-13-2013.gif" alt="Restriction of PCR Ligations Gel Conformation Image" /></li>
| |
| - |
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_twelve">
| |
| - | <h1 class="notebook_entry_header">Transformation of Ligations</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li>Date: May 14, 2013</li>
| |
| - | <li>Transformed ligation of J06702 with various promoters in plasmid J61002 : J23100, J23108, J23113, J23114 From May 8, 2013.</li>
| |
| - | <li>Transformed 3ul ligation mix into 30ul of Dh5 alpha competent cells, used puc19 control. Heat shocked for 30second at 42 degrees Celsius added 200ul warm LB, incubated at 37 for 1 hour, Plated 100ul on amp LB Agar plates. Overnight at 37degrees Celsius.</li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_thirteen">
| |
| - | <h1 class="notebook_entry_header">Ligation Round 2 of May 14th Gel Extracted Parts</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li>11ul dh20</li>
| |
| - | <li>2ul t4 dna ligase buffer</li>
| |
| - | <li>4ul DNA J06702</li>
| |
| - | <li>2ul DNA J23100, J23108, J23113, J23114 in plasmid J61002</li>
| |
| - | <li>1ul T4 dna Ligase</li>
| |
| - | <li>Into the Thermo-cycler overnight at 16 degrees Celsius.</li>
| |
| - |
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_fourteen">
| |
| - | <h1 class="notebook_entry_header">Picking Cells</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li>Name: Yoyo, Amrinder, Patrick, Orion</li>
| |
| - | <li>Date: May 15, 2013</li>
| |
| - | <li>Put 50µL of Amp resistance into 50mL of Lb media</li>
| |
| - | <li class="notebook_nested_start">Flasks:</li>
| |
| - | <li>
| |
| - | <ul class="notebook_nested_list">
| |
| - | <li>J23100_J61002</li>
| |
| - | <li>J23108_J61002</li>
| |
| - | <li>J23113_J61002</li>
| |
| - | <li>J23114_J61002</li>
| |
| - | <li>J06702_psb1ak2</li>
| |
| - | </ul>
| |
| - | </li>
| |
| - | <li>Inserted tips of E. Coli into each flask</li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_fifteen">
| |
| - | <h1 class="notebook_entry_header">Picking Cells</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li>Name: Amrinder, Orion</li>
| |
| - | <li>Date: May 16, 2013</li>
| |
| - | <li>Put 5µL of Amp into 5mL of Lb media</li>
| |
| - | <li class="notebook_nested_start">Flasks:</li>
| |
| - | <li>
| |
| - | <ul class="notebook_nested_list">
| |
| - | <li>J23100_J61002</li>
| |
| - | <li>J23108_J61002</li>
| |
| - | <li>J23113_J61002</li>
| |
| - | <li>J23114_J61002</li>
| |
| - | <li>J06702_psb1ak2</li>
| |
| - |
| |
| - | </ul>
| |
| - | </li>
| |
| - | <li>Inserted tips of E. Coli into each tube</li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_sixteen">
| |
| - | <h1 class="notebook_entry_header">10 Fold Miniprep</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li>Name: Isaac, Patrick</li>
| |
| - | <li>Date: May 16, 2013</li>
| |
| - | <li>Spun down 50ml of culture in falcon tubes</li>
| |
| - | <li>5000xg twice for 2mins to collect cells</li>
| |
| - | <li>Resupended in 1ml solution1 2ml solution 2 and 3,5ml solution 3</li>
| |
| - | <li>Spun down at 6000xg for 6minutes to pellet cell debries</li>
| |
| - | <li class="notebook_nested_start">Eluted into 4 miniprep spin collumns twice to reach maximum binding effeciency<li>
| |
| - | <li>
| |
| - | <ul class="notebook_nested_list">
| |
| - | <li>Combined eluted minipreps into one sample totalling 200µL</li>
| |
| - | </ul>
| |
| - | </li>
| |
| - |
| |
| - | <li class="notebook_nested_start">Mini prep of (50µL EB):</li>
| |
| - | <li>
| |
| - | <ul class="notebook_nested_list">
| |
| - | <li>J23100_J61002</li>
| |
| - | <li>J23108_J61002</li>
| |
| - | <li>J23113_J61002</li>
| |
| - | <li>J23114_J61002</li>
| |
| - | <li>J06702_PSB1AK2</li>
| |
| - | </ul>
| |
| - | </li>
| |
| - | <li>Mini-prepped with EZ-10 columns</li>
| |
| - | <li>Eluted 35µL</li>
| |
| - | <li>Purified DNA in Erin’s Box</li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_seventeen">
| |
| - | <h1 class="notebook_entry_header">Agarose Gel</h1>
| |
| - | <div class="restrict_width">
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li>Name: Krista Fjordbotten, Yoyo Yao</li>
| |
| - | <li>Date: May 17, 2013</li>
| |
| - | <li class="notebook_nested_start">Restriction of:</li>
| |
| - | <li>
| |
| - | <ul class="notebook_nested_list">
| |
| - | <li>4μL DNA</li>
| |
| - | <li>1μL Enzyme</li>
| |
| - | <li>2.5μL Buffer</li>
| |
| - | <li>17.5μL Water</li>
| |
| - | <li class="notebook_second_nested">J23100_J06702 J61002</li>
| |
| - | <li class="notebook_second_nested">J23108_J06702 J61002</li>
| |
| - | <li class="notebook_second_nested">J23113_J06702 J61002</li>
| |
| - | <li class="notebook_second_nested">J23114_J06702 J61002</li>
| |
| - | <li class="notebook_second_nested">J06702 J61002</li>
| |
| - | </ul>
| |
| - | </li>
| |
| - | <li>Ladder: 2μL Ladder, 0.5μL Dye</li>
| |
| - | <li><img src="images/conformation-gel-17-05-2013.gif" alt="Conformation Gel on May 17, 2013" /></li>
| |
| - | <li>Conformation of all 5 of May 16, 2013 minipreps</li>
| |
| - | </ul>
| |
| - | </div>
| |
| - | <ul>
| |
| - | <li>
| |
| - | <table class="table_three table_position">
| |
| - | <thead>
| |
| - | <tr>
| |
| - | <th>Well #(from left)</th>
| |
| - | <th>DNA Parts</th>
| |
| - | <th>Concentration</th>
| |
| - | </tr>
| |
| - | </thead>
| |
| - | <tbody>
| |
| - | <tr>
| |
| - | <td>2</td>
| |
| - | <td>ladder</td>
| |
| - | <td>2μL Ladder/0.5μL Dye</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>4</td>
| |
| - | <td>J23100_J06702 J61002</td>
| |
| - | <td>25μL DNA/5μL Dye</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>6</td>
| |
| - | <td>J23108_J06702 J61002</td>
| |
| - | <td>25μL DNA/5μL Dye</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>8</td>
| |
| - | <td>J23113_J06702 J61002</td>
| |
| - | <td>25μL DNA/5μL Dye</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>10</td>
| |
| - | <td>J23114_J06702 J61002</td>
| |
| - | <td>25μL DNA/5μL Dye</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>12</td>
| |
| - | <td>J06702 J61002</td>
| |
| - | <td>25μL DNA/5μL Dye</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>14</td>
| |
| - | <td>ladder</td>
| |
| - | <td>2μL Ladder/0.5μL Dye</td>
| |
| - | </tr>
| |
| - | </tbody>
| |
| - | </table>
| |
| - | </li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_eighteen">
| |
| - | <h1 class="notebook_entry_header">Miniprep</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li>Name: Amrinder Grewal , Krista Fjordbotten</li>
| |
| - | <li>Date: May 17, 2013</li>
| |
| - | <li class="notebook_nested_start">Mini prep of (35µL EB):</li>
| |
| - | <li>
| |
| - | <ul class="notebook_nested_list">
| |
| - | <li class="notebook_second_nested">J23100_J61002</li>
| |
| - | <li class="notebook_second_nested">J23108_J61002</li>
| |
| - | <li class="notebook_second_nested">J23113_J61002</li>
| |
| - | <li class="notebook_second_nested">J23114_J61002</li>
| |
| - | <li>Mini-prepped with EZ-10 columns</li>
| |
| - | <li>Eluted 35µL</li>
| |
| - | <li>Purified DNA in Erin’s Box</li>
| |
| - |
| |
| - | </ul>
| |
| - | </li>
| |
| - | <li>Inserted tips of E. Coli into each flask</li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_nineteen">
| |
| - | <h1 class="notebook_entry_header">Glycerol Stock</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li>Name: Amrinder Grewal , Krista Fjordbotten, Yoyo Yao, Isaac Ward</li>
| |
| - | <li>Date: May 17, 2013</li>
| |
| - | <li class="notebook_nested_start">Glycerol Stocked:</li>
| |
| - | <ul>
| |
| - | <li>J23100_J61002</li>
| |
| - | <li>J23108_J61002</li>
| |
| - | <li>J23113_J61002</li>
| |
| - | <li>J23114_J61002</li>
| |
| - | </ul>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | </div>
| |
| - | </div>
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| - | </div>
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