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Team:Beijing HDFLS High/Project
From 2013hs.igem.org
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== PARTS== | == PARTS== | ||
===Part A:BBa_J22106 rec A (SOS) Promoter === | ===Part A:BBa_J22106 rec A (SOS) Promoter === | ||
| - | Extensive UV irradiation impings on heavily DNA damage and results in a single-strand DNA occuring, which activates RecA protein.Then,RecA protein leads to the expression of about 20 different repair proteins to rescue DNA damage.RecA protein is the central regulator in SOS repair system.Rec A (SOS) Promoter | + | Extensive UV irradiation impings on heavily DNA damage and results in a single-strand DNA occuring, which activates RecA protein.Then,RecA protein leads to the expression of about 20 different repair proteins to rescue DNA damage.RecA protein is the central regulator in SOS repair system.Rec A (SOS) Promoter is available in 2004 registry part plate. |
===Part B:BBa_J45119 Wintergreen odor enzyme (BwMST1) generator=== | ===Part B:BBa_J45119 Wintergreen odor enzyme (BwMST1) generator=== | ||
Revision as of 21:00, 14 June 2013
Contents |
TITLE: The UV-responsive Wintergreen Odor Generator
ABSTRACT
The UV-responsive wintergreen odor generator catalyzes the conversion of the precursor salicylic acid to the wintergreen odor methyl salicylate under extensive UV irradiation. The biosynthetic device is composed of two transcriptional devices: Rec A (SOS) promoter (BBa_J22106) and a wintergreen odor enzyme generator (BBa_J45119). The odor generator takes as input extensive UV irradiation and produce as output an enzyme that catalyzes production of an odor from a chemical precursor.
INTRODUCTION
Recently, because the ozone layer has been destroying by some artificial chemical substances like CFC (Freon),the increase of UV irradiation appears on the earth. Extensive UV irradiation is harmful to human being, such as
- 1) Weaken the immune system of human
- 2) Increase the risk of skin cancer, cataracts
- 3) Make a heavy damage in genes
We designed a biological system in E.coli to detect extensive UV irradiation. If the toolkit is under extensive UV irradiation, it will produce a wintergreen odor.Then, This special odor will warn people of the UV irradiation. In order to increase the attraction of our product, we make E.coli culture medium with different color, using red, yellow and blue pigments.
PARTS
Part A:BBa_J22106 rec A (SOS) Promoter
Extensive UV irradiation impings on heavily DNA damage and results in a single-strand DNA occuring, which activates RecA protein.Then,RecA protein leads to the expression of about 20 different repair proteins to rescue DNA damage.RecA protein is the central regulator in SOS repair system.Rec A (SOS) Promoter is available in 2004 registry part plate.
Part B:BBa_J45119 Wintergreen odor enzyme (BwMST1) generator
BBa_J45119 takes as input a transcriptional signal (PoPS) and produce as output the BMST1 enzyme that catalyzes production of methyl salicylate from salicylic acid. Methyl salicylate has a wintergreen smell. Wintergreen odor enzyme (BMST1) generator is availabe in 2005 registry part plate.
RESULTS
Enzyme digestion of Part A and Part B
Enzyme digestion after ligation
STEP
STEP1: TRANSFORMATION AND GROWING
(Make sure we have enough copies of BBa_J22106 and BBa_J45119.)
STEP2: MINIPREP
STEP3：RESTRICTION DIGEST
STEP4：LIGATION
STEP5：TRANSFORMATION
STEP6：MINIPREP
STEP7：RESTRICTION DIGEST
STEP8：DNA SEQUENCING
STEP9：UV INDUCTION
STEP10：DESGIN AND COMPLETE
Presentation
We discussed the project by ourselves with several presentations, and also gave the presentation to all of the students at Grade 9 in order to attract and encourage them to participate in the HW iGEM team in the next year!
