Team:St Pauls London/Safety

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Revision as of 13:37, 17 June 2013

Safety

On top of all of the safety requirements and mitigation strategies discussed below, all lab-work conformed to and was carried out in accordance to the SPS safety requirements. All lab-work was supervised by members of staff and lab technicians at all times. The SPS lab safety policy is below:

1. Your own safety and that of others depends upon sensible behaviour in the laboratories. The Health and Safety at Work Act holds teachers and pupils responsible for their actions if others are harmed by them.

2. You should never run, push other people or throw anything when you are in a laboratory.

3. When you are doing practical work always work methodically, using care and common sense. Rushing leads to accidents.

4. If you are not sure of what you are supposed to do, ASK for help. Don't muddle on hopefully. Listen to, and act on, any verbal instructions your teacher may give.

5. You should only go into a laboratory if you have permission to do so.

6. Apparatus and chemicals may only be removed from a laboratory if you have permission to do so.

7. Don't touch apparatus, electrical equipment or chemicals (or the controls of the gas, water and electricity supplies) unless you have been told to do so.

8. If you have any doubts about the safety of any equipment you are expected to use e.g. a jagged piece of glassware, consult your teacher.

9. Report ANY accident, however slight, to your teacher.

10. Waste, or surplus, materials should be disposed of as instructed by your teacher.

DON'T throw solids into sinks.

    	::DON'T pour non-aqueous liquids into sinks unless told to do so.
    	::DON'T return chemicals or solutions to their original storage containers.

11. Don't put hot objects directly onto a wooden bench.

    	Make sure anything burning or smouldering e.g. a splint, is fully extinguished.
    	Allow hot equipment or substances to cool before you touch them, store them or throw them away.

12. Don't sit or lean on a laboratory bench, as you or your clothing may come into contact with harmful substances. If you spill any substance accidentally, clear it up after consulting your teacher.

13. Avoid contact of chemicals with your skin. Wash your hands after laboratory work; this is especially important before meals.

14. If chemicals or apparatus are left on the bench from the previous lesson do not touch them but immediately inform your teacher.

15. Always leave your bag in a safe place in the laboratory, so that it cannot cause an obstruction.

16. Wear the eye protection provided at all times during Biology and Chemistry practical work, and when you are told to do so in Physics.

17. Long hair must be tied back during practicals.

18. Ties and shirts should be tucked in during practicals.

19. Don't eat or drink in laboratories unless invited to do so by the teacher in charge.

20. You should know the meaning of the hazard warning symbols found on storage

      containers.

Letter Code for Part	BioBrick Reference	Function	Organism Derived From	Risk Group
A	BBa_K418003	Promoter	E. coli strain NEB 10-beta	1
B	BBa_B0033	RBS	E. coli strain NEB 10-beta	1
C	BBa_C0040	TetR gene	E. coli strain NEB 10-beta	1
D	BBa_C0072	Lacl gene	P22 bacteriophage	1
E	BBa_E1010	RFP gene	Discosoma striata	1
F	BBa_B0015	Terminator	E. coli strain NEB 10-beta	1
J	BBa_K091104	Promoter	E. coli strain NEB 10-beta	1
K	BBa_B0034	RBS	E. coli strain NEB 10-beta	1
L	BBa_E0030	YFP gene	Aequorea victoria	1

@@ Line 305: / Line 305: @@
 ===Safety===
-:1. All procedures were carried out in a clean area. This area was set up as detailed below, and ensured that no external microorganisms contaminated plates or tubes.
+'''On top of all of the safety requirements and mitigation strategies discussed below, all lab-work conformed to and was carried out in accordance to the SPS safety requirements.  All lab-work was supervised by members of staff and lab technicians at all times.  The SPS lab safety policy is below:'''
-::* Bunsen burners were on at all times, such that a updraft was always present.
-::* Prior to any procedures taking place, the entire area which was to be used was scrubbed down with antiseptic.
-:2. All waste was disposed of in separate, marked bins. The contents of these were then incinerated.
+:1.  	Your own safety and that of others depends upon sensible behaviour in the laboratories. The Health and Safety at Work Act holds teachers and pupils responsible for their actions if others are harmed by them.
-:3. Antimicrobial hand wash was systematically used upon entering and leaving the lab to ensure no genetically modified microorganisms could be released outside of the one projects labs in which procedures were carried out.
+:2.  	You should never run, push other people or throw anything when you are in a laboratory.
-:4. Sterile gloves were worn at all times.
+:3.  	When you are doing practical work always work methodically, using care and common sense. Rushing leads to accidents.
+:4.  	If you are not sure of what you are supposed to do, ASK for help. Don't muddle on hopefully. Listen to, and act on, any verbal instructions your teacher may give.
+:5.  	You should only go into a laboratory if you have permission to do so.
+:6.  	Apparatus and chemicals may only be removed from a laboratory if you have permission to do so.
+:7.  	Don't touch apparatus, electrical equipment or chemicals (or the controls of the gas, water and electricity supplies) unless you have been told to do so.
+:8.  	If you have any doubts about the safety of any equipment you are expected to use e.g. a jagged piece of glassware, consult your teacher.
+:9.  	Report ANY accident, however slight, to your teacher.
+:10.	Waste, or surplus, materials should be disposed of as instructed by your teacher.
+::DON'T throw solids into sinks.
+     	::DON'T pour non-aqueous liquids into sinks unless told to do so.
+     	::DON'T return chemicals or solutions to their original storage containers.
+:11.	Don't put hot objects directly onto a wooden bench.
+     	Make sure anything burning or smouldering e.g. a splint, is fully extinguished.
+     	Allow hot equipment or substances to cool before you touch them, store them or throw them away.
+:12.	Don't sit or lean on a laboratory bench, as you or your clothing may come into contact with harmful substances. If you spill any substance accidentally, clear it up after consulting your teacher.
+:13.	Avoid contact of chemicals with your skin. Wash your hands after laboratory work; this is especially important before meals.
+:14.    If chemicals or apparatus are left on the bench from the previous lesson do not touch them but immediately inform your teacher.
+:15.	Always leave your bag in a safe place in the laboratory, so that it cannot cause an obstruction.
+:16.	Wear the eye protection provided at all times during Biology and Chemistry practical work, and   when you are told to do so in Physics.
+:17.    Long hair must be tied back during practicals.
+:18.    Ties and shirts should be tucked in during practicals.
+:19.	Don't eat or drink in laboratories unless invited to do so by the teacher in charge.
+:20.   You should know the meaning of the hazard warning symbols found on storage
+       containers.
-:5. Safety glasses were worn at all times.
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