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Team:Shenzhen SFLS/Project
From 2013hs.igem.org
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===Project=== | ===Project=== | ||
Student at SFLS want to produce an enzyme known as “acid phosphatase” (“AP”). AP is known to be inhibited by heavy metals, and it might be inhibited by different pesticides. In order to attain AP, students could simply order it or extract it from a plant. For IGEM, however, they will need to do it the hard way: they will need to force a bacteria to produce more AP than it normally does. This can be achieved by: | Student at SFLS want to produce an enzyme known as “acid phosphatase” (“AP”). AP is known to be inhibited by heavy metals, and it might be inhibited by different pesticides. In order to attain AP, students could simply order it or extract it from a plant. For IGEM, however, they will need to do it the hard way: they will need to force a bacteria to produce more AP than it normally does. This can be achieved by: | ||
| + | |||
１．Find out what gene is responsible for AP production. Figure out if it can be easily inserted into e.coli or yeast genome | １．Find out what gene is responsible for AP production. Figure out if it can be easily inserted into e.coli or yeast genome | ||
| + | |||
２．Order this gene from a laboratory． | ２．Order this gene from a laboratory． | ||
| + | |||
３．Insert（插入） this gene into e。coil or yeast. | ３．Insert（插入） this gene into e。coil or yeast. | ||
| + | |||
４．Isolate（隔离） AP after it has been expressed. | ４．Isolate（隔离） AP after it has been expressed. | ||
| - | ５． Use fluorometric | + | |
| + | ５． Use fluorometric ａｎａｌｙｓｉｓ（荧光分析）ａｎｄ ｎｏｔｅ ｔｈｅ baseline ｗａｖｅｌｅｎｇｔｈ（排列波长） ｏｆ ｌｉｇｈｔ ｓｈｏｔ ｔｈｒｏｕｇｈ ａ agarose／jellose／ＡＰ in the presence of an acid. | ||
===Team=== | ===Team=== | ||
Revision as of 08:48, 25 March 2013
- a team description
- project description
- safety information (did your team take a safety training course? were you supervised in the lab?)
- team attribution (who did what part of your project?)
- lab notebook
- sponsor information
- other information
Example: 2013hs.igem.org/Team:Shenzhen_SFLS/Our_Pets
Project
Student at SFLS want to produce an enzyme known as “acid phosphatase” (“AP”). AP is known to be inhibited by heavy metals, and it might be inhibited by different pesticides. In order to attain AP, students could simply order it or extract it from a plant. For IGEM, however, they will need to do it the hard way: they will need to force a bacteria to produce more AP than it normally does. This can be achieved by:
１．Find out what gene is responsible for AP production. Figure out if it can be easily inserted into e.coli or yeast genome
２．Order this gene from a laboratory．
３．Insert（插入） this gene into e。coil or yeast.
４．Isolate（隔离） AP after it has been expressed.
５． Use fluorometric ａｎａｌｙｓｉｓ（荧光分析）ａｎｄ ｎｏｔｅ ｔｈｅ baseline ｗａｖｅｌｅｎｇｔｈ（排列波长） ｏｆ ｌｉｇｈｔ ｓｈｏｔ ｔｈｒｏｕｇｈ ａ agarose／jellose／ＡＰ in the presence of an acid.
Team
blablabla
Notebook
Show us how you spent your days.
Results/Conclusions
What did you achieve over the course of your semester?
Safety
What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
Attributions
Who worked on what?
Human Practices
What impact does/will your project have on the public?
Fun!
What was your favorite team snack?? Have a picture of your team mascot?
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