Team:Shenzhen SFLS/Project

From 2013hs.igem.org

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Revision as of 06:01, 26 March 2013

This is a template page. READ THESE INSTRUCTIONS.

You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.

You MUST have the following information on your wiki:

a team description
project description
safety information (did your team take a safety training course? were you supervised in the lab?)
team attribution (who did what part of your project?)

You may also wish to add other page such as:

lab notebook
sponsor information
other information

REMEMBER, keep all of your pages within your teams namespace.
Example: 2013hs.igem.org/Team:Deerfield_MA/Our_Pets

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)	Your team picture
	Team Shenzhen_SFLS

Official Team Profile

Student at SFLS want to produce an enzyme known as “acid phosphatase” (“AP”). AP is known to be inhibited by heavy metals, and it might be inhibited by different pesticides. In order to attain AP,　students could simply order it or extract it from a plant.　For IGEM, however, they will need to do it the hard way: they will need to force a bacteria to produce more AP than it normally does.　This can be achieved by:　

１．Find out what gene is responsible for AP production. Figure out if it can be easily inserted into e.coli or yeast　genome.

２．Order this　gene from a　laboratory．

３．Insert this gene into　e。coil　or yeast.

４．Isolate AP　after it has been expressed.

５． Use　fluorometric ａｎａｌｙｓｉｓａｎｄｎｏｔｅｔｈｅ baseline　ｗａｖｅｌｅｎｇｔｈoｆｌｉｇｈｔ　ｓｈｏｔ　ｔｈｒｏｕｇｈ　ａ　agarose／jellose／ＡＰ in the presence of an acid.

Add different heavy metals or pesticides to this mixture and do other fluorometric analyses. These analyses should yield different wavelengths because AP activity is inhibited by heavy metals, etc.

Notebook

Show us how you spent your days.

Results/Conclusions

What did you achieve over the course of your semester?

Safety

What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?

Attributions

Who worked on what?

Human Practices

What impact does/will your project have on the public?

Fun!

What was your favorite team snack?? Have a picture of your team mascot?

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 ４．Isolate AP　after it has been expressed.
-５． Use　fluorometric ａｎａｌｙｓｉｓ ａｎｄ ｎｏｔｅ ｔｈｅ baseline　ｗａｖｅｌｅｎｇｔｈ（排列波长） ｏｆ　ｌｉｇｈｔ　ｓｈｏｔ　ｔｈｒｏｕｇｈ　ａ　agarose／jellose／ＡＰ in the presence of an acid.
+５． Use　fluorometric ａｎａｌｙｓｉｓ ａｎｄ ｎｏｔｅ ｔｈｅ baseline　ｗａｖｅｌｅｎｇｔｈoｆｌｉｇｈｔ　ｓｈｏｔ　ｔｈｒｏｕｇｈ　ａ　agarose／jellose／ＡＰ in the presence of an acid.
 Add different heavy metals or pesticides to this mixture and do other fluorometric analyses. These analyses should yield different wavelengths because AP activity is inhibited by heavy metals, etc.
 ===Notebook===