Team:Deerfield MA

From 2013hs.igem.org

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Revision as of 17:03, 1 April 2013

This is a template page. READ THESE INSTRUCTIONS.

You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.

You MUST have the following information on your wiki:

a team description
project description
safety information (did your team take a safety training course? were you supervised in the lab?)
team attribution (who did what part of your project?)

You may also wish to add other page such as:

lab notebook
sponsor information
other information

REMEMBER, keep all of your pages within your teams namespace.
Example: 2013hs.igem.org/Team:Deerfield_MA/Our_Pets

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)	Your team picture
	Team Deerfield_MA

Official Team Profile

Mercury poisoning is a serious problem, which can devastate fish populations and ecosystems. Humans who consume contaminated fish may experience negative affects as a result. The Deerfield, MA iGEM Team has proposed a remedy to this issue with the aid of a biosensor. Our goal is to manipulate the system found in part K346001 that uses the merR gene to detect mercury, and then either promote or suppress a reporter gene. Previously GFP has been used as the reporter, but our plan is to test a few different reporters including Lumazine (K216007) and LacZ (I0500). We will test for transform efficiency, reporter strength, and objective overall succes to find which reporter is most effective in this system. Once these transformed cells are produced, someone (i.e. a fisherman) would be able to take a water sample in the area he or she plans to fish, allow the cells to inoculate overnight in the water sample, and then discover whether mercury is present in the water source. Depending on the output, the cells may be able to detect the severity of the contamination.

Notebook

Show us how you spent your days.

Results/Conclusions

What did you achieve over the course of your semester?

Safety

What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?

Attributions

Who worked on what?

Human Practices

What impact does/will your project have on the public?

Fun!

What was your favorite team snack?? Have a picture of your team mascot?

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-===Project===
+Mercury poisoning is a serious problem, which can devastate fish populations and ecosystems. Humans who consume contaminated fish may experience negative affects as a result. The Deerfield, MA iGEM Team has proposed a remedy to this issue with the aid of a biosensor. Our goal is to manipulate the system found in part K346001 that uses the merR gene to detect mercury, and then either promote or suppress a reporter gene. Previously GFP has been used as the reporter, but our plan is to test a few different reporters including Lumazine (K216007) and LacZ (I0500). We will test for transform efficiency, reporter strength, and objective overall succes to find which reporter is most effective in this system. Once these transformed cells are produced, someone (i.e. a fisherman) would be able to take a water sample in the area he or she plans to fish, allow the cells to inoculate overnight in the water sample, and then discover whether mercury is present in the water source. Depending on the output, the cells may be able to detect the severity of the contamination.
-What are you working on this semester?
 ===Notebook===