Team:The Agency Escondido

From 2013hs.igem.org

(Difference between revisions)

Revision as of 01:57, 4 April 2013

Team Description

The Agency is an organization of students, parents, faculty and mentors operating out of the Classical Academy High School of Escondido, CA. Our objective is to compete in challenging venues, and to foster the development of skills and character needed to each occasion. Our efforts are focused in areas of science, technology, engineering, and mathematics (STEM).

Project Description

Our project seeks to create two triads of transparent bacteria strains with complementary promoter production to emulate the game of "rock, paper, scissors". Each triad will correspond to a team: team 1 and team 2. Each type of bacteria will produce a specific protein that will act as a promoter to one and only one other bacteria strain(Rock strain, Paper strain, and Scissors strain). Each triad will have different reporter genes designating its champion color. When two bacteria strains are combined in an agar plate, each protein secreted by the victorious strain, when acting as a promoter, changes the color of the cells on the plate identifying the champion.

At the time of writing, the promoters to be used have not been selected, nor have the reporter genes been chosen.

Safety

Safety is our greatest concern. With copious amounts of reagents required for our projects, we created a web application to manage the material safety data sheets of reagents so that that they can be stored and accessed on a moments notice. Material safety data sheets are designed to raise awareness of substances and illuminate our members of their heterogeneous natures.

This page is not complete! Required remaining:

Attribution
Sponsors

@@ Line 194: / Line 194: @@
 </h2>
 <p>
-Our project seeks to create a triad of similar bacteria strains with complementary antibiotic production and antibiotic resistance to emulate the game of "rock, paper, scissors". Each type of bacteria will produce a single antibiotic while remaining resistant to both its own antibiotic and that of one other bacteria type. Each type will additionally resist an antibiotic included in an agar "playing field".</p>
+Our project seeks to create two triads of transparent bacteria strains with complementary promoter production to emulate the game of "rock, paper, scissors". Each triad will correspond to a team: team 1 and team 2. Each type of bacteria will produce a specific protein that will act as a promoter to one and only one other bacteria strain(Rock strain, Paper strain, and Scissors strain). Each triad will have different reporter genes designating its champion color. When two bacteria strains are combined in an agar plate, each protein secreted by the victorious strain, when acting as a promoter, changes the color of the cells on the plate identifying the champion.
-<p>When two of the three types of bacteria are combined and exposed to an environmental stimulus, one type will resist the antibiotic produced by the other, while the other will be vulnerable to the antibiotic produced by the first. The first type will therefore spread freely through the media while the growth of the second will be inhibited. To identify the "victorious" strain, each will include a different fluorescent reporter gene. </p>
+<!--When two of the three types of bacteria are combined and exposed to an environmental stimulus, one type will resist the antibiotic produced by the other, while the other will be vulnerable to the antibiotic produced by the first. The first type will therefore spread freely through the media while the growth of the second will be inhibited. To identify the "victorious" strain, each will include a different fluorescent reporter gene.--> </p>
-<p>At the time of writing, the antibiotics to be used have not been selected, nor have the reporter genes been chosen.</p>
+<p>At the time of writing, the promoters to be used have not been selected, nor have the reporter genes been chosen.</p>
 <h2>
 Safety
 </h2>
 <p>
-Safety is our greatest concern in all places which we operate. With copious amounts of reagents required for our goals we created a web application that manages the material safety data of them in order that they can be stored and accessed on a moments notice. Material safety data sheets are designed to raise awareness of substances and illuminate our members of their heterogeneous natures.
+Safety is our greatest concern. With copious amounts of reagents required for our projects, we created a web application to manage the material safety data sheets of reagents so that that they can be stored and accessed on a moments notice. Material safety data sheets are designed to raise awareness of substances and illuminate our members of their heterogeneous natures.
 </p>
 <p>