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Team:TPHS SanDiego/Project
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Our project focuses on promoter engineering. Our goal is to characterize a set of promoters (of our design) by moving the repressor and/or activator binding sites with respect to the -10 and -35 regions of the promoter. Ideally, we would like to show that by moving an activator binding site it can become a repressor and that by moving a repressor binding site it may become either irrelevant to transcription rate or even boost it. We also want to see if there is a steep decline in repressor/activator function as the binding site move along the promoter or if it is a gradual/linear change. We believe this project could have application to genetic circuits by allowing a single protein to either activate or repress a promoter depending on where the binding sites are placed on the promoter. | Our project focuses on promoter engineering. Our goal is to characterize a set of promoters (of our design) by moving the repressor and/or activator binding sites with respect to the -10 and -35 regions of the promoter. Ideally, we would like to show that by moving an activator binding site it can become a repressor and that by moving a repressor binding site it may become either irrelevant to transcription rate or even boost it. We also want to see if there is a steep decline in repressor/activator function as the binding site move along the promoter or if it is a gradual/linear change. We believe this project could have application to genetic circuits by allowing a single protein to either activate or repress a promoter depending on where the binding sites are placed on the promoter. | ||
<h1>XBa1 Insertion</h1> | <h1>XBa1 Insertion</h1> | ||
| - | + | <h1>LanRFP Insertion by CPEC</h1> | |
| - | + | <h1>Add LanRFP Plasmid into Competent Cells by Tranformation</h1> | |
| - | + | <h1>Add Pcon and LasR by CPEC</h1> | |
| - | + | <h1>Design Promoters with Wobble Extension</h1> | |
| + | <h1>Transform GFP into the RFP Competent Cells</h1> | ||
| + | <h1>Results</h1> | ||
</p> | </p> | ||
Revision as of 22:56, 21 June 2013
Overview
Our project focuses on promoter engineering. Our goal is to characterize a set of promoters (of our design) by moving the repressor and/or activator binding sites with respect to the -10 and -35 regions of the promoter. Ideally, we would like to show that by moving an activator binding site it can become a repressor and that by moving a repressor binding site it may become either irrelevant to transcription rate or even boost it. We also want to see if there is a steep decline in repressor/activator function as the binding site move along the promoter or if it is a gradual/linear change. We believe this project could have application to genetic circuits by allowing a single protein to either activate or repress a promoter depending on where the binding sites are placed on the promoter.XBa1 Insertion
LanRFP Insertion by CPEC
Add LanRFP Plasmid into Competent Cells by Tranformation
Add Pcon and LasR by CPEC
Design Promoters with Wobble Extension
Transform GFP into the RFP Competent Cells
Results
