Overview

Our project focuses on promoter engineering. Our goal is to characterize a set of promoters (of our design) by moving the repressor and/or activator binding sites with respect to the -10 and -35 regions of the promoter. Ideally, we would like to show that by moving an activator binding site it can become a repressor and that by moving a repressor binding site it may become either irrelevant to transcription rate or even boost it. We also want to see if there is a steep decline in repressor/activator function as the binding site move along the promoter or if it is a gradual/linear change. We believe this project could have application to genetic circuits by allowing a single protein to either activate or repress a promoter depending on where the binding sites are placed on the promoter.

XBa1 Insertion

LanRFP Insertion by CPEC

Add LanRFP Plasmid into Competent Cells by Tranformation

Add Pcon and LasR by CPEC

Design Promoters with Wobble Overlap Extension

Transform GFP into the RFP Competent Cells

Results