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Team:The Agency Escondido/project/paper
From 2013hs.igem.org
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Revision as of 17:42, 7 June 2013
Project Description
Our project seeks to create two identical triads of transparent bacteria strains with complementary hydrophobic inducer production to emulate the game of "rock, paper, scissors" (Hydrophobic proteins can move through plasma membranes). Each triad will correspond to a group: group A and group B. Each type of bacteria will produce a specific protein that will act as an inducer to one and only one other bacteria strain(rock strain, paper strain, and scissors strain). Each triad will have different reporter genes designating its champion color. When two bacteria strains are combined in an agar plate, each protein secreted by the victorious strain, when acting as an inducer, changes the color of the cells on the plate identifying the champion.
Contents |
|---|
| Rock |
| Paper |
| Scissors |
Paper
The "paper" bacteria strain will have an GFP (part E0040) and promoter (part I1051). It will contain the Lambda cl repressor gene (part C0051) to induce the "scissors" strain.
Notebook
Grant Hassinger did a run of transforming Cells with I13500 and E0040 but the label wiped off and they became indistinguishable so the work was bleached and disposed of.
May 23
Vansh Singh inoculated eppendorfs with resuspended BBa_I3500 and BBa_R0062.
