Team:Lambert GA/labnotebook

From 2013hs.igem.org

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                 <li><a href="https://2013hs.igem.org/Team:Lambert_GA/activities" accesskey="6" title="">Activities</a></li>               
                 <li><a href="https://2013hs.igem.org/Team:Lambert_GA/activities" accesskey="6" title="">Activities</a></li>               
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<table border="1">
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<tr>
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<th>Date</th>
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<th>Accomplishments</th>
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</tr>
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<tr>
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<td>January 1-15</td>
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<td>Met for the first time during class time as an Instructional Focus.  Discussed project ideas and iGEM in general.  Gave out protocols to study</td>
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</tr>
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<tr>
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<td>January 16-31</td>
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<td>Practiced transformations with p-GLO from Biorad.  Learned about Bacterial Antibiotic resistance using Biorad Antibiotic Resistance Lab. </td>
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</tr>
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<tr>
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<td>February 1-14</td>
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<td>Discussed project ideas.  Planned for visit to Georgia Institute of Technology, Styczynski Lab.  Sold Pretzels for fund raiser.</td>
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<tr>
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<td>February 15-28</td>
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<td>Studied and took test over iGEM protocols and 3A Assembly.  Finalized list of Biobrick parts of interest.  Outlined initial ideas.  Use K410000 to characterize cold shock heat generator.</td>
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</tr>
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<tr>
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<td>March 1-14</td>
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<td>
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<b>Field trip to GA Tech on March 9</b>
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• Rehydrated J23119, J13002, J04450, K410000
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• Miniprepped parts
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• Ran diagnostic gels
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• Sent parts for sequencing
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• Calculated concentration of DNA
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• Met with team members from GATech iGEM.  Discussed the K410000 project. 
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• New idea for project; isolate HybB and make it a Biobrick.  GATech team will send their frozen stocks.
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</td>
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</tr>
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<tr>
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<td>March 15-28</td>
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<td>Worked on research for the new parts.  Wrote Project proposal.</td>
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</tr>
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<td>April 1-24</td>
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<td>
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<b>April 1st</b>
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• Transformed again
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• Made Amp plates
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• Cataloged Gaucher 2010 box
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• Plated the HypB with RFP on Amp plates
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<b>April 4th</b>
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• Liquid culture results were analyzed
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• Took the HypB samples and mixed with Glycerol (Glyceroled Bio Bricks) and placed them in 80oC freezer
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• Streaked LB plates with HypB samples, promoter, and K410000 (bio brick)
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• Made more LB plates
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• Miniprepped hypB parts and K410 from Gaucher box
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• Calculated the DNA concentration
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• Calculated concentrations for sequencing
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• Wrote instructions for Cold Shock
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<b>April 24th</b>
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• Nanodropped with DNA concentrations
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• Ligation ( Protocol)
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• Digest of RFP, hypB, psBIC3, 410
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</td>
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</tr>
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<tr>
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<td>May 1- May 14</td>
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<td>
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• Transformation
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• RFP  control and 410 RFP grew but only RFP has an expression
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• Ran transformation efficiency test with Protocol
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• Prep for experiment- plated 10 plates and inoculated 16 tubes
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• Experiment for temperature variation was successful but did not prove hypothesis</td>
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</tr>
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<tr>
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<td>May 30th</td>
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<td>Several of our members attended a safety training meeting, and are now certified in lab safety courses.</td>
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</table>

Revision as of 13:39, 11 June 2013