Team:Lambert GA/labnotebook

From 2013hs.igem.org

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Revision as of 13:40, 11 June 2013

Lab Notebook

Date	Accomplishments
January 1-15	Met for the first time during class time as an Instructional Focus. Discussed project ideas and iGEM in general. Gave out protocols to study
January 16-31	Practiced transformations with p-GLO from Biorad. Learned about Bacterial Antibiotic resistance using Biorad Antibiotic Resistance Lab.
February 1-14	Discussed project ideas. Planned for visit to Georgia Institute of Technology, Styczynski Lab. Sold Pretzels for fund raiser.
February 15-28	Studied and took test over iGEM protocols and 3A Assembly. Finalized list of Biobrick parts of interest. Outlined initial ideas. Use K410000 to characterize cold shock heat generator.
March 1-14	Field trip to GA Tech on March 9 • Rehydrated J23119, J13002, J04450, K410000 • Miniprepped parts • Ran diagnostic gels • Sent parts for sequencing • Calculated concentration of DNA • Met with team members from GATech iGEM. Discussed the K410000 project. • New idea for project; isolate HybB and make it a Biobrick. GATech team will send their frozen stocks.
March 15-28	Worked on research for the new parts. Wrote Project proposal.
April 1-24	April 1st • Transformed again • Made Amp plates • Cataloged Gaucher 2010 box • Plated the HypB with RFP on Amp plates April 4th • Liquid culture results were analyzed • Took the HypB samples and mixed with Glycerol (Glyceroled Bio Bricks) and placed them in 80oC freezer • Streaked LB plates with HypB samples, promoter, and K410000 (bio brick) • Made more LB plates • Miniprepped hypB parts and K410 from Gaucher box • Calculated the DNA concentration • Calculated concentrations for sequencing • Wrote instructions for Cold Shock April 24th • Nanodropped with DNA concentrations • Ligation ( Protocol) • Digest of RFP, hypB, psBIC3, 410
May 1- May 14	• Transformation • RFP control and 410 RFP grew but only RFP has an expression • Ran transformation efficiency test with Protocol • Prep for experiment- plated 10 plates and inoculated 16 tubes • Experiment for temperature variation was successful but did not prove hypothesis
May 30th	Several of our members attended a safety training meeting, and are now certified in lab safety courses.

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