Team:St Pauls London/Safety

From 2013hs.igem.org

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|BBa_C0072
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|Lacl gene
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|P22 bacteriophage
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|E
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|BBa_E1010
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|RFP gene
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|Discosoma striata
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|F
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|BBa_B0015
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|Terminator
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|E. coli strain NEB 10-beta
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|J
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|BBa_K091104
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|Promoter
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|E. coli strain NEB 10-beta
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|K
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|BBa_B0034
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|RBS
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|E. coli strain NEB 10-beta
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|L
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|BBa_E0030
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|YFP gene
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|Aequorea victoria
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Revision as of 21:10, 16 June 2013

Safety

1. All procedures were carried out in a clean area. This area was set up as detailed below, and ensured that no external microorganisms contaminated plates or tubes.
  • Bunsen burners were on at all times, such that a updraft was always present.
  • Prior to any procedures taking place, the entire area which was to be used was scrubbed down with antiseptic.
2. All waste was disposed of in separate, marked bins. The contents of these were then incinerated.
3. Antimicrobial hand wash was systematically used upon entering and leaving the lab to ensure no genetically modified microorganisms could be released outside of the one projects labs in which procedures were carried out.
4. Sterile gloves were worn at all times.
5. Safety glasses were worn at all times.
Letter Code for Part BioBrick Reference Function Organism Derived From Risk Group
A BBa_K418003 Promoter E. coli strain NEB 10-beta 1
B BBa_B0033 RBS E. coli strain NEB 10-beta 1
C BBa_C0040 TetR gene E. coli strain NEB 10-beta 1
D BBa_C0072 Lacl gene P22 bacteriophage 1
E BBa_E1010 RFP gene Discosoma striata 1
F BBa_B0015 Terminator E. coli strain NEB 10-beta 1
J BBa_K091104 Promoter E. coli strain NEB 10-beta 1
K BBa_B0034 RBS E. coli strain NEB 10-beta 1
L BBa_E0030 YFP gene Aequorea victoria 1
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