<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;HybB was synthesized for the first time by the 2005 UCSF iGEM team. The 2006 MIT iGEM team standardized HybB and submitted it to the parts registry. HybB has the part number J45503 and is not available from the Parts Registry. Georgia Tech’s 2010 iGEM team engineered a chassis to include HybB, OmpA, and AOX.  The 2011 Leuven iGEM team reported, “We see that promoter activity is induced when cells are transferred to 25°C and even when they are put in an ice bath (4°C). Unfortunately, however, cells that are kept at 37°C also display an increase in promoter activity, indicating leakiness in the system.” The 2011 Groningen team reported that the HybB Promoter did not work. Through sequencing, they proved that HybB was found in the cells, and they ran the reactions at the same temperatures.However, they could not get the same results as the Leuven team. Due to these more recent findings, we are not sure whether HybB works or not.

<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;HybB was synthesized for the first time by the 2005 UCSF iGEM team. The 2006 MIT iGEM team standardized HybB and submitted it to the parts registry. HybB has the part number J45503 and is not available from the Parts Registry. Georgia Tech’s 2010 iGEM team engineered a chassis to include HybB, OmpA, and AOX.  The 2011 Leuven iGEM team reported, “We see that promoter activity is induced when cells are transferred to 25°C and even when they are put in an ice bath (4°C). Unfortunately, however, cells that are kept at 37°C also display an increase in promoter activity, indicating leakiness in the system.” The 2011 Groningen team reported that the HybB Promoter did not work. Through sequencing, they proved that HybB was found in the cells, and they ran the reactions at the same temperatures.However, they could not get the same results as the Leuven team. Due to these more recent findings, we are not sure whether HybB works or not.

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" 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" 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