Team:TPHS SanDiego/Project

From 2013hs.igem.org

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* jQuery Nivo Slider v2.6
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* http://nivo.dev7studios.com
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*
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* Copyright 2011, Gilbert Pellegrom
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* Free to use and abuse under the MIT license.
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* http://www.opensource.org/licenses/mit-license.php
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*
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* March 2010
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*/
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<style type="text/css">
<style type="text/css">
body {background: #000000}
body {background: #000000}

Revision as of 17:54, 18 June 2013

<style type="text/css"> body {background: #000000} </style>

Our project focuses on promoter engineering. Our goal is to characterize a set of promoters (of our design) by moving the repressor and/or activator binding sites with respect to the -10 and -35 regions of the promoter. Ideally, we would like to show that by moving an activator binding site it can become a repressor and that by moving a repressor binding site it may become either irrelevant to transcription rate or even boost it. We also want to see if there is a steep decline in repressor/activator function as the binding site move along the promoter or if it is a gradual/linear change. We believe this project could have application to genetic circuits by allowing a single protein to either activate or repress a promoter depending on where the binding sites are placed on the promoter.

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