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Team:The Agency Escondido/notebook
From 2013hs.igem.org
- Notebook
- Safety
April 9
Andrew Buss prepared 120 mL of LB broth
April 10
Andrew Buss prepared two LB plates without antibiotics, using our LB plate protocol, rev 72.
Andrew Segina inoculated two ampicillin/kanamycin LB plates with E. coli containing Parts A and B from the 3A assembly kit
Andrew Buss inoculated an SOB plate with NEB10 competent E. coli
April 11
Andrew Segina inoculated an SOB plate with NEB10 competent E. coli
The plates previously inoculated with E. coli with parts A and B do not show visible growth
Andrew Segina prepared 1 L of YPD broth without dextrose
April 12
Andrew Buss inspected plates at 8:15 AM. Both NEB10 plates showed visible growth. Neither plate with Part A or B displayed growth.
Andrew Buss prepared four YPD plates from the 1L stock and mixed 4 grams of table sugar before pouring
Andrew Buss inoculated two LB tubes each with E. coli with parts A and B directly from the agar stabs included in the 3A assembly kit. The intention was to diagnose the lack of growth on the A and B plates over two days.
Andrew Buss inspected plates again around 1:00 PM. NEB10 plates showed additional growth, and the plate with Part A contained visible colonies. The plate with Part B did not show growth, however.
Grant Hassinger mixed 100 mL of LB broth and began an autoclave cycle with the media
Vansh Singh inoculated a tube of ampicillin/kanamycin LB broth from the 3A assembly kit with a colony from the Part A plate
Vansh Singh prepared a tube of ampicillin/kanamycin LB broth from the 3A assembly kit without inoculation. The tube was placed into the refrigerator
Andrew Buss inoculated a tube of LB broth with a colony from the NEB10 plate from April 10
April 13 labeled media
| Andrew Segina | LB Plate | Part A from kit | Ampicillin, kanamycin | |
| Andrew Segina | LB Plate | Part B from kit | Ampicillin, kanamycin | |
| Andrew Buss | SOB Plate | NEB 10 from kit | ||
| Andrew Segina | SOB Plate | NEB 10 from kit | ||
| Andrew Buss | LB Tube | Part A from kit | Incubating in dry bath | |
| Andrew Buss | LB Tube x2 | Part A from kit | Incubating in dry bath | |
| Andrew Buss | LB Tube x2 | Part B from kit | Incubating in dry bath | |
| Vansh Singh | LB Tube | Part A from plate | Incubating in dry bath | |
| Andrew Buss | LB Tube | NEB 10 from 4/10 SOB plate | Incubating in dry bath |
April 13 available media
| Andrew Segina | 800 mL YPD | Lacks dextrose |
| Andrew Buss | 4 YPD plates | |
| Andrew Segina | 8 LB plates | |
| Andrew Buss | ~40mL LB broth | |
| Vansh Singh | ~5mL LB broth | Ampicillin, kanamycin |
| Grant Hassinger | 100mL LB broth | |
| Grant Hassinger | 100mL LB broth |
