Team:Deerfield MA
From 2013hs.igem.org
- a team description
- project description
- safety information (did your team take a safety training course? were you supervised in the lab?)
- team attribution (who did what part of your project?)
- lab notebook
- sponsor information
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Team Deerfield_MA |
Official Team Profile |
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Contents |
Team
Tell us about your team, your school!
Mercury poisoning is a serious problem, which can devastate fish populations and ecosystems. Humans who consume contaminated fish may experience negative affects as a result. The Deerfield, MA iGEM Team has proposed a remedy to this issue with the aid of a biosensor. Our goal is to manipulate the system found in part K346001 that uses the merR gene to detect mercury, and then either promote or suppress a reporter gene. Previously GFP has been used as the reporter, but our plan is to test a few different reporters including Lumazine (K216007) and LacZ (I0500). We will test for transform efficiency, reporter strength, and objective overall succes to find which reporter is most effective in this system. Once these transformed cells are produced, someone (i.e. a fisherman) would be able to take a water sample in the area he or she plans to fish, allow the cells to inoculate overnight in the water sample, and then discover whether mercury is present in the water source. Depending on the output, the cells may be able to detect the severity of the contamination.
Notebook
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Results/Conclusions
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Safety
What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
Attributions
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Human Practices
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Fun!
What was your favorite team snack?? Have a picture of your team mascot?
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