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Team:Shenzhen SFLS/Project
From 2013hs.igem.org
- a team description
- project description
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- team attribution (who did what part of your project?)
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| Official Team Profile |
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Contents |
Team
Peilin Li: the instructor of SFLS students.
Kang Kang: the advisor of SFLS students.
Hekang Jia: the captain of SFLS students.
Biwei Zheng: the vice captain of SFLS students.
Project
Student at SFLS want to use an enzyme known as “polyphosphate kinase”（PPK）to governance the eutrophication of water bodies. We know that Inorganic phosphate (Pi) is recognized as one of the major nutrients contributing to the outbreak of red tide.We have found that PPK is known to digest Inorganic phosphate (Pi). There is a small amount of it in e. coli and we want to copy the gene of PPK in the e.coli to make it more efficient,so the role of PPK will be more obvious Then we will use the GFP to express the result of the digest of PPK.We want to make two device in a gene circut to deal with this problem.
Device 1: We use a limitable promoter which would be limited by high concentrations of Pi, then the GFP in this device won't light and a protein we add in the device won't breed .
Device 2: If the protein didn't breed, the promoter in Device 2 will be induced and the PPK gene will work, it will digest Pi. When the concentration of phosphate become low, devive 2 will stop to digest Pi and the promoter in device 1 will be induced, then the GFP in device 1 will light. That means the work has finished.
( We will put on our picture of gene circuts in few days)
Notebook
Show us how you spent your days.
Results/Conclusions
What did you achieve over the course of your semester?
Safety
What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
Attributions
Who worked on what?
Human Practices
What impact does/will your project have on the public?
Fun!
What was your favorite team snack?? Have a picture of your team mascot?
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