| We are the Lambert High School iGEM team from Forsyth County, Georgia. We are honored to be the first high school iGEM team from Georgia, and this is our first year in the competition.
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Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs) | |
| Team Lambert_GA |
| Official Team Profile |
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Contents |
We are Georgia's first high school iGEM team. We are from Lamber High School in Forsyth County, Georgia, just outside Atlanta. This is the first year that our school has had an iGEM program, and we are very excited to be part of this group. Our instructor is Mrs. Janet Standeven, who teaches biology and environmental science at Lambert. We are also being sponsored by Dr. Mark Styczynski from Georgia Tech's Chemical Engineering department.
The members of our iGEM team are very enthusiastic about biotechnology and molecular engineering and are glad to be presented with the opportunity to gain valuable experience in this ever-expanding bio technical field and to have been able to work in doctorate labs at the prestigious Georgia Institute of Technology being just high school students.
In 2010, Georgia Institute of Technology’s iGem team submitted the part K410000, a periplasmic heat generator made with HybB and OmpA and AOX. The following year several teams used HybB in their projects with mixed results. Our project is the continuation these projects and the characterization of HybB. To do this we will put RFP under HybB promotion. In addition to we will put OmpA AOX under three constituitive promoters. The purpose is to determine whether the strength of constituitive promoters will change the amount of heat released by generator OmpA AOX. With HybB we are hoping to get reliable results from cold shock treatment.
By engineering a reliable coldshock promoter, we can provide a switch to turn on gene expression in order to control cell protein expression using temperature.
January 1-15---Met for the first time during class time as an Instructional Focus. Discussed project ideas and iGEM in general. Gave out protocols to study
• Rehydrated J23119, J13002, J04450, K410000
• Miniprepped parts
• Ran diagnostic gels
• Sent parts for sequencing
• Calculated concentration of DNA
• Met with team members from GATech iGEM. Discussed the K410000 project.
• New idea for project; isolate HybB and make it a Biobrick. GATech team will send their frozen stocks.
April 1st:
• Transformed again
• Made Amp plates
• Cataloged Gaucher 2010 box
• Plated the HypB with RFP on Amp plates
April 4th:
• Liquid culture results were analyzed
• Took the HypB samples and mixed with Glycerol (Glyceroled Bio Bricks) and placed them in 80oC freezer
• Streaked LB plates with HypB samples, promoter, and K410000 (bio brick)
• Made more LB plates
• Miniprepped hypB parts and K410 from Gaucher box
• Calculated the DNA concentration
• Calculated concentrations for sequencing
• Wrote instructions for Cold Shock
April 24th:
• Nanodropped with DNA concentrations
• Ligation ( Protocol)
• Digest of RFP, hypB, psBIC3, 410
• Transformation
• RFP control and 410 RFP grew but only RFP has an expression
• Ran transformation efficiency test with Protocol
• Prep for experiment- plated 10 plates and inoculated 16 tubes
• Experiment for temperature variation was successful but did not prove hypothesis
What did you achieve over the course of your semester?
All members of the team participated in Lab safety training at our High School. In addition 5 members of the team completed Right to Know, General Lab Safety, Biohazard training and Recombinant DNA safety training offered by Georgia Institute of Technology.
Whenever the team was performing procedures, everybody wore safety goggles and gloves. We followed procedures very carefully, and we were circumspect with all procedures and cleanup. We took a safety quiz at the beginning of the team formation. We were supervised by Mrs. Standeven, Dr. Styczynski, and Mrs. Cochran the entire time we were in the Georgia Tech lab.
Who worked on what?
Everyone in the Lambert iGem team is motivated and dedicated to enhance the team and our projects. Mrs.Standeven, has dedicated many hours of her day to help prepare for upcoming labs.
In our inaugural year we focused on educating our teachers, fellow classmates and administration about the purpose of iGEM. We recruited members from the 9-12 grade which gave a large audience with whom to spread the information. Synthetic Biology as a topic was a new idea even to some of our teachers. Several members of our team volunteer at both Science Olympiad camps and STEM camps. They brought activities we had performed as team to the younger audiences in order to familiarize them with DNA.
Our favorite team snack is Pretzels. King of Pops was a special treat in Atlanta on a work day.
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