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From 2013hs.igem.org

Below are some resources that may come in handy as you're working on your projects for iGEM 2013 HS!

Wiki editing

• MediaWiki has detailed instructions for wiki editing, including the following:
• General Formatting
• Image Editing

Purchasing laboratory reagents and materials

• Fisher Scientific offers a wide array of common laboratory materials and reagents
• Sigma Aldrich offers many chemical compounds, including antibiotics

Databases and websites to explain concepts:

• Parts Registry Help page is where you can find information about BioBrick Parts and how to use them
• OpenWetWare is a great source for protocols and other lab-related information
• CompuBioTicDB is a database of biological components for synthetic biology

Tutorials:

• Polymerase chain reaction (PCR) (University of Utah)
• Primer design and ordering (OpenWetWare)
• Ligation and plasmid construction (BioBuilder)

Software tools:

• FAQ section on iGEM.org answers questions about wiki-editing
• TinkerCell is a free "Computer-Aided Design software tool for Synthetic Biology"
• GENtle is free software that allows users to edit DNA sequences, generate plasmid maps, and more
• SecondLife notes for using this program during the team leader meetings and Jamboree!

Protocols:

• Making competent cells (Registry)
• Competent cell transformation (Registry)
• Ligation (Registry)
• Restriction digest (Registry)
• Transformation lab for students
• Ligation and Transformation module (Bio-Rad)
• Yeast transformation (University of Manitoba)