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Team:TPHS SanDiego
From 2013hs.igem.org
In an effort to expand the toolkit available to synthetic biologists, we've taken a system natively responsible for transcriptional activation and modified it to control transcriptional repression. The LasR system from Pseudomonas Aeruginosa requires the presence of a small molecule, C12-3-oxo-AHL, to induce activation of the Plas promoter. By modifying the -10 and -35 sites of the promoter, as well as shifting the location of the LasR binding sites, the new Plas promoter (Plas*) was changed from an inducible to a repressible promoter. Through adding this second functionality, the Plas* promoter could be used in conjunction with a wildtype Plas promoter to control two separate genes whose expression levels are always out of sync. Furthermore, if the bacteria are transfected with a plasmid encoding LasI, the bacteria will be able to turn off gene expression at a critical population density, instead of only being able to turn on gene expression.
Proteins discovered in Jellyfish that have the ability to emit fluorescence.
A clever way to normalize protein expression across cell populations.
A quorum sensing system from Pseudomonas aeruginosa.
Re-engineering the Plas promoter's response to LasR.
The Torrey Pines High School iGEM team would like to thank New England Biolabs, the UCSD Biodynamics Labratory, and Mr. Brinn Belyea.
